Determination of Leaf Volatile Components from Five Phoebe Species

doi:10.16420/j.issn.0513-353x.2020-0987

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1552-1564.doi: 10.16420/j.issn.0513-353x.2020-0987

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Determination of Leaf Volatile Components from Five Phoebe Species

CHU Sasa¹, FAN Xiangzhen², QIU Zhaokai¹, YANG Qi¹, ZHANG Yuting¹, WANG Yang¹, XU Xiaoshan¹, TONG Zaikang¹, ZHANG Junhong¹^,^*()

¹Zhejiang A & F University,State Key Laboratory of Subtropical Silviculture,Hangzhou 311300,China
²Forestry Working Station of Hushan in Suichang County,Lishui,Zhejiang 323308,China

Received:2021-04-19 Revised:2021-06-21 Online:2021-08-25 Published:2021-09-06
Contact: ZHANG Junhong E-mail:zhangjunhong@zafu.edu.cn

Abstract

Abstract:

The Phoebe species are valuable ornamental trees,with abundant volatile compounds in the leaves. In order to determine the dynamic changes of volatile compounds in the leaves of five Phoebe species,the headspace-solid phase microextraction（SPME）,separated and analyzed by gas chromatography-mass spectrometry（GC-MS）. A total of 80 volatile compounds were identified from five Phoebe species,and 52,55,41,56,and 46 compounds were detected from P. chekiangensis,P. bournei, P. sheareri,P. zhennan f. oblong,and P. zhennan f. elliptical,respectively. A total of 70 and 64 volatile compounds were identified from four directions of P. chekiangensis leaves in summer and autumn,respectively. In summer,the maximum variety of compounds was detected in western leaves,while in the autumn the maximum variety was detected in northern leaves. Further,OPLS-DA analysis showed that the volatile compounds were differed between northern and southern leaves,while the western and eastern leaves shared similar compounds. Moreover,we identified six major components,including α-copaene,and α-humulene significantly diverged in leaves grown under four temperatures. In particular,the compound contents under 45 ℃ significantly differed from the other three temperatures. Additionally,the volatile components showed diurnal variation in P. chekiangensis leaves. Eight compounds including α-copaene,β-cadinene and γ-muurolene peaked at 12：00 or 14：00,while ten compounds such as β-caryophyllene,(+)-germacrene D and β-elemene had the lowest content at 12：00 or 14：00. These results provided theoretical guidance on developing and utilizing essential oils from the leaves of P. chekiangensis and other Phoebe species,and the forest rehabilitation at the Phoebe principal stand.

Key words: Phoebe, Phoebe chekiangensis, volatile components, HS-SPME, GC-MS

CLC Number:

CHU Sasa, FAN Xiangzhen, QIU Zhaokai, YANG Qi, ZHANG Yuting, WANG Yang, XU Xiaoshan, TONG Zaikang, ZHANG Junhong. Determination of Leaf Volatile Components from Five Phoebe Species[J]. Acta Horticulturae Sinica, 2021, 48(8): 1552-1564.

Figures/Tables 7

References 31

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类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	相对含量/% Relative content
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	浙江楠 P. chekian- gensis	小叶桢楠 P. zhennan f. oblong	大叶桢楠 P. zhennan f. elliptical	紫楠 P. sheareri	闽楠 P. bour- nei
萜烯类（单萜）	A1	933	蒎烯Pinene	3.37	1.44	0.88	8.25	0.44
Terpenes	A2	943	α-蒎烯α-Pinene	0.32	0.21	0.43	0.82	5.09
（monoterpene）	A3	981	β-蒎烯β-Pinene	–	–	–	–	3.57
	A4	949	莰烯Camphene	0.71	0.63	0.27	0.97	1.16
	A5	977	(-)-β-蒎烯 (-)-β-Pinene	1.48	0.62	0.45	5.82	0.27
	A6	997	月桂烯 Myrcene	0.27	0.27	–	0.57	0.54
	A7	1 007	水芹烯 Phellandrene	0.18	0.36	–	–	0.16
	A8	1 024	S-(-)-柠檬烯 S-(-)-Limonene	–	–	–	–	0.40
	A9	1 057	(+)-柠檬烯 (+)-Limonene	1.11	0.43	0.13	1.85	–
	A10	1 044	(E)-β-罗勒烯 (E)-β-Ocimene	0.18	1.66	–	–	1.14
	A11	1 054	(Z)-13,7-dimethyl-3,6-octatriene	0.20	1.59	0.37	0.45	1.46
	A12	1 093	萜品油烯 Terpinolene	–	0.11	–	0.12	–
	A13	1 134	别罗勒烯(E,Z)-2,6- Dimethylocta-2,4,6-triene	0.19	1.54	0.35	0.81	0.61
萜烯类（倍半萜） Terpenes （sesquiterpene）	A14	1 331	1-ethenyl-1-methyl-4-propan-2-ylidene-2-prop-1-en-2-ylcyclohexane	5.60	6.16	6.50	0.47	6.74
	A15	1 344	(-)-α-荜澄茄油烯 (-)-α-Cubebene	1.54	5.60	7.00	0.61	2.55
	A16	1 360	(+)-长叶环烯 (+)-Longicyclene	–	–	–	–	0.38
	A17	1 363	(+)-环苜蓿烯 (+)-Cyclosativene	–	0.41	0.60	–	–
	A18	1 370	1,2,4a,5,6,8a-hexahydro-1-isopropyl-4,7-dimethylnaphthalene	–	–	–	–	0.15
	A19	1 376	α-古巴烯 α-Copaene	7.35	19.05	22.94	2.48	12.66
	A20	1 384	β-波旁烯 β-Bourbonene	1.40	2.11	3.32	0.41	2.48
	A21	1 390	β-荜澄茄油烯 β-Cubebene	3.34	8.29	3.85	8.80	5.13
	A22	1 393	β-榄香烯β-Elemene	6.84	2.79	2.10	6.40	4.02
	A23	1 407	2,6-二甲基-6-（4-甲基- 3-戊烯基） 2,6-Dimethyl-6-(4-methyl-3-pentenyl)bicyclo[3.1.1]hept-2-ene	0.65	0.52	0.24	0.29	–
	A24	1 411	(-)-α-古芸烯 (-)-α-Gurjunene	0.35	1.64	1.09	0.64	1.31
	A25	1 418	（1S,5S）-4,6-二甲基-6- （4-甲基戊-3-烯基）双环 [3.1.1]庚-3-烯 (1S,5S)-4,6-Dimethyl-6-(4-methylpent-3-enyl)bicyclo[3.1.1]hept-3-ene	0.65	0.29	0.35	3.28	–
	A26	1 422	β-石竹烯β-Caryophyllene	8.01	3.33	5.58	10.42	5.70
	A27	1 448	马兜铃烯Aristolene	2.14	1.47	2.84	0.17	2.14
	A28	1 454	γ-广藿香烯γ-Patchoulene	1.68	1.06	2.32	–	1.58
	A29	1 459	α-葎草烯α-Humulene	1.87	2.91	2.48	24.41	3.90
	A30	1 463	β-倍半水芹烯 β-Sesquiphellandrene	0.79	0.11	–	–	–
	A31	1 466	香树烯(-)-Alloaromadendrene	0.57	1.62	2.06	–	1.02
	A32	1 475	α-Himachalene	–	–	–	–	0.53
	A33	1 494	(+)-β-瑟林烯 (+)-β-Selinene	3.76	2.67	0.54	1.87	3.01
	A34	1 489	右旋大根香叶烯 (+)- Germacrene D	6.50	3.70	9.41	2.29	6.13
	A35	1 498	愈创木烯Guaiacene	3.09	1.58	0.14	0.70	1.19
	A36	1 413	(+)-γ-古芸烯 (+)-γ-Gurjunene	–	–	–	–	3.71
	A37	1 428	(-)-β-花柏烯 (-)-β-Chamigrene	–	–	–	–	2.50
	A38	1 435	γ-榄香烯γ-Elemene	–	–	–	–	0.67
	A39	1 505	1-ethenyl-1-methyl-2-(1-methylethenyl)-4-(1-methylethylidene)-cyclohexane	5.09	7.03	6.42	0.42	6.43
	A40	1 510	(+)-香橙烯 (+)-Aromadendrene	0.66	0.67	1.00	0.13	–
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	相对含量/% Relative content
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	浙江楠 P. chekian- gensis	小叶桢楠 P. zhennan f. oblong	大叶桢楠 P. zhennan f. elliptical	紫楠 P. sheareri	闽楠 P. bour- nei
	A41	1 490	-Azulene,1,2,3,4,5,6,7,8-octahydro-1,4-dimethyl-7-(1-methylethylidene)-, (1S,4S)-	–	0.13	–	0.70	–
	A42	1 523	(+)-γ-荜澄茄烯 (+)-γ-Cadinene	–	–	–	–	0.53
	A43	1 533	δ-荜澄茄烯 δ-Cadinene	6.01	1.87	3.57	1.12	1.81
	A44	1 609	α-广藿香烯 α-Patchoulene	0.13	0.41	0.11	0.25	0.42
	A45	1 621	环氧化蛇麻烯Ⅱ Humulene1,2-epoxide	0.18	0.27	0.14	0.55	–
醛类 Aldehydes	B1	1 645	2-甲基-4-戊醛 2-Methylpent-4-enal	1.49	0.69	0.25	0.10	0.51
	B2	1 124	壬醛 Nonanal	0.13	–	–	–	0.12
	B3	1 210	癸醛 Decanal	0.17	0.35	0.27	–	–
	B4	1 305	10-十一烯醛10-Undecenal	–	–	–	–	0.13
	B5	1 310	十一醛 Undecanal	–	0.33	–	–	0.13
	B6	1 412	十二醛 Lauryl aldehyde	0.16	1.02	0.13	–	–
	B7	1 520	十四烷醛 Tetradecanal	–	–	–	0.17	–
醇类 Alcohols	C1	2 510	反式-3-己烯-1-醇 Trans-3-Hexen-1-ol	2.08	1.57	1.02	1.51	1.50
	C2	2 738	3-甲基-4-戊醇 3-Methylpent-4-en-1-ol	0.22	0.43	–	0.28	–
	C3	1 409	环十二醇Cyclododecanol	–	–	–	–	0.39
	C4	2 714	叶醇cis-3-Hexen-1-ol	0.17	–	0.36	–	–
萜烯醇类	D1	1 035	桉叶油醇 Cineole	0.10	–	–	3.97	–
Terpene alcohols	D2	1 108	芳樟醇 Linalool	0.43	–	–	–	–
	D3	1 094	橙花醇 Nerol	–	–	–	–	0.14
	D4	1 295	紫苏醇 Perillyl alcohol	–	–	–	–	0.13
	D5	1 540	α-檀香醇 α-Santalol	–	0.13	0.11	–	–
	D6	1 526	α-人参烯 α-Panasinsanene	0.11	–	–	–	–
	D7	1 584	4(15),5,10(14)-大根香叶三烯-1-醇 4(15),5,10(14)-Germacratrien-1-ol	–	0.14	0.13	–	–
	D8	1 590	桉油烯醇 Spathulenol	–	0.68	0.22	–	0.47
	D9	1 633	Β-桉叶醇 β-Eudesmol	0.16	0.10	–	–	–
	D10	1 650	1aS,4aS,7R,7aS,7bS)-1,1,7- Trimethyl-4-methylidene-1a,2,3, 4a,5,6,7a,7b-octahydrocyclopropa [h]azulen-7-ol	–	0.11	–	–	–
酯类 Esters	E1	1 027	(E)-3-己烯-1-醇乙酸酯 Trans-3-hexenyl acetate	0.26	0.21	0.54	0.49	3.57
	E2	1 028	乙酸叶醇酯 cis-3-Hexenyl acetate	–	0.10	–	–	–
萜烯酯类	F1	1 265	乙酸芳樟酯 Linalyl acetate	0.23	0.41	0.17	–	0.91
Terpene esters	F2	1 299	乙酸龙脑酯 Bornyl acetate	3.70	0.98	0.62	5.10	–
	F3	1 292	左旋乙酸冰片酯 L-Bornyl acetate	–	–	–	–	2.24
萘类 Naphthalenes	G1	1 517	(1aR)-1aβ,2,3,3a,4,5,6,7bβ- Octahydro-1,1,3aβ,7-tetramethyl- 1H-cyclopropa[a]naphthalene	1.85	0.89	1.59	0.35	–
	G2	1 542	1,2,3,4,4a,7-Hexahydro-1,6- dimethyl-4-(1-methylethyl)- naphthalene	0.35	0.30	0.29	–	0.20
	G3	1 548	1,2,4a,5,6,8a-Hexahydro-4,7- dimethyl-1-(1-methylethyl) napthtalene	0.15	0.14	0.24	0.11	0.41
	G4	1 578	(8aα)-Decahydro-1α-isopropyl- 3aα-methyl-7-methylene-4α,8α- epoxyazulene	–	0.19	0.10	–	0.42
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	相对含量/% Relative content
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	浙江楠 P. chekian- gensis	小叶桢楠 P. zhennan f. oblong	大叶桢楠 P. zhennan f. elliptical	紫楠 P. sheareri	闽楠 P. bour- nei
烷烃类 Alkanes	H1	1 303	正十三烷Tridecane	–	–	–	0.10	0.13
	H2	1 308	环己烷Cyclohexane	–	–	–	–	0.67
	H3	1 556	(E)-2-(6,10-Timethylundeca-1,5,9- trien-2-yl)oxirane	0.11	–	–	–	–
有机酸 Organic acid	I1	1 283	(1R,4S)-1,7,7-Trimethylbicyclo [2.2.1]heptan-2-yl acetate	–	–	–	–	0.33
氧化物 Oxide	J1	1 595	石竹素Caryophyllene oxide	0.49	0.34	0.22	0.21	0.36

类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	相对含量/% Relative content
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	浙江楠 P. chekian- gensis	小叶桢楠 P. zhennan f. oblong	大叶桢楠 P. zhennan f. elliptical	紫楠 P. sheareri	闽楠 P. bour- nei
萜烯类（单萜）	A1	933	蒎烯Pinene	3.37	1.44	0.88	8.25	0.44
Terpenes	A2	943	α-蒎烯α-Pinene	0.32	0.21	0.43	0.82	5.09
（monoterpene）	A3	981	β-蒎烯β-Pinene	–	–	–	–	3.57
	A4	949	莰烯Camphene	0.71	0.63	0.27	0.97	1.16
	A5	977	(-)-β-蒎烯 (-)-β-Pinene	1.48	0.62	0.45	5.82	0.27
	A6	997	月桂烯 Myrcene	0.27	0.27	–	0.57	0.54
	A7	1 007	水芹烯 Phellandrene	0.18	0.36	–	–	0.16
	A8	1 024	S-(-)-柠檬烯 S-(-)-Limonene	–	–	–	–	0.40
	A9	1 057	(+)-柠檬烯 (+)-Limonene	1.11	0.43	0.13	1.85	–
	A10	1 044	(E)-β-罗勒烯 (E)-β-Ocimene	0.18	1.66	–	–	1.14
	A11	1 054	(Z)-13,7-dimethyl-3,6-octatriene	0.20	1.59	0.37	0.45	1.46
	A12	1 093	萜品油烯 Terpinolene	–	0.11	–	0.12	–
	A13	1 134	别罗勒烯(E,Z)-2,6- Dimethylocta-2,4,6-triene	0.19	1.54	0.35	0.81	0.61
萜烯类（倍半萜） Terpenes （sesquiterpene）	A14	1 331	1-ethenyl-1-methyl-4-propan-2-ylidene-2-prop-1-en-2-ylcyclohexane	5.60	6.16	6.50	0.47	6.74
	A15	1 344	(-)-α-荜澄茄油烯 (-)-α-Cubebene	1.54	5.60	7.00	0.61	2.55
	A16	1 360	(+)-长叶环烯 (+)-Longicyclene	–	–	–	–	0.38
	A17	1 363	(+)-环苜蓿烯 (+)-Cyclosativene	–	0.41	0.60	–	–
	A18	1 370	1,2,4a,5,6,8a-hexahydro-1-isopropyl-4,7-dimethylnaphthalene	–	–	–	–	0.15
	A19	1 376	α-古巴烯 α-Copaene	7.35	19.05	22.94	2.48	12.66
	A20	1 384	β-波旁烯 β-Bourbonene	1.40	2.11	3.32	0.41	2.48
	A21	1 390	β-荜澄茄油烯 β-Cubebene	3.34	8.29	3.85	8.80	5.13
	A22	1 393	β-榄香烯β-Elemene	6.84	2.79	2.10	6.40	4.02
	A23	1 407	2,6-二甲基-6-（4-甲基- 3-戊烯基） 2,6-Dimethyl-6-(4-methyl-3-pentenyl)bicyclo[3.1.1]hept-2-ene	0.65	0.52	0.24	0.29	–
	A24	1 411	(-)-α-古芸烯 (-)-α-Gurjunene	0.35	1.64	1.09	0.64	1.31
	A25	1 418	（1S,5S）-4,6-二甲基-6- （4-甲基戊-3-烯基）双环 [3.1.1]庚-3-烯 (1S,5S)-4,6-Dimethyl-6-(4-methylpent-3-enyl)bicyclo[3.1.1]hept-3-ene	0.65	0.29	0.35	3.28	–
	A26	1 422	β-石竹烯β-Caryophyllene	8.01	3.33	5.58	10.42	5.70
	A27	1 448	马兜铃烯Aristolene	2.14	1.47	2.84	0.17	2.14
	A28	1 454	γ-广藿香烯γ-Patchoulene	1.68	1.06	2.32	–	1.58
	A29	1 459	α-葎草烯α-Humulene	1.87	2.91	2.48	24.41	3.90
	A30	1 463	β-倍半水芹烯 β-Sesquiphellandrene	0.79	0.11	–	–	–
	A31	1 466	香树烯(-)-Alloaromadendrene	0.57	1.62	2.06	–	1.02
	A32	1 475	α-Himachalene	–	–	–	–	0.53
	A33	1 494	(+)-β-瑟林烯 (+)-β-Selinene	3.76	2.67	0.54	1.87	3.01
	A34	1 489	右旋大根香叶烯 (+)- Germacrene D	6.50	3.70	9.41	2.29	6.13
	A35	1 498	愈创木烯Guaiacene	3.09	1.58	0.14	0.70	1.19
	A36	1 413	(+)-γ-古芸烯 (+)-γ-Gurjunene	–	–	–	–	3.71
	A37	1 428	(-)-β-花柏烯 (-)-β-Chamigrene	–	–	–	–	2.50
	A38	1 435	γ-榄香烯γ-Elemene	–	–	–	–	0.67
	A39	1 505	1-ethenyl-1-methyl-2-(1-methylethenyl)-4-(1-methylethylidene)-cyclohexane	5.09	7.03	6.42	0.42	6.43
	A40	1 510	(+)-香橙烯 (+)-Aromadendrene	0.66	0.67	1.00	0.13	–
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	相对含量/% Relative content
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	浙江楠 P. chekian- gensis	小叶桢楠 P. zhennan f. oblong	大叶桢楠 P. zhennan f. elliptical	紫楠 P. sheareri	闽楠 P. bour- nei
	A41	1 490	-Azulene,1,2,3,4,5,6,7,8-octahydro-1,4-dimethyl-7-(1-methylethylidene)-, (1S,4S)-	–	0.13	–	0.70	–
	A42	1 523	(+)-γ-荜澄茄烯 (+)-γ-Cadinene	–	–	–	–	0.53
	A43	1 533	δ-荜澄茄烯 δ-Cadinene	6.01	1.87	3.57	1.12	1.81
	A44	1 609	α-广藿香烯 α-Patchoulene	0.13	0.41	0.11	0.25	0.42
	A45	1 621	环氧化蛇麻烯Ⅱ Humulene1,2-epoxide	0.18	0.27	0.14	0.55	–
醛类 Aldehydes	B1	1 645	2-甲基-4-戊醛 2-Methylpent-4-enal	1.49	0.69	0.25	0.10	0.51
	B2	1 124	壬醛 Nonanal	0.13	–	–	–	0.12
	B3	1 210	癸醛 Decanal	0.17	0.35	0.27	–	–
	B4	1 305	10-十一烯醛10-Undecenal	–	–	–	–	0.13
	B5	1 310	十一醛 Undecanal	–	0.33	–	–	0.13
	B6	1 412	十二醛 Lauryl aldehyde	0.16	1.02	0.13	–	–
	B7	1 520	十四烷醛 Tetradecanal	–	–	–	0.17	–
醇类 Alcohols	C1	2 510	反式-3-己烯-1-醇 Trans-3-Hexen-1-ol	2.08	1.57	1.02	1.51	1.50
	C2	2 738	3-甲基-4-戊醇 3-Methylpent-4-en-1-ol	0.22	0.43	–	0.28	–
	C3	1 409	环十二醇Cyclododecanol	–	–	–	–	0.39
	C4	2 714	叶醇cis-3-Hexen-1-ol	0.17	–	0.36	–	–
萜烯醇类	D1	1 035	桉叶油醇 Cineole	0.10	–	–	3.97	–
Terpene alcohols	D2	1 108	芳樟醇 Linalool	0.43	–	–	–	–
	D3	1 094	橙花醇 Nerol	–	–	–	–	0.14
	D4	1 295	紫苏醇 Perillyl alcohol	–	–	–	–	0.13
	D5	1 540	α-檀香醇 α-Santalol	–	0.13	0.11	–	–
	D6	1 526	α-人参烯 α-Panasinsanene	0.11	–	–	–	–
	D7	1 584	4(15),5,10(14)-大根香叶三烯-1-醇 4(15),5,10(14)-Germacratrien-1-ol	–	0.14	0.13	–	–
	D8	1 590	桉油烯醇 Spathulenol	–	0.68	0.22	–	0.47
	D9	1 633	Β-桉叶醇 β-Eudesmol	0.16	0.10	–	–	–
	D10	1 650	1aS,4aS,7R,7aS,7bS)-1,1,7- Trimethyl-4-methylidene-1a,2,3, 4a,5,6,7a,7b-octahydrocyclopropa [h]azulen-7-ol	–	0.11	–	–	–
酯类 Esters	E1	1 027	(E)-3-己烯-1-醇乙酸酯 Trans-3-hexenyl acetate	0.26	0.21	0.54	0.49	3.57
	E2	1 028	乙酸叶醇酯 cis-3-Hexenyl acetate	–	0.10	–	–	–
萜烯酯类	F1	1 265	乙酸芳樟酯 Linalyl acetate	0.23	0.41	0.17	–	0.91
Terpene esters	F2	1 299	乙酸龙脑酯 Bornyl acetate	3.70	0.98	0.62	5.10	–
	F3	1 292	左旋乙酸冰片酯 L-Bornyl acetate	–	–	–	–	2.24
萘类 Naphthalenes	G1	1 517	(1aR)-1aβ,2,3,3a,4,5,6,7bβ- Octahydro-1,1,3aβ,7-tetramethyl- 1H-cyclopropa[a]naphthalene	1.85	0.89	1.59	0.35	–
	G2	1 542	1,2,3,4,4a,7-Hexahydro-1,6- dimethyl-4-(1-methylethyl)- naphthalene	0.35	0.30	0.29	–	0.20
	G3	1 548	1,2,4a,5,6,8a-Hexahydro-4,7- dimethyl-1-(1-methylethyl) napthtalene	0.15	0.14	0.24	0.11	0.41
	G4	1 578	(8aα)-Decahydro-1α-isopropyl- 3aα-methyl-7-methylene-4α,8α- epoxyazulene	–	0.19	0.10	–	0.42
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	相对含量/% Relative content
类别 Class	编号 No.	保留指数 Retention index	化合物 Compounds	浙江楠 P. chekian- gensis	小叶桢楠 P. zhennan f. oblong	大叶桢楠 P. zhennan f. elliptical	紫楠 P. sheareri	闽楠 P. bour- nei
烷烃类 Alkanes	H1	1 303	正十三烷Tridecane	–	–	–	0.10	0.13
	H2	1 308	环己烷Cyclohexane	–	–	–	–	0.67
	H3	1 556	(E)-2-(6,10-Timethylundeca-1,5,9- trien-2-yl)oxirane	0.11	–	–	–	–
有机酸 Organic acid	I1	1 283	(1R,4S)-1,7,7-Trimethylbicyclo [2.2.1]heptan-2-yl acetate	–	–	–	–	0.33
氧化物 Oxide	J1	1 595	石竹素Caryophyllene oxide	0.49	0.34	0.22	0.21	0.36

化合物 Compounds	相对含量/ % Relative content		化合物 Compounds	相对含量/% Relative content
化合物 Compounds	夏季 Summer	秋季 Autumn	化合物 Compounds	夏季 Summer	秋季 Autumn
莰烯Camphene	4.85 ± 2.09^**	1.03 ± 0.13	α-葎草烯 α-Humulene	1.61 ± 0.18	1.42 ± 0.04
甘香烯Caryophyllene	5.50 ± 1.67	3.20 ± 1.87	(-)-α-荜澄茄油烯 (-)-α-Cubebene	2.46 ± 0.03	1.76 ± 0.18
α-古巴烯α-Copaene	7.49 ± 2.09^*	6.30 ± 1.46	右旋大根香叶烯 (+)-Germacrene D	5.57 ± 2.23	5.52 ± 2.58
β-榄香烯β-Elemene	8.81 ± 2.97	8.91 ± 1.53	(-)-α-古芸烯 (-)-α-Gurjunene	4.10 ± 2.17^*	2.49 ± 1.62
β-石竹烯β-Caryophyllene	11.87 ± 2.31^*	8.92 ± 1.68	δ-荜澄茄烯 δ-Cadinene	6.44 ± 2.97^**	3.22 ± 1.93
马兜铃烯Aristolene	2.44 ± 0.18	2.32 ± 0.32	芹子烯 Apigenine	2.26 ± 2.05	2.87 ± 2.55
α-蒎烯α-Pinene	0.49 ± 0.05^**	4.40 ± 0.63	佛术烯 Eremophilene	1.69 ± 0.06	1.79 ± 0.40

化合物 Compounds	相对含量/ % Relative content		化合物 Compounds	相对含量/% Relative content
化合物 Compounds	夏季 Summer	秋季 Autumn	化合物 Compounds	夏季 Summer	秋季 Autumn
莰烯Camphene	4.85 ± 2.09^**	1.03 ± 0.13	α-葎草烯 α-Humulene	1.61 ± 0.18	1.42 ± 0.04
甘香烯Caryophyllene	5.50 ± 1.67	3.20 ± 1.87	(-)-α-荜澄茄油烯 (-)-α-Cubebene	2.46 ± 0.03	1.76 ± 0.18
α-古巴烯α-Copaene	7.49 ± 2.09^*	6.30 ± 1.46	右旋大根香叶烯 (+)-Germacrene D	5.57 ± 2.23	5.52 ± 2.58
β-榄香烯β-Elemene	8.81 ± 2.97	8.91 ± 1.53	(-)-α-古芸烯 (-)-α-Gurjunene	4.10 ± 2.17^*	2.49 ± 1.62
β-石竹烯β-Caryophyllene	11.87 ± 2.31^*	8.92 ± 1.68	δ-荜澄茄烯 δ-Cadinene	6.44 ± 2.97^**	3.22 ± 1.93
马兜铃烯Aristolene	2.44 ± 0.18	2.32 ± 0.32	芹子烯 Apigenine	2.26 ± 2.05	2.87 ± 2.55
α-蒎烯α-Pinene	0.49 ± 0.05^**	4.40 ± 0.63	佛术烯 Eremophilene	1.69 ± 0.06	1.79 ± 0.40

化合物 Compounds	相对含量/% Relative content
化合物 Compounds	15 ℃	25 ℃	35 ℃	45 ℃
蒎烯 Pinene	7.19 ± 1.43 a	5.86 ± 1.83 a	6.38 ± 1.89 a	6.30 ± 1.41 a
α-古巴烯 α-Copaene	4.22 ± 0.19 a	3.34 ± 0.73 a	2.70 ± 0.19 ab	2.36 ± 0.01 b
β-蒎烯 β-Pinene	3.66 ± 0.96 a	3.22 ± 0.13 a	3.00 ± 0.76 a	2.79 ± 1.10 a
(-)-异喇叭烯 (-)-isoledene	1.10 ± 0.11 a	1.15 ± 0.04 a	1.17 ± 0.11 a	1.20 ± 0.05 a
(-)-α-荜澄茄油烯 (-)-α-Cubebene	3.41 ± 0.29 a	2.98 ± 0.47 ab	2.44 ± 1.21 ab	2.08 ± 0.11 b
β-榄香烯 β-Elemene	5.19 ± 1.27 a	5.29 ± 0.94 a	5.46 ± 1.16 a	6.90 ± 1.04 a
β-石竹烯 β-Caryophyllene	18.82 ± 0.83 b	22.97 ± 0.18 a	20.66 ± 1.85 b	19.95 ± 1.98 b
(1S,4aR,8aR)-7-Isopropylidene-1,4a-dimethyldecahydro-1-naphthalen ol	1.44 ± 1.09 a	1.97 ± 0.32 a	1.60 ± 0.38 a	1.94 ± 0.52 a
马兜铃烯 Aristolene	1.13 ± 0.11 a	1.35 ± 0.16 a	1.13 ± 0.11 a	0.89 ± 0.13 a
α-葎草烯 α-Humulene	16.40 ± 0.29 b	17.98 ± 0.48 ab	19.83 ± 0.36 a	18.91 ± 1.53 a
(+)-β-瑟林烯 (+)-β-Selinene	3.41 ± 0.12 a	3.41 ± 0.79 a	2.59 ± 0.45 b	3.39 ± 0.19 a
α-瑟林烯 α-Selinene	3.90 ± 0.14 b	4.29 ± 0.11 b	4.77 ± 0.35 b	6.61 ± 0.49 a
(+)-γ-古芸烯 (+)-γ-Gurjunene	5.04 ± 0.08 a	5.14 ± 1.18 a	3.22 ± 0.17 b	4.48 ± 0.17 ab
乙酸龙脑酯 Bornyl acetate	1.04 ± 0.04 a	0.97 ± 0.02 a	1.01 ± 0.01 a	0.87 ± 0.02 a
α-法呢烯 α- Farnesene	2.13 ± 0.56 a	1.30 ± 0.57 b	1.13 ± 0.12 b	0.71 ± 0.12 b
1-ethenyl-1-methyl-4-propan-2-ylidene-2-prop-1-en-2-ylcyclohexane	3.15 ± 1.82 a	2.51 ± 0.51 a	3.46 ± 0.37 a	2.24 ± 0.46 a

Determination of Leaf Volatile Components from Five Phoebe Species

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化合物 Compounds	相对含量/% Relative contents/%
化合物 Compounds	8：00	10：00	12：00	14：00	16：00	18：00
右旋大根香叶烯(+)-Germacrene D	9.79	13.00	7.72	8.57	15.64	10.39
β-石竹烯 β-Caryophyllene	12.53	12.85	10.56	13.92	13.08	13.38
α-古巴烯 α-Copaene	5.87	4.95	4.67	7.98	4.05	6.49
β-荜澄茄烯 β-Cadinene	0.92	1.00	0.52	0.89	0.96	1.02
γ-摩勒烯 γ-muurolene	2.19	2.45	4.72	3.00	2.28	2.38
β-榄香烯 β-Elemene	6.95	8.40	5.87	8.58	8.44	7.74
叶醇cis-3-Hexen-1-ol	—	—	—	3.07	—	—
桉油烯醇 Spathulenol	0.17	0.10	0.19	0.13	—	—
α-葎草烯 α-Humulene	1.65	1.95	2.02	1.71	1.99	1.82

[1]	QIANG Wenyan, MENG Qingran, ZHANG Zhiguo, GAO Wenjie. Analysis of Petal Volatile Components Among Different Hemerocallis Cultivars Based on HS-SPME-GC-MS [J]. Acta Horticulturae Sinica, 2023, 50(1): 116-130.
[2]	ZHU Linlin, ZHOU Wanfei, NING Xuan, ZHU Jun, XI Wan, ZENG Xumei, XIONG Kangshun, WANG Caiyun, LUO Jing, ZHENG Riru. Analysis of Free Aromatic Components of Different Osmanthus fragrans Cultivars [J]. Acta Horticulturae Sinica, 2022, 49(11): 2395-2406.
[3]	WANG Junwen, WU Yue, YU Jihua, ZHANG Jing, XIE Jianming, FENG Zhi, TANG Zhongqi, LIU Xiaoqi, LI Jing, ZHONG Yuan. Soluble Sugar,Organic Acid Quality and Volatile Compounds Contents in Tomato Fruits can be Promoted by Exogenous ALA [J]. Acta Horticulturae Sinica, 2021, 48(5): 973-986.
[4]	SHEN Zhiguo, SUN Meng, YUAN Deyi, CHENG Jianming, DING Xin, and SHANG Zhonghai, . HS–SPME–GC–MS Analysis of Volatile Components in Tender Shoots from Six Plants of Calycanthaceae [J]. Acta Horticulturae Sinica, 2020, 47(12): 2349-2361.
[5]	YAO Chenyang1,2，GE Hong2，WU Hua3，JIA Ruidong2，ZHAO Xin2，Lü Yingmin1,，and YANG Shuhua2,. Petal Volatile Components Among Different Varieties of Rosa rugosa [J]. ACTA HORTICULTURAE SINICA, 2019, 46(2): 375-384.
[6]	WANG Hua-lei;FENG Jian-rong;FAN Xin-min;and HE Feng-jiang. Study on Volatile Constituents in Mainly Cultivated Apricot Native to Xinjiang [J]. ACTA HORTICULTURAE SINICA, 2009, 36(7): 1043-1048.
[7]	LIU Chong-qi;CHEN Xue-sen;WANG Jin-zheng;CHEN Xiao-liu;WANG Hai-bo;TIAN Chang-ping _;and WU Chuan-jin . Studies on genetic diversity of phenotypic traits in wild myrobalan plum(Prunus cerasifera Ehrh.) [J]. ACTA HORTICULTURAE SINICA, 2008, 35(9): 1261-1268.
[8]	ZHU Hong;CHEN Yu-fen;LI Xue-ping;LI Jun;HAN Dong-fang;and CHEN Wei-xin. Determ ina tion of Volatiles in Harvested Banana Fruit by HS-SPME andGC-MS [J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 485-488.
[9]	ZHANG Ling-zhi;WANG Deng-liang;CHEN Wei-xin;and CHEN Yu-fen. Determination of Volatiles of Puer Tea Stored for Different Lengths of Time [J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 504-506.
[10]	Li Ming;Wang Liping;Zhang Yang;Wang Jianxin. Solid Phase Microextraction- GC-MS Analysis of Fruit Aroma Components of Peach Cultivars [J]. ACTA HORTICULTURAE SINICA, 2006, 33(5): 1071-1074.
[11]	Chen Meixia;Chen Xuesen;Feng Baochun. GC2MS Analysis of Fruit Aroma Components of Two Apricot Cultivars [J]. ACTA HORTICULTURAE SINICA, 2004, 31(5): 663-665.
[12]	Zhang Ji;Ma Junyi;Huang Ailun;Yao Jian;and Yang Yongli. Studies on the Essential Constituent of Rhododendron thymifolium Maxim. [J]. ACTA HORTICULTURAE SINICA, 2002, 29(4): 386-388.