Correlation Analysis of Citrus Catalase Gene CsKat01 and Citrus Canker Disease

doi:10.16420/j.issn.0513-353x.2020-0132

Acta Horticulturae Sinica ›› 2021, Vol. 48 ›› Issue (1): 26-36.doi: 10.16420/j.issn.0513-353x.2020-0132

• Research Papers • Previous Articles Next Articles

Correlation Analysis of Citrus Catalase Gene CsKat01 and Citrus Canker Disease

QI Jingjing, QIN Xiujuan, XIE Yu, CHEN Shanchun, HE Yongrui^*(), LI Qiang^*()

Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,Chongqing 400712,China

Received:2020-06-05 Revised:2020-09-06 Online:2021-01-25 Published:2021-01-29
Contact: HE Yongrui,LI Qiang E-mail:heyongrui@cric.cn;liqiang@cric.cn

Abstract

Abstract:

In order to investigate the correlation between CsKat01 and citrus bacterial canker disease （CBCD）response,bioinformatics and expression analysis of CsKat01 as well as the relationship between CAT activity and Hydrogen peroxide（H₂O₂）contents in CBCD susceptible variety Wanjincheng and resistant variety Calamondin were performed. Firstly,the coding sequence（CDS）of the CsKat01 gene was cloned and the structure and function of the coding protein were analyzed. The full-length CDS of CsKat01 is 1 482 bp and it encodes 493 amino acid residues with a typical catalase domain. Secondly,by cloning and analyzing the promoter sequence,we studied the promoter regulatory elements and found that the core promoters of both Wanjincheng and Calamondin contained salicylic acid（SA）,jasmonic acid（JA）and abscisic acid（ABA）response elements whose numbers are different. Thirdly,qRT-PCR was used to analyze the induced expression profiles of plant hormones and Xanthomonas citri subsp. citri（Xcc）on CsKat01,and then the correlation between the enzyme and CBCD was determined. Finally,the relations between CsKat01,CAT activity,H₂O₂ content and CBCD resistance were analyzed. We speculate that CsKat01 may regulate CAT enzyme activity,which further regulates H₂O₂ accumulation,and then regulates CBCD resistance.

Key words: citrus, bacterial canker disease, catalase, CsKat01, hydrogen peroxide

CLC Number:

S666

QI Jingjing, QIN Xiujuan, XIE Yu, CHEN Shanchun, HE Yongrui, LI Qiang. Correlation Analysis of Citrus Catalase Gene CsKat01 and Citrus Canker Disease[J]. Acta Horticulturae Sinica, 2021, 48(1): 26-36.

Figures/Tables 7

Fig. 1 Bioinformatics analysis of CsKat01

Fig. 2 Multiple alignment of catalases of several organisms Cs:Citrus sinensis;Egl:Eucalyptus globulus;Vv:Vitis vinifera;Nt:Nicotiana tabacum;Pt:Populus trichocarpa;At:Arabidopsis thaliana.

Fig. 3 The phylogenetic tree of CAT from different species The number on the branch represents the reliability of the branch,and the length represents the evolutionary distance.

Table 1 cis-Acting regulatory elements in promoter of CsKat01 predicted by Plant CARE

元件名称 Cis elements	功能 Funtion	序列 Sequence	位置 Position		方向 Strand
元件名称 Cis elements	功能 Funtion	序列 Sequence	晚锦橙 Wanjincheng	四季橘 Calamondin	晚锦橙 Wanjincheng	四季橘 Calamondin
ABRE	脱落酸响应 ABA-responsiveness	ACGTG	733	733	+	+
				1 536		-
CCAAT-box	MYBHv1结合位点 MYBHv1 binding site	CAACGG	1 307	1 320	-	-
CGTCA-motif	茉莉酸响应 JA-responsiveness	CGTCA	943	1 470	+	-
			1 455		-
TCA-element	水杨酸响应元件 SA-responsiveness	CCATCTTTTT	1 298	1 128	-	+

Fig. 4 The relative expression of CsKat01 induced by SA,JA and ABA The data significant difference of Calamondin and Wanjincheng is indicated by a and a’ respectively,and different letters indicate significant difference,P < 0.05. The same below.

Fig. 5 The relative expression of CsKat01 induced by Xcc infection

Fig. 6 CAT enzyme activitie and H2O2 content induced by Xcc infection* P < 0.05;** P < 0.01.

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Correlation Analysis of Citrus Catalase Gene CsKat01 and Citrus Canker Disease

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