Analysis of Resistance Mechanism to Powdery Mildew Based on Transcriptome Sequencing in Malus hupehensis

doi:10.16420/j.issn.0513-353x.2020-0578

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (5): 860-872.doi: 10.16420/j.issn.0513-353x.2020-0578

• Research Papers • Previous Articles Next Articles

Analysis of Resistance Mechanism to Powdery Mildew Based on Transcriptome Sequencing in Malus hupehensis

LAN Liming¹, LUO Changguo²^,^*(), WANG Sanhong¹^,^*()

¹Laboratory of Fruit Tree Biology,College of Horticulture,Nanjing Agricultural University,Nanjing 210018,China
²Institute of Fruits Sciences,Guizhou Academy of Agricultural Sciences,Guiyang 550006,China

Received:2020-09-08 Revised:2021-03-05 Online:2021-05-25 Published:2021-06-07
Contact: LUO Changguo,WANG Sanhong E-mail:376258195@qq.com;wsh3xg@niau.edu.cn

Abstract

Abstract:

To explore the pathways and mechanism of response to powdery mildew in Malus hupehensis(Pamp.)Rehder,we used the BGISEQ-500 sequencing technology and de novo transcriptome assembly to gain a comprehensive overview of the transcriptome of the leaves of‘Qingzhen 1’, ‘Qingzhen 1’/Malus hupehensis and M. hupehensis after the outbreak of powdery mildew in the same period. The resistance to powdery mildew of three materials increased in turn. Gene Ontology(GO)enrichment showed significant enrichment of GO term associated with metabolic process and catalytic activity in Qingzhen 1,Qingzhen 1/Malus hupehensis and M. hupehensis. KEGG analysis showed linoleic acid metabolism,plant-pathogen interaction,inositol phosphate metabolism,brassinosteroid biosynthesis and phosphatidylinositol signaling system etc. were significantly enriched in Qingzhen 1/Malus hupehensis and resistance to powdery mildew,respectively. And the carbon metabolism pathway was only significantly enriched in M. hupehensis. Expression analysis of genes related to the resistance to powdery mildew showed that WRKY70,WRKY33,NPR3and WRKY40 were significantly up-regulated in Qingzhen 1/Malus hupehensis and M. hupehensis compared to Qingzhen 1. The accuracy of sequencing were confirmed by qRT-PCR experiment.

Key words: apple, Malus hupehensis, rootstock, rootstock-scion interaction, powdery mildew, transcriptome, differentially expressed

CLC Number:

S661.4

LAN Liming, LUO Changguo, WANG Sanhong. Analysis of Resistance Mechanism to Powdery Mildew Based on Transcriptome Sequencing in Malus hupehensis[J]. Acta Horticulturae Sinica, 2021, 48(5): 860-872.

Figures/Tables 7

References 49

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样品名称 Sample name	clean reads数量/Mb Number of clean reads	GC含量/% GC content	Q30/%	低质量含量/% Low quality content
青砧1号Qingzhen 1	46.17(96.40%)	49.40	93.31	3.09
青砧1号/湖北海棠 Qingzhen 1/Malus hupehensis	56.53(97.25%)	47.33	93.98	2.34
湖北海棠 M. hupehensis	52.78(96.64%)	48.53	93.50	2.87

样品名称 Sample name	clean reads数量/Mb Number of clean reads	GC含量/% GC content	Q30/%	低质量含量/% Low quality content
青砧1号Qingzhen 1	46.17(96.40%)	49.40	93.31	3.09
青砧1号/湖北海棠 Qingzhen 1/Malus hupehensis	56.53(97.25%)	47.33	93.98	2.34
湖北海棠 M. hupehensis	52.78(96.64%)	48.53	93.50	2.87

基因名称 Gene name	基因ID Gene ID	Nr-注释 Nr-annotation	引物序列(5'-3') Primer sequence(5'-3')
WRKY70	XP_008388364.1^a	WRKY转录因子70[苹果] WRKY transcription factor 70[Malus × domestica]	F:AAACCACCTACATCGGCGAG
WRKY70	XP_008388364.1^a		R:ATGAGAATTGACGGTGCGGA
WRKY33	NP_001315684.1^a	WRKY转录因子26[苹果] WRKY transcription factor 26[Malus × domestica]	F:ACAACAACAGCGCCTCTACC
WRKY33	NP_001315684.1^a		R:CCCTCCCCAAAAGACAGCAAA
WRKY3	XP_008352971.1^a	WRKY转录因子3[苹果] WRKY transcription factor 3[Malus × domestica]	F:TACACAGGCAACAACAACGC
WRKY3	XP_008352971.1^a		R:TCTGATTCCTCGTGCTTGCTT
WRKY22	XP_008345081.1^a	WRKY转录因子22[苹果] WRKY transcription factor 22[Malus × domestica]	F:CTAGTAGTGCTGCCACTGCC
WRKY22	XP_008345081.1^a		R:TCCCACCCATAGTTACACCCA
WRKY2	XP_008344758.1^a	WRKY转录因子2[苹果] WRKY transcription factor 2[Malus × domestica]	F:TCTTGGTGCAACGCCTTGAT
WRKY2	XP_008344758.1^a		R:TCTCGAGCTCCCAGGAAGAA
NPR3	ANJ45443.1^a	NPR5a [苹果] NPR5a[Malus × domestica]	F:CATCTTCTCCGAAGGACGGG
NPR3	ANJ45443.1^a	NPR5a [苹果] NPR5a[Malus × domestica]	R:TCTGTTTGATAAGGAGAGAGCCA
WRKY40	NP_001315769.1^a	WRKY转录因子40[苹果] WRKY transcription factor 40[Malus × domestica]	F:TGCTCAACTCCAAGGCAATG
WRKY40	NP_001315769.1^a		R:TTCACTGTCTGCCTTTCGCT
EF1	MD09G014760^b	内参基因 Reference gene	F:TACTGGAACATCACAGGCTGAC
EF1	MD09G014760^b	内参基因 Reference gene	R:TGGACCTCTCAATCATGTTGTC

基因名称 Gene name	基因ID Gene ID	Nr-注释 Nr-annotation	引物序列(5'-3') Primer sequence(5'-3')
WRKY70	XP_008388364.1^a	WRKY转录因子70[苹果] WRKY transcription factor 70[Malus × domestica]	F:AAACCACCTACATCGGCGAG
WRKY70	XP_008388364.1^a		R:ATGAGAATTGACGGTGCGGA
WRKY33	NP_001315684.1^a	WRKY转录因子26[苹果] WRKY transcription factor 26[Malus × domestica]	F:ACAACAACAGCGCCTCTACC
WRKY33	NP_001315684.1^a		R:CCCTCCCCAAAAGACAGCAAA
WRKY3	XP_008352971.1^a	WRKY转录因子3[苹果] WRKY transcription factor 3[Malus × domestica]	F:TACACAGGCAACAACAACGC
WRKY3	XP_008352971.1^a		R:TCTGATTCCTCGTGCTTGCTT
WRKY22	XP_008345081.1^a	WRKY转录因子22[苹果] WRKY transcription factor 22[Malus × domestica]	F:CTAGTAGTGCTGCCACTGCC
WRKY22	XP_008345081.1^a		R:TCCCACCCATAGTTACACCCA
WRKY2	XP_008344758.1^a	WRKY转录因子2[苹果] WRKY transcription factor 2[Malus × domestica]	F:TCTTGGTGCAACGCCTTGAT
WRKY2	XP_008344758.1^a		R:TCTCGAGCTCCCAGGAAGAA
NPR3	ANJ45443.1^a	NPR5a [苹果] NPR5a[Malus × domestica]	F:CATCTTCTCCGAAGGACGGG
NPR3	ANJ45443.1^a	NPR5a [苹果] NPR5a[Malus × domestica]	R:TCTGTTTGATAAGGAGAGAGCCA
WRKY40	NP_001315769.1^a	WRKY转录因子40[苹果] WRKY transcription factor 40[Malus × domestica]	F:TGCTCAACTCCAAGGCAATG
WRKY40	NP_001315769.1^a		R:TTCACTGTCTGCCTTTCGCT
EF1	MD09G014760^b	内参基因 Reference gene	F:TACTGGAACATCACAGGCTGAC
EF1	MD09G014760^b	内参基因 Reference gene	R:TGGACCTCTCAATCATGTTGTC

Analysis of Resistance Mechanism to Powdery Mildew Based on Transcriptome Sequencing in Malus hupehensis

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[10]	WANG Qiang, CONG Peihua, and LIU Xiaofeng. A New Mid-ripening Apple Cultivar‘Huayou Baomi’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 5-6.
[11]	YANG Ling, CONG Peihua, WANG Qiang, LI Wuxing, and KANG Liqun. A New Mid-ripening Apple Cultivar‘Huafeng’ [J]. Acta Horticulturae Sinica, 2022, 49(S1): 7-8.
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