https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 49-59.doi: 10.16420/j.issn.0513-353x.2020-0215

• Research Papers • Previous Articles     Next Articles

Nectarine Stem Pitting-associated Virus and Asian Prunus Virus Found in Prunus persica of Xinjiang

BU Fangdi1, CHEN Junguang1, LIU Zhen2, XIANG Benchun2, SHEN Mian1, CUI Baiming1,*(), ZHENG Yinying1,*()   

  1. 1College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003,China
    2College of Agriculture,Shihezi University,Shihezi,Xinjiang 832003,China
  • Received:2020-06-02 Revised:2020-09-07 Online:2021-01-25 Published:2021-01-29
  • Contact: CUI Baiming,ZHENG Yinying E-mail:2247237543@qq.com;69825983@qq.com

Abstract:

In this study,the High-throughput sequencing technology(HTS)was used to detect the virus infection of Prunus persica var. compressa. The peach leaves showing shot hole and chlorosis between leaf veins were collected to extract total RNA for HTS. A total of 13.36 Gb sequence data,including 44 563 187 paired reads,were obtained. The sequence alignment analysis showed that there were 9 943 and 40 036 paired reads belong to the genome of nectarine stem pitting-associated virus(NSPaV)(KT273409)and asian prunus virus(APV2)(KT893294). The contigs of 4 978 nt and 9 393 nt with nearly completed coverage of viruses genome(10 and 7 bases missing at 5′ end,respectively)were assembled and the sequence identity with the genome sequence of NSPaV(KT273409)and APV2(KT893294)were 95.0% and 92.9% respectively. We named them as isolates NSPaV-Tao and APV2-Tao4.To confirm the result of HTS,23 samples of P. persica var. compressa were detected by the RT-PCR method for the infection of NSPaV and APV2. The results showed that the infection rate of NSPaV and APV2 was 43.5% and 69.6%,respectively. Moreover,NSPaV-Tao may be a recombination of other NSPaV isolates based on the nucleotide sequence comparison.

Key words: NSPaV, APV2, Prunus persica var. compressa, high-throughput sequencing, RT-PCR, sequence analysis

CLC Number: