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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (9): 1743-1754.doi: 10.16420/j.issn.0513-353x.2020-0710

• Research Papers • Previous Articles     Next Articles

Genome-wide Identification and Expression Analysis Under Temperature Stress of HSP70 Gene Family in Dendrobium catenatum

ZHAO Xi1, ZHANG Tingting1, XING Wenting3, WANG Jian2, SONG Xiqiang2, ZHOU Yang1,**()   

  1. 1Key Laboratory for Quality Regulation of Tropical Horticultural Plants of Hainan Province / Key Laboratory of Germplasm Resources Biology of Tropical Special Ornamental Plants of Hainan Province / Hainan Key Laboratory for Biotechnology of Salt Tolerant Crops,School of Horticulture,Hainan University,Haikou 570228,China
    2Key Laboratory of Ministry of Education for Genetics and Germplasm Innovation of Tropical Special Trees and Ornamental Plants,School of Forestry,Hainan University,Haikou 570228,China
    3Hainan Academy of Forestry,Haikou 571000,China
  • Received:2021-03-04 Revised:2021-05-07 Online:2021-09-25 Published:2021-09-30
  • Contact: ZHOU Yang E-mail:zhouyang@hainanu.edu.cn

Abstract:

In order to study the resistant mechanism of Dendrobium catenatum response to temperature stress,the heat shock protein 70(HSP70)gene families were identified using bioinformatics methods. Meanwhile,the expression profiles of HSP70 genes in different tissues and under high(42 ℃)and low(4 ℃)temperatures were assayed by quantitative real time PCR(qRT-PCR)method. The results showed that 10 HSP70 genes were identified from D. catenatum,which encode 628 to 702 amino acids, the molecular weight was between 69.65 to 75.15 kD,and the theoretical isoelectric point ranged from 5.12 to 6.18. The HSP70 proteins were divided into four subgroups by phylogenetic tree analysis,and the conserved motifs of each subgroup were similar. Structural analysis showed that the number of introns in HSP70 gene varied from one to seven. Spatial expression pattern analysis showed that HSP70 gene expression was tissue specific and the highest expression was found in gynostemium. The expression levels of DcHSP7-1 and DcHSP70-7 increased after low temperature treatment,which indicated that DcHSP70-1 and DcHSP70-7 responded to cold stress. While the relative expression of DcHSP70-2,DcHSP70-3,DcHSP70-5 and DcHSP70-7 was up-regulated obviously under high temperatuer,then we can speculate that these genes play key roles in response to heat stress.

Key words: Dendrobium catenatum, HSP70 gene family, characteristic analysis, temperature stress, expression analysis

CLC Number: