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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (5): 1023-1030.doi: 10.16420/j.issn.0513-353x.2020-1004

• Research Notes • Previous Articles     Next Articles

Molecular Characteristic and Expression Level of Minor Coat Protein of Citrus Tristeza Virus Isolates with Different Transmissibility by Toxoptera citricida

LIU Jinxiang1, WANG Huanhuan1, WANG Hongsu1,2, ZHOU Yan1, LI Zhongan1, ZHOU Changyong1,*()   

  1. 1Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China
    2Sichuan Academy of Chinese Medicine Sciences,Chengdu 610041,China
  • Received:2021-01-26 Revised:2021-03-05 Online:2021-05-25 Published:2021-06-07
  • Contact: ZHOU Changyong E-mail:zhoucy@cric.cn

Abstract:

The minor coat protein(CPm)of citrus tristeza virus(CTV)is necessary in virus transmission by the brown citrus aphid[Toxoptera citricida(Kirkaldy)]. The expression levels of CPm in three isolates with high,middle and low aphid transmissibility were conducted by Western blot technique. Then,the CPm sequences of CTLJ and CT16-2 isolates were acquired by RT-PCR,cloning and sequencing. Finally,these amino acid sequences were used to analyze the multiple alignments by the DNAStar program. The result showed that the identity of amino acid sequence of CPm for CTLJ with low aphid transmissibility was only 93.3%-94.2%,when compared with those of other CTV isolates. Comparison of the CPm of these isolates showed that nine of fourteen amino acid mutation site were unique in the CTLJ isolate. Particularly,some mutations led to substitution of new amino acids with a different charge and polarity,which resulted in changes of the secondary structure of the CPm protein in CTLJ. However,the expression of CPm for CTLJ,CT16-2 and CT11A did not show a significant difference. The results suggest that the aphid transmission efficiency of CTV might be not related to the expression level of CPm,but might be associated with amino acid site variation.

Key words: citrus tristeza virus(CTV), minor coat protein(CPm), expression level, molecular characteristics

CLC Number: