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ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (7): 1301-1311.doi: 10.16420/j.issn.0513-353x.2020-0119

• Research Papers • Previous Articles     Next Articles

Cloning and Expression Under Abiotic Stress of Br14-3-3 in Brassica rapa subsp. rapifera

WANG Ruonan,LI Ju,MIAO Hongyu,and YAN Haifang*   

  1. College of Life Sciences,Northeast Forestry University,Harbin 150040,China
  • Online:2020-07-25 Published:2020-07-25

Abstract: In this study,we cloned the general regulatory factor 14-3-3 gene from Brassica rapa subsp. rapifera‘Tsuda’,which was named Br14-3-3 with the accession number of MK896872 in the NCBI GenBank. The full cDNA sequence of the Br14-3-3 gene was 1 113 bp,cotaining an open reading frame of 774 bp encoding a protein of 257 amino acids. We determined the expression of the Br14-3-3 gene in different tissues and under different abiotic stress conditions,such as extreme temperature,dehydration,adverse osmosis,abscisic acid(ABA)and salt stress by quantitative-PCR analysis. The results demonstrated that the highest expression levels of the Br14-3-3 gene could be reached in the flower of Brassica rapa subsp. rapifera‘Tsuda’,followed by the seedling. The expression of the Br14-3-3 gene was upregulated under aforementioned stress conditions except for low temperature,suggesting that the Br14-3-3 gene may play a role in response of abiotic stresses.

Key words: Brassica rapa, 14-3-3, gene cloning, abiotic stress, gene expression

CLC Number: