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Acta Horticulturae Sinica ›› 2020, Vol. 47 ›› Issue (12): 2451-2458.doi: 10.16420/j.issn.0513-353x.2020-0769

• New Technologies and New Methods • Previous Articles     Next Articles

Efficient Identification of Tetraploid Plants from Seedling Populations of Apomictic Citrus Genotypes Based on Morphological Characteristics

ZHOU Rui1,XIE Kaidong1,WANG Wei1,PENG Jun1,XIE Shanpeng1,HU Yibo2,WU Xiaomeng1,and GUO Wenwu1,*   

  1. 1Key laboratory of Horticultural Plant Biology(Ministry of Education),College of Horticulture and Forestry Sciences,Huazhong Agricultural University,Wuhan 430070,China;2Citrus Research Institute of Deqing County,Zhaoqing,Guangdong 526600,China
  • Online:2020-12-25 Published:2021-01-06

Abstract: Based on the phenomenon of spontaneous doubling of nucellar cells in polyembryonic citrus genotypes,we established a simple and efficient approach for citrus tetraploid exploitation by sowing the seeds(after germination being accelerated in a 28 ℃ incubator for about one week)in pots in a controlled growth chamber,followed by screening tetraploids from germinated seedlings based on root,leaf morphology,oil gland density and flow cytometry analysis. By this method,we screened 5,6,5,17,45 and 7 putative tetraploids respectively from the 432,506,1 356,1 870,2 749 and 685 seedlings of Succari sweet orange,Hongjiang sweet orange,Gonggan mandarin,Nian mandarin,Xinhui mandarin and Huapi kumquat. By analyzing their ploidy level using flow cytometry,a total of 72 tetraploids was obtained,including 4 from Succari sweet orange,5 from Gonggan mandarin,5 from Hongjiang sweet orange,14 from Nian mandarin,39 from Xinhui mandarin and 5 from Huapi kumquat,with the tetraploid pre-screening accuracy rate of 80.0%,83.3%,100.0%,82.4%,86.7% and 71.4%,respectively. The merits of our new method are higher accuracy rate of tetraploid pre-screening,and time saving only needing less than 40 d from seed sowing to tetraploid confirmation. Simple sequence repeat(SSR)analysis showed that the bands of all tetraploids obtained herein are identical with that of their corresponding diploid genotypes,indicating that all these plants are the doubled diploids which have great potential for seedless triploid breeding.

Key words: citrus, polyploid, flow cytometry, SSR molecular marker

CLC Number: