Advances on Fertility Restoration for Cytoplasmic Male Sterility in Vegetables

doi:10.16420/j.issn.0513-353x.2020-0795

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (5): 1031-1046.doi: 10.16420/j.issn.0513-353x.2020-0795

• Reviews • Previous Articles Next Articles

Advances on Fertility Restoration for Cytoplasmic Male Sterility in Vegetables

YU Hailong, REN Wenjing, FANG Zhiyuan, YANG Limei, ZHUANG Mu, LÜ Honghao, WANG Yong, JI Jialei, ZHANG Yangyong^*()

Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

Received:2021-01-31 Revised:2021-03-25 Online:2021-05-25 Published:2021-06-07
Contact: ZHANG Yangyong E-mail:zhangyangyong@caas.cn

Abstract

Abstract:

In recent years,various types of cytoplasmic male sterile(CMS)lines and their restorer lines have been reported in vegetables,and a number of restorer genes have been located and cloned,and the markers closely linked with restorer genes have been developed and used in vegetable breeding. Here,recent progress on CMS restorer lines were reviewed,as well as the mapping and cloning of restorer genes,the marker development of restorer genes,and the application of restorer lines in breeding of vegetables. The existing problems and the development trend in the future were discussed.

Key words: vegetables, cytoplasmic male sterility, restorer gene, gene mapping, gene cloning, molecular marker

CLC Number:

YU Hailong, REN Wenjing, FANG Zhiyuan, YANG Limei, ZHUANG Mu, LÜ Honghao, WANG Yong, JI Jialei, ZHANG Yangyong. Advances on Fertility Restoration for Cytoplasmic Male Sterility in Vegetables[J]. Acta Horticulturae Sinica, 2021, 48(5): 1031-1046.

Figures/Tables 3

References 124

[1]	Alessandro M S, Galmarini C R, Iorizzo M, Simon P W. 2013. Molecular mapping of vernalization requirement and fertility restoration genes in carrot. Theor Appl Genet, 126(2):415-423. doi: 10.1007/s00122-012-1989-1 URL
[2]	Bang H, Cho DY, Yoo K, Yoon M, Patil B S, Kim S. 2011. Development of simple PCR-based markers linked to the Ms locus,a restorer-of-fertility gene in onion(Allium cepa L.). Euphytica, 179:439-449. doi: 10.1007/s10681-010-0342-5 URL
[3]	Bang H, Kim S, Park S O, Yoo K S, Patil B S. 2013. Development of a codominant CAPS marker linked to the Ms locus controlling fertility restoration in onion(Allium cepa L.). Sci Hortic, 153:42-49. doi: 10.1016/j.scienta.2013.01.020 URL
[4]	Bannerot H, Boulidard L, Couderon Y, Temple J. 1974. Transfer of cytoplasmic male sterility from Raphanus sativus to Brassica oleracea//Wills A B,North C. Proc Eucarpia Meet Cruciferae. Scottish Hortic Res Inst, Invergavrie:52-54.
[5]	Barchenger D W, Said J I, Zhang Y, Song M, Ortega F A, Ha Y, Kang B C, Bosland P W. 2018. Genome-wide identifcation of chile pepper pentatricopeptide repeat domains provides insight into fertility restoration. J Am Soc Hort Sci, 143(6):418-429. doi: 10.21273/JASHS04522-18 URL
[6]	Bellaoui M, Grelon M, Pelletier G, Budar F. 1999. The restorer Rfo gene acts post-translationally on the stability of the ORF138 Ogura CMS-associated protein in reproductive tissues of rapeseed cybrids. Plant Molecular Biology, 40(5):893-902. doi: 10.1023/A:1006223908044 URL
[7]	Bett K E, Lydiate D J. 2004. Mapping and genetic characterization of loci controlling the restoration of male fertility in Ogura CMS radish. Molecular Breeding, 13(2):125-133. doi: 10.1023/B:MOLB.0000018759.27223.02 URL
[8]	Brown G G, Formanova N, Jin H, Wargachuk R, Dendy C, Pati P, Lafores M, Zhang J, Cheung W Y, Landry B S. 2003. The radish Rfo restorer gene of Ogura cytoplasmic male sterility encodes a protein with multiple pentatricopeptide repeats. Plant Journal, 35(2):262-272. doi: 10.1046/j.1365-313X.2003.01799.x URL
[9]	Chen F, Song Y, Li X, Chen J, Mo L, Zhang X, Lin Z, Zhang L. 2019. Genome sequences of horticultural plants:past,present,and future. Horticulture research, 6(1):1-23. doi: 10.1038/s41438-018-0066-6 URL
[10]	Chen L T, Liu Y G. 2014. Male sterility and fertility restoration in crops. Annual Review of Plant Biology, 65:579-606. doi: 10.1146/annurev-arplant-050213-040119 URL
[11]	Cheng J, Chen Y, Hu Y, Zhou Z, Hu F, Dong J, Chen W, Cui J, Wu Z, Hu K. 2020. Fine mapping of restorer-of-fertility gene based on high-density genetic mapping and collinearity analysis in pepper(Capsicum annuum L.). Theor Appl Genet, 133(3):889-902. doi: 10.1007/s00122-019-03513-y URL
[12]	Cho Y, Lee Y, Park B, Han T, Kim S. 2012. Construction of a high-resolution linkage map ofRfd1,a restorer-of-fertility locus for cytoplasmic male sterility conferred by DCGMS cytoplasm in radish(Raphanus sativus L.)using synteny between radish and Arabidopsis genomes. Theor Appl Genet, 125:467-477. doi: 10.1007/s00122-012-1846-2 URL
[13]	Cui X Q, Wise R P, Schnable P S. 1996. The rf2 nuclear restorer gene of male-sterile T-cytoplasm maize. Science, 272(5266):1334-1336. doi: 10.1126/science.272.5266.1334 URL
[14]	Delourme R, Bouchereau A, Hubert N, Renard M, Landry B S. 1994. Identification of RAPD markers linked to a fertility restorer gene for the Ogura radish cytoplasmic male sterility of rapeseed(Brassica napus L.). Theor Appl Genet, 88:741-748. doi: 10.1007/BF01253979 pmid: 24186171
[15]	Delourme R, Eber F. 1992. Linkage between an isozyme marker and a restorer gene in radish cytoplasmic male sterility of rapeseed(Brassica napus L.). Theor Appl Genet, 85:222-228. doi: 10.1007/BF00222863 pmid: 24197308
[16]	Desloire S, Gherbi H, Laloui W, Marhadour S, Clouet V, Cattolico L, Falentin C, Giancola S, Renard M, Budar F, Small I, Caboche M, Delourme R, Bendahmane A. 2003. Identification of the fertility restoration locus Rfo in radish,as a member of the pentatricopeptide-repeat protein family. EMBO Reports, 4(6):588-594. doi: 10.1038/sj.embor.embor848 URL
[17]	Fang Muren, Mao Ruichang, Xie Wenhua. 1985. Breeding of cytoplasmivally inherited male sterile lines of eggplant(Solanum melongena L.) . Acta Horticulturae Sinica,(4):261-266. (in Chinese)
	方木壬, 毛瑞昌, 谢文华. 1985. 茄子胞质雄性不育系的选育. 园艺学报,(4):261-266.
[18]	Ferreira R R, Santos C A F, Oliveira V R. 2017. Fertility restoration locus and cytoplasm types in onion. Genet Mol Res, 16:1-12.
[19]	Geng Sansheng, Chen Bin, Zhang Xiaofen, Mao Aijun. 2005. A new hot pepper F₁ hybrid-‘Jingla No. 2’ . China Vegetables,(Z1):48-49. (in Chinese)
	耿三省, 陈斌, 张晓芬, 毛爱军. 2005. 利用雄性不育选育辣椒新品种京辣2号. 中国蔬菜,(Z1):48-49.
[20]	Gokce A F, Havey M J. 2002. Linkage equilibrium among tightly linked RFLPs and the Ms locus in open-pollinated onion populations. J Am Soc Hortic Sci, 127:944-946.
[21]	Gulyas G, Pakozdi K, Lee J S, Hirata Y. 2006. Analysis of fertility restoration by using cytoplasmic male-sterile red pepper(Capsicum annuum L.)lines. Breed Sci, 56:331-334. doi: 10.1270/jsbbs.56.331 URL
[22]	Havey M J. 1999. Seed yield,floral morphology,and lack of male-fertility restoration of male-sterile onion (Allium cepa) populations possessing the cytoplasm ofAllium galanthum. J Am Soc Hortic Sci, 124(6):626-629.
[23]	He Qiwei, Shi Huilian, An Zhenqin, Lang Fengqing, Zhao Shuangyi, Zhang Yanjun, Su Yiwen, Zhou Tongdu. 1993. Study on inheritance of male sterility in Chinese radish. Shandong Agricultural Sciences,(4):5-8. (in Chinese)
	何启伟, 石惠莲, 安振琴, 郎丰庆, 赵双宜, 张燕君, 粟翼玟, 周同度. 1993. 中国萝卜雄性不育性遗传规律的研究. 山东农业科学,(4):5-8.
[24]	He Qiwei, Shi Huilian. 1987. Studies on the breeding and inheritance of male sterility line of Chinese radish. Scientia Agricultura Sinica,(2):26-33. (in Chinese)
	何启伟, 石惠莲. 1987. 萝卜雄性不育系选育及遗传机制的研究. 中国农业科学,(2):26-33.
[25]	He S, Lyznik A, Mackenzie S. 1995. Pollen fertility restoration by nuclear gene Fr in CMS bean:nuclear-directed alteration of a mitochondrial population. Genetics, 139(2):955-962. pmid: 7713444
[26]	Huang Zhen, Chang Shaodong, Xia Xiuxian, Zhang Suping, Cao Cuiwen, Zou Jiwen. 2006. A new hot pepper F₁ hybrid-‘Layou No. 8’ . Guangdong Agricultural Sciences,(12):34-36. (in Chinese)
	黄贞, 常绍东, 夏秀娴, 张素平, 曹翠文, 邹集文. 2006. 辣椒新品种辣优8号的选育. 广东农业科学,(12):34-36.
[27]	Huo Y M, Liu B J, Yang Y Y, Miao J, Gao L M, Kong S P, Wang Z B, Kitano H, Wu X. 2015. AcSKP1,a multiplex PCR-based co-dominant marker in complete linkage disequilibrium with the male-fertility restoration(Ms)locus,and its application in open-pollinated populations of onion. Euphytica, 204(3):711-722. doi: 10.1007/s10681-015-1374-7 URL
[28]	Huo Y M, Miao J, Liu B J, Yang Y Y, Zhang Y H, Tahara Y, Meng Q W, He Q W, Kitano H, Wu X. 2012. The expression of pectin methylesterase in onion flower buds is associated with the dominant male-fertility restoration allele. Plant Breed, 131:211-216. doi: 10.1111/pbr.2011.131.issue-1 URL
[29]	Janska H, Sarria R, Woloszynska M, Arrietamontiel M, Mackenzie S A. 1998. Stoichiometric shifts in the common bean mitochondrial genome leading to male sterility and spontaneous reversion to fertility. The Plant Cell, 10:1163-1180. pmid: 9668135
[30]	Jia M H, He S, Vanhouten W, Mackenzie S. 1997. Nuclear fertility restorer genes map to the same linkage group in cytoplasmic male-sterile bean. Theor Appl Genet, 95(1-2):205-210. doi: 10.1007/s001220050549 URL
[31]	Jindal S K, Dhaliwal M S, Meena O P. 2020. Molecular advancements in male sterility systems ofCapsicum:a review. Plant Breeding, 139(1):42-64. doi: 10.1111/pbr.v139.1 URL
[32]	Jo Y D, Ha Y, Lee J H, Park M, Bergsma A C, Choi H I, Gortischnig S, Kloosterman B, van Dijk P J, Choi D, Kang B C. 2016. Fine mapping of restorer-of-fertility in pepper(Capsicum annuum L.)identifed a candidate gene encoding a pentatricopeptide repeat(PPR)-containing protein. Theor Appl Genet, 129:2003-2017. doi: 10.1007/s00122-016-2755-6 URL
[33]	Jo Y D, Kim Y M, Park M N, Yoo J H, Park M, Kim B D, Kang B C. 2010. Development and evaluation of broadly applicable markers for restorer-of-fertility in pepper. Molecular Breeding, 25:187-201. doi: 10.1007/s11032-009-9318-3 URL
[34]	Jones H A, Clarke A. 1943. Inheritance of male sterility in the onion and the production of hybrid seed. Proc Am Soc Hortic Sci, 43:189-194.
[35]	Ke Guilan, Zhang Lugang. 1993. Study on the relationship of restoration and maintenance of alloplasmic male sterile in Chinese cabbage. Acta Agriculturae Boreali-occidentalis Sinica, 2(1):15-20. (in Chinese)
	柯桂兰, 张鲁刚. 1993. 大白菜异源胞质雄性不育恢保关系的研究. 西北农业学报, 2(1):15-20.
[36]	Ke Guilan, Zhao Zhiya, Song Yanzhi, Zhang Lugang, Zhao Limin. 1992. Breeding of alloplasmic male sterile line CMS3411-7 in Chinese cabbage [Brassica campestris L. ssp. pekinensis(lour)olsson] and its application . Acta Horticulturae Sinica, 19(4):333-340,388. (in Chinese)
	柯桂兰, 赵稚雅, 宋胭脂, 张鲁刚, 赵利民. 1992. 大白菜异源胞质雄性不育系CMS3411-7的选育及应用. 园艺学报, 19(4):333-340,388.
[37]	Khan M M R, Hasnunnahar M, Iwayoshi M, Isshiki S. 2014. Fertility restoration in three CMS systems of eggplant by the Rf genes of each other’s systems and their SCAR marker. Sci Hortic, 172:149-154. doi: 10.1016/j.scienta.2014.04.013 URL
[38]	Khan M M R, Isshiki S. 2010. Development of the malesterile line of eggplant utilizing the cytoplasm of Solanum aethiopicum L. Aculeatum Group. J. Japan. Soc Hort Sci, 79:348-353. doi: 10.2503/jjshs1.79.348 URL
[39]	Khan M M R, Isshiki S. 2011. Development of a cytoplasmic male-sterile line of eggplant(Solanum melongena L.)with the cytoplasm of Solanum anguivi. Plant Breed, 130:256-260. doi: 10.1111/pbr.2011.130.issue-2 URL
[40]	Khan M M R, Iwayosh M, Ogura-tsujita Y, Isshiki S. 2018. Genetic characterization of the fertility restorer(Rf)genes and their linked DNA marker of the male sterile eggplant systems having the cytoplasm of the wild species. Environmental Control in Biology, 56(4):173-176. doi: 10.2525/ecb.56.173 URL
[41]	Kim D S, Kim D H, Yoo J H, Kim B D. 2006. Cleaved amplified polymorphic sequence and amplified fragment length polymorphism markers linked to the fertility restorer gene in chili pepper( Capsicum annuum L.). Molecules and Cells, 21:135-140.
[42]	Kim K, Lee Y P, Lim H, Han T, Sung S K, Kim S. 2010. Identification of Rfd1,a novel restorer-of-fertility locus for cytoplasmic male-sterility caused by DCGMS cytoplasm and development of simple PCR markers linked to theRfd1 locus in radish(Raphanus sativus L.). Euphytica, 175:79-90. doi: 10.1007/s10681-010-0190-3 URL
[43]	Kim S, Kim C W, Park M, Choi D. 2015. Identification of candidate genes associated with fertility restoration of cytoplasmic male-sterility in onion(Allium cepa L.)using a combination of bulked segregant analysis and RNA-seq. Theor Appl Genet, 128(11):2289-2299. doi: 10.1007/s00122-015-2584-z URL
[44]	Kim S. 2014. A codominant molecular marker in linkage disequilibrium with a restorer-of-fertility gene(Ms)and its application in reevaluation of inheritance of fertility restoration in onions. Mol Breed, 34:769-778. doi: 10.1007/s11032-014-0073-8 URL
[45]	Kim Y J, Zhang D. 2018. Molecular control of male fertility for crop hybrid breeding. Trends in Plant Science, 23(1):53-65. doi: 10.1016/j.tplants.2017.10.001 URL
[46]	King J J, Bradeen J M, Bark O, McCallum J A, Havey M J. 1998. A low-density genetic map of onion reveals a role for tandem duplication in the evolution of an extremely large diploid genome. Theor Appl Genet, 96:52-62. doi: 10.1007/s001220050708 URL
[47]	Kumar S, Banerjee M K, Kalloo G. 2000. Male sterility:mechanisms and current status on identification,characterization and utilization in vegetables. Vegetable Science, 27(1):1-24.
[48]	Kumar S, Singh V, Singh M, Rai S, Kumar S, Rai S K, Rai M. 2007. Genetics and distribution of fertility restoration associated RAPD markers in inbreds of pepper(Capsicum annuum L.). Scientia Horticulturae, 111:197-202. doi: 10.1016/j.scienta.2006.10.021 URL
[49]	Lee J, Yoon J B, Park H G. 2008a. A CAPS marker associated with the partial restoration of cytoplasmic male sterility in chili pepper(Capsicum annuum L.). Molecular Breeding, 21:95-104. doi: 10.1007/s11032-007-9111-0 URL
[50]	Lee Y P, Cho Y, Kim S. 2014. A high-resolution linkage map of the Rfd1,a restorer-of-fertility locus for cytoplasmic male sterility in radish(Raphanus sativus L.)produced by a combination of bulked segregant analysis and RNA-Seq. Theor Appl Genet, 127(10):2243-2252. doi: 10.1007/s00122-014-2376-x URL
[51]	Lee Y, Park S, Lim C, Kim H, Lim H, Ahn Y, Sung S, Yoon M, Kim S. 2008b. Discovery of a novel cytoplasmic male-sterility and its restorer lines in radish(Raphanus sativus L.). Theor Appl Genet, 117:905-913. doi: 10.1007/s00122-008-0830-3 URL
[52]	Lin Duo, Fu Xiang, Yang Yanjie, Ma Jing, Wang Hui, Zhu Wenying. 2020. A method for breeding male sterile lines and homozygous restoring gene lines in pepper by using linkage markers of restoring gene. China:CN110129481B,2020-04-14.
	林多, 付翔, 杨延杰, 马静, 王辉, 朱文莹. 2020. 一种利用恢复基因连锁标记同时选育辣椒雄性不育系和纯合恢复基因株系的方法. 中国:CN110129481B,2020-04-14.
[53]	Lin S W, Shieh H C, Wang Y W, Tan C W, Schafeitner R, Yang W J, Kumar S. 2015. Restorer breeding in sweet pepper:introgressing Rf allele from hot pepper through marker-assisted backcrossing. Scientia Hort, 197:170-175. doi: 10.1016/j.scienta.2015.09.036 URL
[54]	Liu F, Cui X, Horner H T, Weiner H, Schnable P S. 2001. Mitochondrial aldehyde dehydrogenase activity is required for male fertility in maize. Plant Cell, 13:1063-1078. pmid: 11340182
[55]	Liu Jinbing, Wang Shubin, Pan Baogui. 2004. A new sweet pepper F₁ hybrid-‘Jiangshu No. 5’ . China Vegetables,(3):26-27. (in Chinese)
	刘金兵, 王述彬, 潘宝贵. 2004. CMS三系配套品种江蔬5号甜椒的选育. 中国蔬菜,(3):26-27.
[56]	Liu Jinbing, Zhao Hualun, Wang Shubin, Pan Baogui, Sun Jiebo, Ding Liping. 2002. Creation and heterosis utilization of cytoplasmic male sterile restoring lines in sweet pepper. Journal of China Capsicum,(2):23-25. (in Chinese)
	刘金兵, 赵华仑, 王述彬, 潘宝贵, 孙洁波, 丁犁平. 2002. 甜椒CMS恢复系的创造及其杂种优势利用. 中国辣椒,(2):23-25.
[57]	Liu Z, Yang Z H, Wang X, Li K D, An H, Liu J, Yang G S, Fu T D, Yi B, Hong D F. 2016. A mitochondria-targeted PPR protein restores pol cytoplasmic male sterility by reducingorf224 transcript levels in oilseed rape. Molecular Plant, 9(7):1082-1084. doi: 10.1016/j.molp.2016.04.004 URL
[58]	Lv J H, Liu Z B, Liu Y H, Ou L J, Deng M H, Wang J, Song J S, Ma Y Q, Chen W C, Zhang Z Q, Dai X Z, Zou X X. 2020. Comparative transcriptome analysis between cytoplasmic male-sterile line and its maintainer during the floral bud development of pepper. Horticultural Plant Journal, 6(2):89-98. doi: 10.1016/j.hpj.2020.01.004 URL
[59]	Mackenzie S A, Bassett M. 1987. Genetics of restoration in cytoplasmic male sterile Phaseolus vulgaris L. Theor Appl Genet, 74:642-645. doi: 10.1007/BF00288864 pmid: 24240221
[60]	Mackenzie S A, Chase C D. 1990. Fertility restoration is associated with loss of a portion of the mitochondrial genome in cytoplasmic male-sterile common bean. The Plant Cell, 2(9):905-912. pmid: 12354971
[61]	Martin W J, McCallum J, Shigyo M, Jakse J, Kuhl J C, Yamane N, Pither-Joyce M, Gokce A F, Sink K C, Town C D, Havey M J. 2005. Genetic mapping of expressed sequences in onion and in silico comparisons with rice show scant colinearity. Mol Genet Genomics, 274:197-204 doi: 10.1007/s00438-005-0007-6 URL
[62]	Melchers G, Mohri Y, Watanabe K, Wakabayashi S, Harada K. 1992. One step generation of cytoplasmic male sterility by fusion of mitochondrial inactivated protoplasts with nuclear inactivated Solanum protoplasts. Proc Nat Acad Sci, 89:6832-6836. doi: 10.1073/pnas.89.15.6832 URL
[63]	Min W K, Kim S, Sung S K, Kim B D, Lee S. 2009. Allelic discrimination of the restorer-of-fertility gene and its inheritance in peppers(Capsicum annuum L). Theor Appl Genet, 119:1289-1299. doi: 10.1007/s00122-009-1134-y URL
[64]	Min W K, Lim H, Lee Y P, Sung S K, Kim B D, Kim S. 2008. Identification of third haplotype of the sequence linked to the restorer of fertility(Rf)gene and its implications for male sterility phenotypes in peppers (Capsicum annuum L.). Molecules and Cells, 25:20-29.
[65]	Morelock T E. 1974. Influence of cytoplasmic source on expression of male sterility in carrot(Daucus carota L.)[Ph. D. Dissertation]. Madison:The University of Wisconsin.
[66]	Murayama S, Habuchi T, Yamagishi H, Terachi T. 2002. Identification of a sequence-tagged site(STS)marker linked to a restorer gene for Ogura cytoplasmic male sterility in radish(Raphanus sativus L.)by non-radioactive AFLP analysis. Euphytica, 129:61-68. doi: 10.1023/A:1021513331079 URL
[67]	Murayama S, Yamagishi H, Terachi T. 1999. Identidication of RAPD and SCAR markers linked to a restorer gene for Ogura cytoplasmic male sterility in radish(Raphanus sativus L.)by bulked segregant anlysis. Breed Sci, 49:115-121. doi: 10.1270/jsbbs.49.115 URL
[68]	Nahm S H, Lee H J, Lee S W, Joo G Y, Harn C H, Yang S G, Min B W. 2005. Development of a molecular marker specific to a novel CMS line in radish(Raphanus sativus L.). Theor Appl Genet, 111:1191-1200. doi: 10.1007/s00122-005-0052-x URL
[69]	Ogura H. 1968. Studies on the new male-sterility in Japanese radish,with special reference to the utilization of this sterility towards the practical raising of hybrid seeds. Mem Fac Agric Kagoshima Univ, 6:39-78.
[70]	Ortega F A, Barchenger D W, Wei B, Bosland P W. 2020. Development of a genotype-specific molecular marker associated with restoration-of-fertility(Rf)in chile pepper(Capsicum annuum). Euphytica, 216(3):1-10. doi: 10.1007/s10681-019-2539-6 URL
[71]	Park J, Bang H, Cho D Y, Yoon M, Patil B S, Kim S. 2013. Construction of high-resolution linkage map of the Ms locus,a restorer-of-fertility gene in onion(Allium cepa L.). Euphytica, 192:267-278. doi: 10.1007/s10681-012-0851-5 URL
[72]	Pearson O H. 1972. Cytoplasmically inherited male sterility characters and flavor components from the species cross Brassica nigra(L.)Koch × B. oleracea L. Amer Soc Hort Sci J, 97:397-402.
[73]	Peterson P A. 1958. Cytoplasmically inherited male sterility in Capsicum. The American Naturalist, 92:111-119.
[74]	Petrova M, Yulkova Z, Gorinova N, Izhar S, Firon N, Jacquemin J M, Atanassov A, Stoeva P. 1999. Characterization of a cytoplasmic male sterile hybrid between Lycopersicon peruvianum Mill. × Lycopersicon pennellii Corr. and its crosses with tomato. Theor Appl Genet, 98:39-48.
[75]	Qin X K, Warguchuk R, Arnal N, Gaborieau L, Mireau H, Brown G G. 2014. In vivo functional analysis of a nuclear restorer PPR protein. BMC Plant Biology, 14(1):313.
[76]	Ren W J, Li Z Y, Han F Q, Zhang B, Li X, Fang Z Y, Yang L M, Zhuang M, Lv H H, Liu Y M, Wang Y, Yu H L, Zhang Y Y. 2020. Utilization of Ogura CMS germplasm with the clubroot resistance gene by fertility restoration and cytoplasm replacement in Brassica oleracea L. Horticulture Research, 7(1):1-10.
[77]	Saito T, Matsunaga H, Saito A, Hamato N, Koga T, Suzuki T, Yoshida T. 2009. A novel source of cytoplasmic male sterility and a fertility restoration gene in eggplant(Solanum melongena L.) lines. J. Japan. Soc. Hort. Sci., 78:425-430.
[78]	Schnable P S, Wise R P. 1998. The molecular basis of cytoplasmic male sterility and fertility restoration. Trends in Plant Science, 3(5):175-180.
[79]	Schweisguth B. 1973. A new type of male-sterility in onion,Allium cepa L. Annales De L'amelioration Des Plantes, 23:221-233.
[80]	Shen Huolin, Jiang Jianzhen, Wang Zhiyuan, Geng Sansheng. 1994. Studies on the breeding and inheritance of male sterility lines of pepper(Capsicum annuum L.) . Acta Agriculturae Universitatis Pekinensis,(20):25-30. (in Chinese)
	沈火林, 蒋健箴, 王志源, 耿三省. 1994. 辣椒雄性不育系选育及遗传研究. 北京农业大学学报,(20):25-30.
[81]	Shifriss C, Frankel R. 1971. New sources of cytoplasmic male sterility in cultivated peppers. Journal of Heredity, 62:254-256.
[82]	Shifriss C, Guri A. 1979. Variation in stability of cytoplasmic male sterility in Capsicum annuum L. Journal of the American Society for Horticultural Science, 104:94-96.
[83]	Singh S, Dey S S, Bhatia R, Kumar R, Behera T K. 2019. Current understanding of male sterility systems in vegetable Brassicas and their exploitation in hybrid breeding. Plant Reproduction, 32:231-256.
[84]	Tang Dongying, Zou Xuexiao, Liu Zhimin, Ma Yanqing, Zhang Zhuqing, Dai Xiongze. 2004. Identification of rapid marker linked to a restorer gene for cytoplasmic male sterility in pepper. Journal of Hunan Agricultural University(Natural Sciences), 30(4):307-309. (in Chinese)
	唐冬英, 邹学校, 刘志敏, 马艳青, 张竹青, 戴雄泽. 2004. 辣椒胞质雄性不育恢复基因的RAPD标记. 湖南农业大学学报(自然科学版), 30(4):307-309.
[85]	Thompson D J. 1961. Studies on the inheritance of male sterility in the carrot(Daucus carota L. var. sativa). Proc Am Soc Hort Sci, 78:332-338.
[86]	Thompson K F. 1972. Cytoplasmic male sterility in oilseed rape. Heredity, 29:253-257.
[87]	Uyttewaal M, Arnal N, Quadrado M, Martin-Canadell A, Vrielynck N, Hiard S, Gherbi H, Bendahmane A, Budar F, Mireau H. 2008. Characterization of Raphanus sativus pentatricopeptide repeat proteins encoded by the fertility restorer locus for Ogura cytoplasmic male sterility. Plant Cell, 20(12):3331-3345.
[88]	Wang L H, Zhang B X, Lefebvre V, Huang S W, Daubeze A M, Palloix A. 2004. QTL analysis of fertility restoration in cytoplasmic male sterile pepper. Theor Appl Genet, 109:1058-1063.
[89]	Wang Shubin, Liu Jinbing, Pan Baogui. 2007. Inheritance and distribution of fertility restoring gene of cytoplasmic male sterile pepper. Acta Agriculturae Boreali-Sinica, 22(1):86-89. (in Chinese)
	王述彬, 刘金兵, 潘宝贵. 2007. 辣(甜)椒细胞质雄性不育恢复基因的遗传与分布. 华北农学报, 22(1):86-89.
[90]	Wang Y, Wang Q, Hao W, Li J, Qi M, Zhang L. 2020. Mitochondrial genome sequencing reveals orf463a may induce male sterility in NWB cytoplasm of radish. Genes, 11(1):74.
[91]	Wang Z W, Wang C D, Cai Q Z, Mei S Y, Gao L, Zhou Y, Wang T. 2017. Identification of promoter exchange at a male fertility restorer locus for cytoplasmic male sterility in radish(Raphanus sativus L.). Molecular Breeding, 37(6):82.
[92]	Wang Z W, Wang C D, Gao L, Mei S Y, Zhou Y, Xiang C P, Wang T. 2013. Heterozygous alleles restore male fertility to cytoplasmic male-sterile radish(Raphanus sativus L.):a case of overdominance. J Exp Bot, 64:2041-2048.
[93]	Wang Z W, Zhang Y, Xiang C, Mei S Y, Zhou Y, Chen G P, Wang T. 2008. A new fertility restorer locus linked closely to the Rfo locus for cytoplasmic male sterility in radish. Theor Appl Genet, 117(3):313-320.
[94]	Wolyn D J, Chahal A. 1998. Nuclear and cytoplasmic interactions for petaloid male-sterile accessions of wild carrot (Daucus carota L.). J Am Soc Hort Sci, 123(5):849-853.
[95]	Wu Xiong, Yang Yanyan, Huo Yumeng, Liu Bingjiang, Miu Jun, Zhang Yihui. 2013. SCAR marker closly linked to male sterility restorer gene Ms in onion and its application . China,CN102925431A,2013-02-13. (in Chinese)
	吴雄, 杨妍妍, 霍雨猛, 刘冰江, 缪军, 张一卉. 2013. 与洋葱雄性不育恢复基因Ms紧密连锁的SCAR标记及其应用. 中国:CN102925431A,2013-02-13.
[96]	Xu Wenling, Wang Shufen, Wang Cuihua, Liu Xianxian, Fu Weimin, He Qiwei, Zhao Shuangyi. 2013. Gene expression profiling analysis of pol CMS fertility-restorer genes in Chinese cabbage(Brassica rapa L. ssp. pekinensis) . Acta Horticulturae Sinica, 40(8):1475-1486. (in Chinese)
	徐文玲, 王淑芬, 王翠花, 刘贤娴, 付卫民, 何启伟, 赵双宜. 2013. 大白菜pol CMS育性恢复基因的表达分析. 园艺学报, 40(8):1475-1486.
[97]	Xu Xiaoyong. 2013. Expression analysis of male sterility related gene and mapping the restorer gene in CMS7311 of heading Chinese cabbage[Ph. D. Dissertation]. Yangling:Northwest A & F University. (in Chinese)
	许小勇. 2013. 大白菜CMS7311不育发生相关基因表达研究及育性恢复基因定位[博士论文]. 杨凌:西北农林科技大学.
[98]	Xue Qinghua, Yang Fengmei, Yang Gaoqiang, Xue Yumei. 2014. Breeding of shenyan 20-a new three-lined hybrid sweet pepper variety with disease resistance and high quality. Journal of China Capsicum, 12(1):10-12. (in Chinese)
	薛庆华, 杨凤梅, 杨高强, 薛玉梅. 2014. 抗病、优质三系甜椒新品种沈研20号的选育. 辣椒杂志, 12(1):10-12.
[99]	Yamagishi H, Bhat S R. 2014. Cytoplasmic male sterility in Brassicaceae crops. Breeding Science, 64(1):38-47.
[100]	Yamagishi H. 1998. Distribution and allelism of restorer genes for Ogura cytoplasmic male sterility in wild and cultivated radishes. Genes and Genetic Systems, 73(2):79-83.
[101]	Yang Fengmei, Xue Qinghua, Yang Gaoqiang, Song Zhaohua. 2008. Breeding of Shenyan 13-a new three-lined hybrid pepper variety. Journal of China Capsicum,(2):7-8. (in Chinese)
	杨凤梅, 薛庆华, 杨高强, 宋兆华. 2008. 辣椒三系杂交种沈研13号选育. 辣椒杂志,(2):7-8.
[102]	Yang Juan, Wang Wen, Shen Huolin. 2010. SSR marker mapping and marker-assisted selection of Rf gene in pepper . China Cucurbits and Vegetables, 23(5):1-5. (in Chinese)
	杨娟, 王雯, 沈火林. 2010. 辣椒恢复基因SSR标记定位及分子标记辅助选择育种. 中国瓜菜, 23(5):1-5.
[103]	Yang Shizhou, Zhao Xueyun. 1984. Selection of male sterile line 8021A and utilization in three-lines breeding of pepper. China Vegetables,(3):9-13. (in Chinese)
	杨世周, 赵雪云. 1984. 辣椒8021A雄性不育系的选育及三系配套. 中国蔬菜,(3):9-13.
[104]	Yang Y Y, Huo Y M, Miao J, Liu B J, Kong S P, Gao L M, Liu C, Wang Z B, Tahara Y, Kitano H, Wu X. 2013. Identifcation of two SCAR markers co-segregated with the dominant Ms and recessive ms alleles in onion(Allium cepa L.). Euphytica, 190:267-277.
[105]	Yarrow S A, Burnett L A, Wildeman R P, Kemble R J. 1990. The transfer of‘Polima’cytoplasmic male sterility from oilseed rape(Brassica napus) to broccoli(B. oleracea)by protoplast fusion. Plant Cell Reports, 9(4):185-188.
[106]	Yasumoto K, Terachi T, Yamagishi H. 2009. A novel Rfgene controlling fertility restoration of Ogura male sterility by RNA processing of orf138 found in Japanese wild radish and its STS markers. Genome, 52(6):495-504.
[107]	Yu H L, Fang Z Y, Liu Y M, Yang L M, Zhuang M, Lv H H, Li Z S, Han F Q, Liu X P, Zhang Y Y. 2016. Development of a novel allele-specific Rfo marker and creation of Ogura CMS fertility-restored interspecific hybrids in Brassica oleracea. Theor Appl Genet, 129:1625-1637.
[108]	Yu H L, Li Z Y, Ren W J, Han F Q, Yang L M, Zhuang M, Lv H H, Liu Y M, Fang Z Y, Zhang YY. 2020. Creation of fertility-restored materials for Ogura CMS in Brassica oleracea by introducing Rfo gene from Brassica napus via an allotriploid strategy. Theor Appl Genet, 133(10):2825-2837.
[109]	Zhang B X, Huang S W, Yang G M, Guo J Z. 2000. Two RAPD markers linked to a major fertility restorer gene in pepper. Euphytica, 113(2):155-161.
[110]	Zhang Baoxi, Guo Jiazhen, Yang Guimei, Huang Sanwen, Du Meizhen. 2001. Breeding of sweet pepper CMS lines. Journal of China Capsicum,(1):10-12. (in Chinese)
	张宝玺, 郭家珍, 杨桂梅, 黄三文, 堵玫珍. 2001. 甜椒胞质雄性不育系的选育. 中国辣椒,(1):10-12.
[111]	Zhang Huamin. 2017. Map-based cloning od fertility restorer gene BrRfp1 for Pol CMS and mechanism research of different fertility restoration loci in Chinese cabbage(Brassica rapa L.)[Ph. D. Dissertation]. Yangling:Northwest A & F University. (in Chinese)
	张华敏. 2017. 大白菜Pol CMS恢复基因BrRfp1的图位克隆和不同恢复基因位点恢复机理的研究[博士论文]. 杨凌:西北农林科技大学.
[112]	Zhang Li, Wang Qiaobiao, Wang Yanping. 2020a. Molecular marker assisted selective breeding of radish male sterile line. China Vegetables,(2):40-44. (in Chinese)
	张丽, 王庆彪, 王艳萍. 2020a. 分子标记辅助选育萝卜雄性不育系. 中国蔬菜,(2):40-44.
[113]	Zhang Li, Wang Qingbiao, Wang Yanping. 2020b. Distribution of orf687,a fertility restorer gene for Ogura CMS in radish . Acta Horticulturae Sinica, 47(5):864-874. (in Chinese)
	张丽, 王庆彪, 王艳萍. 2020b. Ogura CMS育性恢复基因orf687在萝卜中的分布. 园艺学报, 47(5):864-874.
[114]	Zhang Li, Wang Qingbiao. 2020. A high throughput molecular marker for detection of fertility restorer gene in radish Ogura-CMS and its application. China:CN106967803B,2020-09-29. (in Chinese)
	张丽, 王庆彪. 2020. 一种检测萝卜Ogura-CMS育性恢复基因的高通量分子标记及应用. 中国:CN106967803B,2020-09-29.
[115]	Zhang Qiang, Yao Qiuju, Zhang Tao, Chang Xiaoke, Han Yanan, Cheng Zhifang, Liu Wei, Wang Bin, Ren Fusen, Guo Zhiwei, Sun Qiang, Chen Haofang, Liu Hejuan, Jiao Yushun. 2019. A KASP molecular marker,kit and application for identification of CMS male sterility restorer gene in pepper. China:CN109913575A,2019-06-21. (in Chinese)
	张强, 姚秋菊, 张涛, 常晓轲, 韩娅楠, 程志芳, 刘卫, 王彬, 任福森, 郭志伟, 孙强, 陈昊放, 刘贺娟, 焦禹顺. 2019. 一种鉴定辣椒CMS雄性不育恢复基因的KASP分子标记、试剂盒及其应用. 中国:CN109913575A,2019-06-21.
[116]	Zhang Xiaoli, Liu Yumei, Fang Zhiyuan, Yang Limei, Zhuang Mu, Zhang Yangyong, Li Zhansheng, Lü Honghao. 2016. Identification of germplasm resistant to clubroot(Plasmodiophora brassicae Woronin)in broccoli(Brassica oleracea L. var. italicaPlenck)and its relatives . Journal of Plant Genetic Resources, 17(6):1106-1115.
	张小丽, 刘玉梅, 方智远, 杨丽梅, 庄木, 张扬勇, 李占省, 吕红豪. 2016. 青花菜及近缘种属种质资源抗根肿病鉴定. 植物遗传资源学报, 17(6):1106-1115.
[117]	Zhang Yangyong, Yu Hailong, Fang Zhiyuan, Yang Limei, Zhuang Mu, Liu Yumei, Lü Honghao, Li Zhansheng. 2015. PCR marker for screening fertility restorer gene Rfo in Brassica oleraceaOgura cytoplasmic male sterility lines . China:CN104805212A,2015-07-29. (in Chinese)
	张扬勇, 于海龙, 方智远, 杨丽梅, 庄木, 刘玉梅, 吕红豪, 李占省. 2015. 用于甘蓝Ogura胞质不育系育性恢复基因Rfo筛选的PCR标记. 中国:CN104805212A,2015-07-29.
[118]	Zhang Z, Zhu Y, Cao Y, Yu H, Bai R, Zhao H, Zhang B, Wang L. 2020. Fine mapping of the male fertility restoration gene CaRf032 in Capsicum annuum L. Theor Appl Genet, 133(4):1177-1187.
[119]	Zhang Zhenghai, Wang Lihao, Cao Yacong, Yu Hailong, Zhang Baoxi, Zhu Yanshu. 2019. SNP markers related to recovery of cytoplasmic male sterility in pepper and their specific primers and application. China:CN110468225A,2019-11-19. (in Chinese)
	张正海, 王立浩, 曹亚从, 于海龙, 张宝玺, 朱砚姝. 2019. 辣椒细胞质雄性不育恢复性状相关的SNP标记及其特异性引物和应用. 中国:CN110468225A,2019-11-19.
[120]	Zhao Hualun, Ding Liping, Sun Jiebo, Wang Shubin, Qian Zhilong, Liu Jinbing, Yuan Caiyao. 1995. Breeding and identification of male sterile lines 21A,8A and 17A of hot(sweet)pepper. Jiangsu Agricultural Science,(1):49-50. (in Chinese)
	赵华仑, 丁犁平, 孙洁波, 王述彬, 钱芝龙, 刘金兵, 袁彩尧. 1995. 辣(甜)椒雄性不育系21A、8A、17A的选育及鉴定. 江苏农业科学,(1):49-50.
[121]	Zhou Yongjian. 2007. The location analysis of fertility restoration major gene in cytoplasmic male sterile pepper[M. D. Dissertation]. Changsha:Hunan Agricultural University. (in Chinese)
	周永坚. 2007. 辣椒胞质雄性不育恢复性主效基因位点分析[硕士论文]. 长沙:湖南农业大学.
[122]	Zou Xuexiao, Ma Yanqing, Dai Xiongze, Li Xuefeng, Chen Wenchao, Zhang Zhuqing. 2008. Seed production technique of cytoplasmic male sterile hybrid in Capsicum . China Vegetables, (5):45-47,73. (in Chinese)
	邹学校, 马艳青, 戴雄泽, 李雪峰, 陈文超, 张竹青. 2008. 辣椒胞质型雄性不育杂交种规模制种技术. 中国蔬菜,(5):45-47,73.
[123]	Zou Xuexiao, Zhou Qunchu, Dai Xiongze, Li Xuefeng, Ma Yanqing, Zhang Zhuqing, Liu Rongyun, Chen Wenchao. 2000. Breeding of pepper male Sterile line 9704A. Hunan Agricultural Sciences,(5):39-40. (in Chinese)
	邹学校, 周群初, 戴雄泽, 李雪峰, 马艳青, 张竹青, 刘荣云, 陈文超. 2000. 辣椒雄性不育系9704A的选育. 湖南农业科学,(5):39-40.
[124]	Zou Xuexiao, Zhou Qunchu, Dai Xiongze, Li Xuefeng, Ma Yanqing, Liu Rongyun, Chen Wenchao. 2001. Breeding of the new three-line hybrid hot pepper variety Xiangyan 14. Journal of China Capsicum,(1):13-16. (in Chinese)
	邹学校, 周群初, 戴雄泽, 马艳青, 李雪峰, 张竹青, 刘荣云, 陈文超. 2001. 辣椒三系雄性不育组合湘研14号的选育. 中国辣椒,(1):13-16.

选育单位 Breeding institution	细胞质不育系 CMS lines	恢复系 Restorer lines	用于配制的杂交品种 Hybrids produced by three-line system	参考文献 Reference
沈阳市农业科学院 Shenyang Academy of Agricultural Sciences	8021A,9911A,2013A,A02-7,A022	001-4-1,二斧头017-4-1,梅花椒099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5 001-4-1,Erfutou 017-4-1,Meihuajiao 099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5	沈研13,沈研14,沈研15,沈研20 Shenyan 13,Shenyan 14,Shenyan 15,Shenyan 20	杨世周和赵雪云,1984;杨凤梅等,2008;薛庆华等,2014
江苏省农业科学院蔬菜研究所 Institute of Vegetables,Jiangsu Academy of Agricultural Sciences	21A,8A,17A	LS₃,LS₂,LS₇,5-2R₁,5-2R₂,5-2R₃	苏椒3号A,碧玉,江蔬5号 Sujiao 3A,Biyu,Jiangshu 5	赵华仑等,1995;刘金兵等,2002,2004;王述彬等,2007
中国农业大学 China Agricultural University	8907A, S200243A	8965,8534,8318,S200244C	农大082 Nongda 082	沈火林等,1994
湖南省蔬菜研究所 Hunan Vegetable Research Institute	9704A,8214A,5901A,T01-55A,9202A	5904,8001,9701,	湘研14号,湘辣1号,湘辣2号 Xiangyan 14, Xiangla 1,Xiangla 2	邹学校等,2000,2008
广州蔬菜研究中心 Guangzhou Vegetable Research Center	21A,33A	52号,580号,17号	辣优2号,辣优8号,辣优9号 Layou 2,Layou 8,Layou 9	黄贞等,2006
中国农业科学院蔬菜花卉研究所 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences	77013A	Perennial		张宝玺等,2001; Wang et al.,2004
北京市农林科学院蔬菜研究中心 Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences	181A	98199	京辣2号 Jingla 2	耿三省等,2005

选育单位 Breeding institution	细胞质不育系 CMS lines	恢复系 Restorer lines	用于配制的杂交品种 Hybrids produced by three-line system	参考文献 Reference
沈阳市农业科学院 Shenyang Academy of Agricultural Sciences	8021A,9911A,2013A,A02-7,A022	001-4-1,二斧头017-4-1,梅花椒099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5 001-4-1,Erfutou 017-4-1,Meihuajiao 099-2,S4-1-7,S8-1-1-2,0840-7,03-6-3-5	沈研13,沈研14,沈研15,沈研20 Shenyan 13,Shenyan 14,Shenyan 15,Shenyan 20	杨世周和赵雪云,1984;杨凤梅等,2008;薛庆华等,2014
江苏省农业科学院蔬菜研究所 Institute of Vegetables,Jiangsu Academy of Agricultural Sciences	21A,8A,17A	LS₃,LS₂,LS₇,5-2R₁,5-2R₂,5-2R₃	苏椒3号A,碧玉,江蔬5号 Sujiao 3A,Biyu,Jiangshu 5	赵华仑等,1995;刘金兵等,2002,2004;王述彬等,2007
中国农业大学 China Agricultural University	8907A, S200243A	8965,8534,8318,S200244C	农大082 Nongda 082	沈火林等,1994
湖南省蔬菜研究所 Hunan Vegetable Research Institute	9704A,8214A,5901A,T01-55A,9202A	5904,8001,9701,	湘研14号,湘辣1号,湘辣2号 Xiangyan 14, Xiangla 1,Xiangla 2	邹学校等,2000,2008
广州蔬菜研究中心 Guangzhou Vegetable Research Center	21A,33A	52号,580号,17号	辣优2号,辣优8号,辣优9号 Layou 2,Layou 8,Layou 9	黄贞等,2006
中国农业科学院蔬菜花卉研究所 Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences	77013A	Perennial		张宝玺等,2001; Wang et al.,2004
北京市农林科学院蔬菜研究中心 Beijing Vegetable Research Center,Beijing Academy of Agricultural and Forestry Sciences	181A	98199	京辣2号 Jingla 2	耿三省等,2005

CMS类型 CMS type	恢复基因 Rf locus	标记类型(标记名称) Marker type(Marker name)	参考文献 Reference
Ogura CMS	Rfo,Rfk,Rft, Rfob,Rfoc, RsRf3-1/RsRf3-2,RsRf3-4 ~ RsRf3-7	RAPD(OPH11₄₁₀)、AFLP(AFLP₁₉₀),同工酶标记 Isozyme markers(pgi-2),AFLP(R3、R15、R5),RAPD(OPC02₁₁₅₀、OPD02₁₀₀₀、OPF06₁₂₀₀、OPG02₇₀₀),RFLP-PCR(2F/1R),KASP(Rfo-SNP1),RAPD(OPA-14₆₀₀), SCAR(SCC06-1894),STS(STS-A14、STS-K17)	Delourme & Eber,1992;Delourme et al.,1994;Murayama et al.,1999,2002;Brown et al.,2003;Desloire et al.,2003;Wang et al.,2008,2013,2017;Yasumoto et al.,2009;张丽和王庆彪,2020
DCGMS	Rfd1	RAPD-PCR(R624、A137、A220)	Kim et al.,2010;Cho et al.,2012

CMS类型 CMS type	恢复基因 Rf locus	标记类型(标记名称) Marker type(Marker name)	参考文献 Reference
Ogura CMS	Rfo,Rfk,Rft, Rfob,Rfoc, RsRf3-1/RsRf3-2,RsRf3-4 ~ RsRf3-7	RAPD(OPH11₄₁₀)、AFLP(AFLP₁₉₀),同工酶标记 Isozyme markers(pgi-2),AFLP(R3、R15、R5),RAPD(OPC02₁₁₅₀、OPD02₁₀₀₀、OPF06₁₂₀₀、OPG02₇₀₀),RFLP-PCR(2F/1R),KASP(Rfo-SNP1),RAPD(OPA-14₆₀₀), SCAR(SCC06-1894),STS(STS-A14、STS-K17)	Delourme & Eber,1992;Delourme et al.,1994;Murayama et al.,1999,2002;Brown et al.,2003;Desloire et al.,2003;Wang et al.,2008,2013,2017;Yasumoto et al.,2009;张丽和王庆彪,2020
DCGMS	Rfd1	RAPD-PCR(R624、A137、A220)	Kim et al.,2010;Cho et al.,2012

标记名称 Marker name	标记类型 Marker type	遗传距离/cM Genetic distance	参考文献 Reference
OP13₁₄₀₀、OW19₈₀₀	rapd	0.37、8.12	Zhang et al.,2000
OPL09_-763	rapd	4.18	唐冬英等,2004
AFRF8-CAPS	aflp-caps	1.8	Kim et al.,2006
CRF3S1S	scar	4.8 ~ 5.3	Gulyas et al.,2006
E32M49-424bp、q18-1.0kb	aflp、rapd	3.1、2.2	周永坚,2007
PR-CAPS	caps	1.8	Lee et al.,2008a
CaRf-M1	rapd-pcr	1.1	Min et al.,2008
CRFCAPS、AFRF4/CaRf-FL-M	caps、aflp/sts	1.5、0.1/0.5	Min et al.,2009
BAC13T7SCAR、BAC17T7SCAR、PepPR1AS-PCR	scar	1.4、3.2、14	Jo et al.,2010
AF208834	ssr	20.8	杨娟等,2010
Co1Mod1-CAPS	caps	0	Jo et al.,2016
Capsicum-R-82CAPS	caps	0	林多等,2020
CaRf648	caps	0	Ortega et al.,2020

Advances on Fertility Restoration for Cytoplasmic Male Sterility in Vegetables

RichHTML

PDF

Knowledge

Abstract

Cite this article

share this article

Figures/Tables 3

References 124

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CUI Jian, ZHONG Xionghui, LIU Zeci, CHEN Denghui, LI Hailong, HAN Rui, YUE Xiangqing, KANG Jungen, WANG Chao. Construction of Cabbage Chromosome Segment Substitution Lines [J]. Acta Horticulturae Sinica, 2023, 50(1): 65-78.
[2]	LIU Yiping, NI Menghui, WU Fangfang, LIU Hongli, HE Dan, KONG Dezheng. Association Analysis of Organ Traits with SSR Markers in Lotus（Nelumbo nucifera） [J]. Acta Horticulturae Sinica, 2023, 50(1): 103-115.
[3]	New Spring Cabbage Cultivars‘Zhonggan ’and‘Zhonggan ’. New Spring Cabbage Cultivars‘Zhonggan 27’and‘Zhonggan 28’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 63-64.
[4]	ZHOU Na, TAO Weilin, LU Jingwei, ZHENG Yang, HU Yan, and PAN Xiaoxue. A New Cabbage Cultivar‘Yuyuan Chunyin’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 65-66.
[5]	WANG Huizhe, YA NG Ruihuan, DENG Qiang, CAO Mingming, and LI Shuju, . A New Cucumber Cultivar‘Jindong 369’Resistant to Scab [J]. Acta Horticulturae Sinica, 2022, 49(S1): 85-86.
[6]	WANG Sha, ZHANG Xinhui, ZHAO Yujie, LI Bianbian, ZHAO Xueqing, SHEN Yu, DONG Jianmei, YUAN Zhaohe. Cloning and Functional Analysis of PgMYB111 Related to Anthocyanin Synthesis in Pomegranate [J]. Acta Horticulturae Sinica, 2022, 49(9): 1883-1894.
[7]	YANG Xingyu, XU Linbing, WU Yuanli, QIU Diyang, FAN Linlin, HUANG Bingzhi. Genome（ABBB）Identification of a Hybrid Banana Cultivar‘Fenza 1’ [J]. Acta Horticulturae Sinica, 2022, 49(9): 1991-1997.
[8]	QIU Ziwen, LIU Linmin, LIN Yongsheng, LIN Xiaojie, LI Yongyu, WU Shaohua, YANG Chao. Cloning and Functional Analysis of the MbEGS Gene from Melaleuca bracteata [J]. Acta Horticulturae Sinica, 2022, 49(8): 1747-1760.
[9]	ZHENG Lin, WANG Shuai, LIU Yunuo, DU Meixia, PENG Aihong, HE Yongrui, CHEN Shanchun, ZOU Xiuping. Gene Cloning and Expression Analysis of NAC Gene in Citrus in Response to Huanglongbing [J]. Acta Horticulturae Sinica, 2022, 49(7): 1441-1457.
[10]	ZHANG Lugang, LU Qianqian, HE Qiong, XUE Yihua, MA Xiaomin, MA Shuai, NIE Shanshan, YANG Wenjing. Creation of Novel Germplasm of Purple-orange Heading Chinese Cabbage [J]. Acta Horticulturae Sinica, 2022, 49(7): 1582-1588.
[11]	LIU Yaoyao, WU Yanyan, Shi Yan, MAO Tianyu, BAO Manzhu, ZHANG Junwei, ZHANG Jie. Preliminary Study on the Relationship Between Promoter Sequence Difference of PmTAC1 and Weeping Trait of Prunus mume [J]. Acta Horticulturae Sinica, 2022, 49(6): 1327-1338.
[12]	LI Shasha, YU Saisai, FU Yuheng, LUO Qiangwei, XU Yan, WANG Yuejin. The Embryo Rescue and Molecular Markers are Used to Breed New Seedless,Cold-Resistant Grapes [J]. Acta Horticulturae Sinica, 2022, 49(4): 723-738.
[13]	QIAO Jun, LIU Jing, LI Suwen, WANG Liying. Prediction of Fruit Color Genes Under the Calyx of Eggplant Based on Genome-wide Resequencing in an Extreme Mixing Pool [J]. Acta Horticulturae Sinica, 2022, 49(3): 613-621.
[14]	SHENG Yunyan, YANG Limin, DAI Dongyang, ZHANG Jiaxin, WANG Ling, WANG Di, CAI Yi, TIAN Limei. Cytological Observation of Fruit Peduncle Abscission Zone and Preliminary Mapping of Mature Fruit Abscission AL3 gene in Melon [J]. Acta Horticulturae Sinica, 2022, 49(2): 341-351.
[15]	SONG Mengfei, ZHA Gaohui, CHEN Jinfeng, LOU Qunfeng. Research Progress on Molecular Basis of Plant Architecture Related Traits in Cucumber [J]. Acta Horticulturae Sinica, 2022, 49(12): 2683-2702.