https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1565-1578.doi: 10.16420/j.issn.0513-353x.2020-0660

• Research Papers • Previous Articles     Next Articles

Expression Pattern and Regulation Mechanism of ApSK3 Dehydrin (Agapanthus praecox)Response to Abiotic Stress and Hormone Signals

YANG Tianchen, CHEN Xiaotong, LÜ Ke, ZHANG Di()   

  1. School of Design,Shanghai Jiao Tong University,Shanghai 200240,China
  • Received:2021-02-09 Revised:2021-06-03 Online:2021-08-25 Published:2021-09-06
  • Contact: ZHANG Di E-mail:zhangdi2013@sjtu.edu.cn

Abstract:

This study cloned the dehydrin ApSK3Agapanthus praecox)2 195 bp promoter sequence of ApSK3-P using real-time PCR technology to analyze tissue specificity of ApSK3 gene,and gene expression pattern response to various abiotic stress and ABA signal. Expression vectors with five promoter deletion fragments of ApSK3-P were constructed and transformed into Arabidopsis thaliana,in order to reveal the response pattern of ApSK3 gene to different stress and hormone signals and the regulatory function of cis-acting elements. Bio-informatic analysis showed that the ApSK3-P contained many typical cis-acting elements related to plant stress,hormone response and plant development. ApSK3 gene was tissue-specific expression,the highest expression was found in fruits,followed by leaves and roots,and the lowest value was shown in flowers. ApSK3 is more sensitive to ABA and salt signals,followed by drought and low temperature,and no obvious response to high temperature. ApSK3-P::GUS were transformed into A. thaliana and it showed that ApSK3-P presented strong activity in seedlings of A. thaliana,GUS activity of roots was stronger than that of leaves,and the activity gradually increased in the developing fruits. Different promoter deletion fragments of ApSK3-P test results showed that-2 175 to-950 bp fragment played an important role in the regulation of promoter activity,multiple cis-acting elements responsed to drought stress,two tandem ABRE elements participated in response to ABA signals,and ApSK3-P can be activated by ERE(-526 to-533 bp)and P-box(-561 to-567 bp)under ethylene and gibberellin signal,respectively. These results indicated that ApSK3-P can be activated by drought,cold,salt,ABA,GA and ethylene,and ABRE,ERE and P-box elements of ApSK3-P play important roles in response to abiotic stress and hormone signals.

Key words: Agapanthus praecox, dehydrin, promoter, abiotic stress, cis-acting element

CLC Number: