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ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (1): 1-10.doi: 10.16420/j.issn.0513-353x.2019-0171

• Research Papers •     Next Articles

Analysis of Abscisic Acid Sensitivity of Apple Ethylene Response Factor MdERF11 Gene

WANG Jiahui,YU Jianqiang,ZHANG Quanyan,HAN Pengliang,YOU Chunxiang,HU Dagang*,and HAO Yujin   

  1. State Key Laboratory of Crop Biology,Ministry of Agriculture Key Laboratory of Horticultural Crop Biology(Huanghuai Region)and Germplasm Innovation,College of Horticulture Science and Engineering,Shandong Agricultural University,Tai’an,Shandong 271018,China
  • Online:2020-01-25 Published:2020-01-25

Abstract: An ethylene response factor(GenBank accession number:MDP0000756341),named as MdERF11,was isolated from‘Gala’apple cultivar(Malus × domestica Borkh.),with a complete open reading frame(ORF)of 483 bp in length and encodes 161 amino acids. Phylogenetic tree analysis showed that the ethylene response factor exhibited the highest sequence similarity with Arabidopsis AtERF11. In silico analysis suggested that the promoter sequence of MdERF11 contained several typical cis-acting elements,including abscisic acid-,ethylene- and drought-responsive elements by using PlantCare Databases. qRT-PCR assays showed that MdERF11 constitutively expressed in different tissues of apple,with higher expression levels in petioles and fruits. The expression of MdERF11 was noticeably induced by exogenous abscisic acid(ABA). Furthermore,the growth potential of MdERF11-overexpressing apple calli were much stronger than that of wild-type apple calli under exogenous ABA condition,suggesting that MdERF11 reduces the sensitivity of apple callus to exogenous ABA.

Key words: apple, MdERF11, ethylene response factor, abscisic acid

CLC Number: