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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (6): 1094-1106.doi: 10.16420/j.issn.0513-353x.2020-0542

• Research Papers • Previous Articles     Next Articles

Development and Application of KASP Markers for Clubroot Resistance Gene CRs in Chinese Cabbage

YANG Shuangjuan, YUAN Yuxiang, ZHAO Yanyan, WEI Xiaochun, WANG Zhiyong, ZHAO Xiaobin, ZHANG Xiaowei()   

  1. Institute of Horticulture,Henan Academy of Agricultural Sciences,Zhengzhou 450002,China
  • Received:2020-12-18 Revised:2021-02-17 Online:2021-06-25 Published:2021-07-07
  • Contact: ZHANG Xiaowei E-mail:xiaowei5737@163.com

Abstract:

CRs is an recently discovered clubroot resistance gene in Chinese cabbage. In this study,through PCR amplication and sequencing of four CRs linked markers in eight resistant materials and eight susceptible materials,we found six sequence variations between resistant and susceptible materials. Among which,five variations(9 956 808,9 956 888,9 956 904,10 707 299 and 10 707 385 bp in chromosome A08)were novel,G/A SNP(Single Nucleotide Polymorphisms)variation in 10 539 495 bp of A08 is consistent with previous study. Five KASP(Kompetitive allele specific PCR)markers, Probe55K1,Probe55K2,Probe64K1,Probe64K2 and Probe60K1,were developed in terms of variations in 9 956 808,9 956 888,10 707 299,10 707 385 and 10 539 495 bp. It turned out that all the five KASP markers can successfully differentiate the resistance from the susceptible materials. Probe55K1 and Probe64K1 were further genotyped in 45 resistant and 48 susceptible individuals, which were part of a BC1P1 population. The results showed that the consistency rate between genotypes and phenotypes of the above 93 individuals was high to 94.62%. Thus,the KASP markers developed in this study can be efficently used for marker-assisted selection(MAS)of clubroot resistant gene in breeding.

Key words: Chinese cabbage, clubroot, CRs gene, KASP marker

CLC Number: