https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2020, Vol. 47 ›› Issue (12): 2397-2404.doi: 10.16420/j.issn.0513-353x.2020-0068

• Research Notes • Previous Articles     Next Articles

The Disease Development of Apple Scar Skin Viroid in the Field and Its Transmission Mode in Tissue Culture

XI Nana,ZHAO Ke,YANG Jinfeng,MENG Xianglong,HU Tongle,WANG Shutong,WANG Yanan*,and CAO Keqiang*   

  1. College of Plant Protection,Agricultural University of Hebei,Baoding,Hebei 071001,China
  • Online:2020-12-25 Published:2021-01-06

Abstract: Apple scar skin disease caused by apple scar skin viroid(ASSVd)is common in apple producing areas northern in China and it has led to serious economic losses in recent years. At present,there are no effective treatment measures. In order to clarify the disease development and transmission mode in the field,three orchards in Nanshennan Village,Shunping,Baoding were continuously investigated and tested from 2012 to 2015. In orchard A,B,C,the change of symptom rate from 3.00%,19.35% and 10.00% to 10.00%,34.41% and 22.00%,and the change of virus-taking rate from 11.00%,20.43% and 14.00% to 23.00%,37.63% and 30.00%. The rate of symptom-showing and virus-taking increased year by year,and the virus-taking rate was greater than the symptom-showing rate. The incidence of fruit trees in the field distribution has an obvious incidence center. Some fruit tree taking virus did not show symptom. By high-throughput sequencing method,it was found that the number of ASSVd vsiRNA reads in symptomatic fruit trees was 4.08 times that of asymptomatic fruit trees with virus,and the accumulation of ASSVd in symptomatic fruit trees was significantly higher than that in asymptomatic fruit trees with virus by quantitative real-time PCR detection. ASSVd-carrying tissue culture seedlings and non-ASSVd- carrying tissue culture seedlings were used as test materials to simulate different transmission modes in the field,and RT-PCR was used to confirm that ASSVd could be transmitted through wound contamination,tissue culture shear pollution,virus-taking medium and root contact,among which the root contact transmission rate was the highest.

Key words: apple, apple scar skin viroid, high-throughput, RT-PCR, disease development, transmission mode

CLC Number: