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ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (7): 1383-1390.doi: 10.16420/j.issn.0513-353x.2019-0760

• Research Notes • Previous Articles     Next Articles

Distribution,Molecular Characterization and Genome Sequence of Actinidia virus A(AcVA)and Actinidia virus B(AcVB)in Shaanxi

ZHAO Lei1,ZHANG Yuanle1,HUANG Qianru1,HOU Caiting1,WU Kuan1,3,CAO Mengji4,WANG Qiaochun1,2,*,and WU Yunfeng1,*   

  1. 1State Key Laboratory of Crop Stress Biology for Arid Areas,College of Plant Protection,Yangling,Shaanxi 712100,China;2College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China;3Yangling Vocational & Technical College,Yangling,Shaanxi 712100,China;4Citrus Research Institute,Southwest University,Chongqing 400712,China
  • Online:2020-07-25 Published:2020-07-25

Abstract: Kiwifruit(Actinidia spp.)viruses has seriously damaged kiwifruit production. This study investigated the incidence and distribution of Actinidia virus A(AcVA)and Actinidia virus B(AcVB)in four major kiwifruit cultivars(‘Xuxiang’,‘Hayward’,‘Huayou’and‘Qinmei’)grown in Shaanxi. The results showed that AcVA and AcVB were widely spread,with high infection rates in Shaanxi. Among the four main kiwifruit producing areas tested in this study,the highest infection rates for AcVA and AcVB were obtained in Yangling(36.7%)and Meixian(36.9%). Among the four tested kiwifruit cultivars,the highest infection rates for AcVA(32.3%)and AcVB(32.9%)were obtained in‘Xuxiang’. This study conducted next-generation sequencing and obtained the genome sequence of AcVA-ZZ and AcVB-ZZ isolates which consisted of 7 654 and 7 454 nucleotides,respectively. The genomes of AcVA-ZZ and AcVB-ZZ isolates were 82.7% and 78.5% homologous with those of TP7-93A and TP7-93B isolates previously reported. Phylogenetic trees of AcVA and AcVB coat protein gene showed substantial sequence variations and therefore were considered as two distinct clades.

Key words: Actinidia, Actinidia virus A, Actinidia virus B, genome;molecular variability, virus detection

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