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ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (3): 432-444.doi: 10.16420/j.issn.0513-353x.2019-403

• Research Papers • Previous Articles     Next Articles

Interacting Protein Screening and Analysis of CsAP2-09 — A Citrus Bacterial Canker Related Transcription Factor

QI Jingjing*,DOU Wanfu*,ZHANG Qingwen,HU Anhua,CHEN Shanchun,LEI Tiangang,PENG Aihong,XU Lanzhen,YAO Lixiao,HE Yongrui**,and LI Qiang**   

  1. Citrus Research Institute,Southwest University/Chinese Academy of Agricultural Sciences,National Citrus Engineering Technology Research Center,Chongqing 400712,China
  • Online:2020-03-25 Published:2020-03-25

Abstract: CsAP2-09 is a positive transcription factor involved in the citrus bacterial canker resistance. In this study,the GST pull-down and LC–MS/MS strategies were used for the screening and network analysis of the interacting proteins of CsAP2-09. Firstly,the fusion proteins GST-CsAP2-09 were expressed and purified for the next GST pull-down. Then GST-CsAP2-09 was fixed on the GST-beads and incubated with total proteins extracted from CsAP2-09 over-expression plant. The interacting proteins of CsAP2-09 were detected by LC–MS/MS. From the annotation,GO and KEGG,17 interacting proteins were obtained,5 of which were reported to be involved in bacterial resistance,e.g. Cs3g27280(CAT)-a catalase.The interacting network was constructed,among which 14 of 17 interacting proteins were involved. Sixteen proteins were positively regulated by CsAP2-09 in the transgenic plant while 1 protein was negatively regulated.

Key words: citrus, citrus bacterial canker(CBC), GST pull-down, CsAP2-09, AP2/ERF, interacting protein

CLC Number: