Abstract: Effects of graphene oxide（GO）on the growth and physiological characteristics of turfgrass were studied by pot experiment. The Festuca arundinacea was planted in soil with three levels（1%，2% and 3%）of GO added. The results showed that the contents of chlorophylls and carotenoids in Festuca arundinacea all significantly decreased under GO treatments，and the accumulation of dry weight was suppressed. The biomass and the contents of photosynthetic pigments decreased with the increase of GO treatment level. Compared with the control，the biomass，content of total chlorophylls and carotenoids were decreased by 14.0%，25.8% and 36.2%，respectively in first harvest，and by 19.2%，25.6% and 62.3%，respectively in the second harvest under 3% GO treatment. Increases of malondialdehyde（MDA）content and peroxidase（POD）activity were observed with the increment of GO treatment，while activities of catalase （CAT）and superoxide dismutase（SOD）increased first and then decreased with the increase of GO level. The CAT and SOD activities reached the highest values in 2% and 1% GO treatment levels，respectively. Therefore，the addition of GO had induced the oxidative stress in F. arundinacea，inhibited plant growth，and the inhibition was more significant at the high treatment level.

Key words: Festuca arundinacea, graphene oxide, physiological characteristic, oxidative stress