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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (8): 1446-1456.doi: 10.16420/j.issn.0513-353x.2020-0630

• Research Papers • Previous Articles     Next Articles

Functional Analysis of Sucrose-phosphate Synthase Genes(SPS)in Sweet Cherry

QI Xiliang, LIU Congli, SONG Lulu, LI Ming()   

  1. Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China
  • Received:2020-12-10 Revised:2021-04-26 Online:2021-08-25 Published:2021-09-06
  • Contact: LI Ming E-mail:liming06@caas.cn

Abstract:

In order to ascertain the function of sucrose-phosphate synthase genes(SPS)in the regulation of sweet cherry ripening,four full-length cDNA of sweet cherry SPS genes(PavSPSA1,PavSPSA2,PavSPSB,and PavSPSC)were cloned in sweet cherry genome. Gene expression analysis showed that the expression levels of PavSPSA1 was the highest,followed by PavSPSC,whereas PavSPSA2 and PavSPSB were relatively weakly expressed during the fruit growth and development,and ripening using quantitative real-time PCR. The expression levels of PavSPSA1 and PavSPSA2 coincided with fruit ripening and softening. Furthermore,we used TRV-mediated virus-induced gene silencing(VIGS)to evaluate the function of PavSPSA1,PavSPSA2,PavSPSB,and PavSPSC in the regulation of ripening. The results showed that silencing PavSPSA1 gene decreased sucrose content,delayed fruit coloring,ripening and softening. However,The fruit phenotypes for the PavSPSA2,PavSPSB,and PavSPSC-silenced fruits showed no visible defects when compared with the control fruits. Taken together,our findings determined that PavSPSA1 may be a key gene regulating sucrose synthesis and accumulation in sweet cherry fruit and play an indispensable role in the regulation of sweet cherry fruit ripening and softening.

Key words: sweet cherry, sucrose-phosphate synthase, PavSPSA1, gene expression, fruit ripening, VIGS

CLC Number: