Abstract: In the present study，CmCRL1（MELO3C012908）was cloned by PCR approach from‘L8’melon strain（Cucumis melo L.）. The tissue-specific transcription pattern of CmCRL1 and responses to low temperature，sorbitol，PEG6000，salt and waterlogging stresses were analyzed by real-time PCR method. CmCRL1 gene contained two exons and one intron. The length of cDNA sequence was 996 bp and the open reading frame（ORF）was 732 bp which encoded a protein of 243 amino acids. Sequence analysis showed that CmCRL1 habored a highly conserved LOB domain，belonging to the LBD/AS2（Lateral Organ Boundaries Domain/Asymmetric Leaves2）family. Multiple alignment analysis revealed that the LOB domain of CmCRL1 had a high conservation with Csa3G396920，Cla005992，AtLBD16，AtLBD29，OsCRL1，ZmRTCS and ZmRTCL，with sequence consistency of 100%，99.02%，74.51%，86.27%，88.24%，89.22% and 80.39%，respectively. Transcriptional expression pattern showed that CmCRL1 was predominantly expressed in roots. The expression level of CmCRL1 in roots was 178.39，7.54，26.95，65.19 and 75.86 times higher than in cotyledons，hypocotyls，epicotyls，leaves and shoot tips. Furthermore，CmCRL1 was identified as being responsive to low temperature，sorbitol，PEG6000 and salt stresses. In addition，CmCRL1 was strongly up-regulated during adventitious root development under waterlogging stress in melon.

Key words: melon, CmCRL1, gene cloning, gene expression