Identification,Growth Conditions and Fungicide Sensitivity of the Pathogen Causing Black Spot Disease on Peony Leaves

doi:10.16420/j.issn.0513-353x.2020-0236

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 173-182.doi: 10.16420/j.issn.0513-353x.2020-0236

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Identification,Growth Conditions and Fungicide Sensitivity of the Pathogen Causing Black Spot Disease on Peony Leaves

TAO Hang, ZAYINA Mahabat, ZHANG Ye, SUN Litong, HUANG Shenxin, ZHANG Zihui, LIU Wang, SHI Ningxue, CHEN Xiaoren^**()

College of Horticulture and Plant Protection,Yangzhou University,Yangzhou,Jiangsu 225009,China

Received:2020-09-22 Revised:2020-12-04 Online:2021-01-25 Published:2021-01-29
Contact: CHEN Xiaoren E-mail:xrchen@yzu.edu.cn

Abstract

Abstract:

A black spot disease was discovered on peony（Paeonia lactiflora）leaves in Yangzhou City,Jiangsu Province in 2017. To determine the causing agent,the fungus was isolated and purified from the diseased leaves,and was tested for its pathogenicity following Koch’s postulation. The fungus was identified as Alternaria alternata based on its morphological characteristics and the combined sequence analyses of rDNA-ITS,endoPG,OPA2-1 and Alt a 1. Analysis of its growth conditions showed that the optimal growth conditions of A. alternata on Potato Dextrose Agar（PDA）include the temperature at 28 ℃ and pH 7.0. On defined Czapek media,soluble starch and KNO₃ are the optimal carbon source and nitrogen source,respectively. What’s more,among four fungicides tested in toxicity assays,both Azoxystrobin and Pyraclostrobin exhibited more stronger inhibition effect on the mycelial growth of the pathogen on PDA,compared with Tebuconazole and Iprodione.

Key words: peony, leaf black spot disease, Alternaria, growth conditions, fungicide

CLC Number:

S682.1

TAO Hang, ZAYINA Mahabat, ZHANG Ye, SUN Litong, HUANG Shenxin, ZHANG Zihui, LIU Wang, SHI Ningxue, CHEN Xiaoren. Identification,Growth Conditions and Fungicide Sensitivity of the Pathogen Causing Black Spot Disease on Peony Leaves[J]. Acta Horticulturae Sinica, 2021, 48(1): 173-182.

Figures/Tables 8

References 23

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种名 Species	菌株 Isolate	NCBI基因数据库登录号 GenBank accession
种名 Species	菌株 Isolate	rDNA-ITS	endoPG	OPA2-1	Alt a 1
链格孢菌Alternaria alternata	EGS 34-016	AF347031	AY295024	AY295065	KP275691
	EGS 34-039	―	AY295025	AY295064	―
	39-190	―	AY629218	AY295060	―
	S2	MT269274	MT505726	MT505728	MT505725
橘树链格孢Alternaria citriarbusti	SH-MIL-15s	―	AY295026	AY295057	―
橘斑链格孢Alternaria citrimacularis	BC2-RLR-32s	JX397903	JX397906	AY295069	―
草莓链格孢Alternaria fragaria	0-187	―	AY629222	AY631462	―
梨黑斑链格孢Alternaria gaisen	EGS 90-0512	―	AY295033	AY631460	KP275694
粗柠檬链格孢Alternaria limoniasperae	PR325	―	AY629233	AY295062	―
蜜柚链格孢Alternaria tangelonis	EV-MIL-2s	―	AY295031	AY295066	JQ646392
细极链格孢Alternaria tenuissima	EGS 34-015	AF347032	JQ811977	AY631463	―
土耳其链格孢Alternaria turkisafria	EGS 44-159	―	AY295022	AY295054	JQ646404

种名 Species	菌株 Isolate	NCBI基因数据库登录号 GenBank accession
种名 Species	菌株 Isolate	rDNA-ITS	endoPG	OPA2-1	Alt a 1
链格孢菌Alternaria alternata	EGS 34-016	AF347031	AY295024	AY295065	KP275691
	EGS 34-039	―	AY295025	AY295064	―
	39-190	―	AY629218	AY295060	―
	S2	MT269274	MT505726	MT505728	MT505725
橘树链格孢Alternaria citriarbusti	SH-MIL-15s	―	AY295026	AY295057	―
橘斑链格孢Alternaria citrimacularis	BC2-RLR-32s	JX397903	JX397906	AY295069	―
草莓链格孢Alternaria fragaria	0-187	―	AY629222	AY631462	―
梨黑斑链格孢Alternaria gaisen	EGS 90-0512	―	AY295033	AY631460	KP275694
粗柠檬链格孢Alternaria limoniasperae	PR325	―	AY629233	AY295062	―
蜜柚链格孢Alternaria tangelonis	EV-MIL-2s	―	AY295031	AY295066	JQ646392
细极链格孢Alternaria tenuissima	EGS 34-015	AF347032	JQ811977	AY631463	―
土耳其链格孢Alternaria turkisafria	EGS 44-159	―	AY295022	AY295054	JQ646404

碳源 Carbon source	菌落直径/mm Colony diameter	氮源 Nitrogen source	菌落直径/mm Colony diameter
蔗糖Sucrose（对照Control）	45.1 ± 3.9 cde	硝酸钾KNO₃（对照Control）	44.8 ± 0.8 a
葡萄糖Dextrose	43.4 ± 2.5 e	硝酸铵NH₄NO₃	34.5 ± 1.1 b
麦芽糖 Maltose	63.8 ± 4.1 a	硝酸钙Ca(NO₃)₂	42.0 ± 2.0 a
乳糖Lactose	59.7 ± 1.8 ab	氯化铵NH₄Cl	35.2 ± 2.7 ab
可溶性淀粉Soluble starch	67.1 ± 5.3 a	尿素Urea	26.2 ± 1.4 c
木糖Xylose	55.6 ± 1.7 abc	谷氨酸Glutamic acid	27.9 ± 2.2 c
半乳糖Galactose	49.4 ± 1.9 bcd

碳源 Carbon source	菌落直径/mm Colony diameter	氮源 Nitrogen source	菌落直径/mm Colony diameter
蔗糖Sucrose（对照Control）	45.1 ± 3.9 cde	硝酸钾KNO₃（对照Control）	44.8 ± 0.8 a
葡萄糖Dextrose	43.4 ± 2.5 e	硝酸铵NH₄NO₃	34.5 ± 1.1 b
麦芽糖 Maltose	63.8 ± 4.1 a	硝酸钙Ca(NO₃)₂	42.0 ± 2.0 a
乳糖Lactose	59.7 ± 1.8 ab	氯化铵NH₄Cl	35.2 ± 2.7 ab
可溶性淀粉Soluble starch	67.1 ± 5.3 a	尿素Urea	26.2 ± 1.4 c
木糖Xylose	55.6 ± 1.7 abc	谷氨酸Glutamic acid	27.9 ± 2.2 c
半乳糖Galactose	49.4 ± 1.9 bcd

药剂名称 Fungicide name	浓度/（mg · L^-1） Concentration					毒力回归方程 Toxicity regression equation	R²	EC₅₀/（mg · L^-1）
药剂名称 Fungicide name	1	0.5	0.1	0.05	0.01	毒力回归方程 Toxicity regression equation	R²	EC₅₀/（mg · L^-1）
嘧菌酯Azoxystrobin	39.6 ab	34.0 b	30.9 ab	20.2 b	6.2 b	y = 0.1595x + 0.4086	0.9301	3.7415 b
吡唑醚菌酯 Pyraclostrobin	45.1 a	40.7 a	33.4 a	31.8 a	20.6 a	y = 0.1165x + 0.4503	0.9843	2.6706 a
戊唑醇Tebuconazole	24.0 c	17.8 c	17.4 c	14.4 c	10.4 bc	y = 0.0592x + 0.2224	0.8870	4.8876 × 10⁴ c
异菌脲Iprodione	5.5 d	5.2 d	4.9 d	4.2 d	2.4 d	y = 0.0147x + 0.0577	0.8798	1.2260 × 10³⁰ d

Identification,Growth Conditions and Fungicide Sensitivity of the Pathogen Causing Black Spot Disease on Peony Leaves

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