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Acta Horticulturae Sinica ›› 2020, Vol. 47 ›› Issue (12): 2405-2414.doi: 10.16420/j.issn.0513-353x.2020-0170

• Research Notes • Previous Articles     Next Articles

Cloning and Expression Analysis of CsNCED3-2 in Responding to Citrus Canker Disease

XIE Yu,ZHANG Qingwen,QI Jingjing,ZOU Xiuping,HE Yongrui,XU Lanzhen,LEI Tiangang,PENG Aihong,LI Qiang,YAO Lixiao,CHEN Shanchun*,and LONG Qin*   

  1. Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,National Citrus Engineering Research Center,Chongqing 400712,China
  • Online:2020-12-25 Published:2021-01-06

Abstract: Citrus canker is an important disease of citrus in the world and causes severe economic losses to the citrus industry. In this study,the 9-cis-epoxycarotenoid dioxygenase gene(NCED)family of Citrus sinensis were annotated,and the citrus canker related CsNCED3-2 was cloned and its expression pattern was analyzed. A total of 14 NCED family members were annotated from the public genomic databases of C. sinensis. These 14 NCED family members can be divided into 4 subclasses based on the phylogenetic tree and functional domain. The full-length of CsNCED3-2 is 2 377 bp with a 1 830 bp open reading frame which codes a protein containing 609 amino acids. The CsNCED3-2 gene belongs to the members of the RPE65 superfamily and the carotenoid dioxygenase family,and it is a non-secretory protein. After inoculated with Xanthomonas citri subsp. citri(Xcc),the expression of CsNCED3-2 in Jindan(Fortunella crassifolia Swingle)was always higher than that in Wanjincheng orange(Citrus sinensis Osbeck)at different time points,and the variation trends of expression in the two varieties were opposite. The expression of CsNCED3-2 in the peel of Wanjincheng Orange and Jindan were higher than that in the stem,leaf and seed. The CsNCED3-2 promoters of Wanjincheng Orange and Jindan contain multiple cis-acting elements involved in plant adversity and hormone responses,such as ABRE(abscisic acid response),TCA-element(salicylic acid response),MYC/MYB(drought response)and so on,but there are differences in their number and type.

Key words: citrus, canker disease, NCED, gene cloning, expression level, promoter

CLC Number: