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ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (6): 1087-1097.doi: 10.16420/j.issn.0513-353x.2019-0738

• Research Papers • Previous Articles     Next Articles

Cloning and Functional Analysis of PhTs2 Gene in Petunia hybrida

TIAN Shaoze1,LIU Siyu1,2,WANG Kun1,YUE Yuanzheng1,3,MA Fangfang1,YANG Zhaonan1,YIN Qian1,and HU Huirong1,*   

  1. 1College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Key Laboratory of Urban Agriculture in Central China,Ministry of Agriculture and Rural Affairs,Key Laboratory of Horticultural Plant Biology,Ministry of Education,Wuhan 430070,China;2Wuhan Qintai Greening Engineering Co.,Ltd.,Wuhan 430070,China;3College of Landscape Architecture,Nanjing Forestry University,Nanjing 210037,China
  • Online:2020-06-25 Published:2020-06-25

Abstract: PhTs2,a gene related to flower development,was cloned from Petunia hybrida‘Fantasy Red’. The CDS of this gene is 855 bp long and encodes 284 amino acids with a conserved adh-short domain. The phylogenetic analysis showed that the PhTs2 protein was closely related to the homologous proteins in tobacco and tomato. Quantitative real-time PCR analysis indicated that the gene was specifically expressed in the early developmental stage of anther in petunia. To analyze its function,an overexpression vector of PhTs2 was constructed and transformed into tobacco,then the transgenic tobaccos were identified:Phenotypic observation of transgenic tobaccos showed abnormal anther morphology and reduced pollen count;pollen vigor and germination rate were extremely reduced;the malformed pollen grains with irregular outer wall texture was observed by scanning electron microscopy;the semi-thin section showed the disorder of the tapetum development and the abnormal morphology of pollen grains. This study provides potential genetic resources for the cultivation of male sterile lines.

Key words: Petunia hybrida, fertility;anther, tapetum, male sterility, pollen

CLC Number: