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Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 127-136.doi: 10.16420/j.issn.0513-353x.2020-0208

• Research Notes • Previous Articles     Next Articles

Cloning of MhMYB114-Like from Malus halliana and Its Functional Identification of Iron Deficiency Tolerance in Apple Callus

ZHANG Zhongxing, CHENG Li, WANG Shuangcheng, ZHANG De, LIU Bing, WANG Yanxiu*()   

  1. College of Horticulture,Gansu Agricultural University,Lanzhou 730070,China
  • Received:2020-05-06 Revised:2020-07-10 Online:2021-01-25 Published:2021-01-29
  • Contact: WANG Yanxiu E-mail:wangxy@gsau.edu.cn

Abstract:

The MhMYB114-Like gene(GenBank accession number:LOC103405832)was cloned from apple rootstock Malus halliana. The gene contains a full open reading frame of 602 bp,which encodes 200 amino acids,and the protein has an isoelectric point of 9.38. The phylogenetic tree analysis showed that apple MhMYB114-Like was the closest relative to wild strawberry. Promoter cis-element prediction of apple MhMYB114-Like showed that there were drought,light,auxin,yellowing and other responsive regulatory elements. Fluorescence quantitative PCR analysis demonstrated that the expression level of MhMYB114-Like gene was greatly increased in the leaves and roots of Malus halliana under iron deficiency condition,and the expression was obviously induced by iron deficiency stress. In addition,The genetic transformation of MhMYB114-Like into apple‘Orin’callus and resistance analysis by measuring related physiological indexes showed that the overexpression of MhMYB114-Like could significantly improve the resistance of transgenic callus to iron deficiency stress. Therefore,MhMYB114-Like is involved in the response to iron deficiency and play a crucial role in resisting iron deficiency stress.

Key words: apple, MhMYB114-Like, functional identification, iron deficiency stress, gene expression

CLC Number: