Abstract: Receptor like kinase（RLK）take the crucial role in plant growth，development and environmental adaptation. In previous studies，one kind of apple（Malus × domestica）had glyco_18 RLK with extracellular domain（SM000636 or SM000704）named Glyco_18-RLK（MdGly-RLK）. In this test， Glyco_18 and Pkinase（SM000220）conserved domain whole protein sequences were seed sequences. Gly-RLK in 53 angiosperms was identified and its evolutionary characteristics were analyzed. Based on bioinformatics expression analysis，the physical and chemical properties，subcellular location，gene distribution on chromosome，cis-elements and expression patterns of promoter region of apple Gly-RLK were determined. The results showed that only 19 species of plants had Gly-RLK，and the number of family members ranged from 1 to 20. No family members were found in all monocotyledonous plants. According to the evolutionary analysis，it was divided into 8 subgroups，with more genes distributed in subgroup Ⅲ，Ⅶ and Ⅷ. We identified 6 Gly-RLKs from apple genome. Their amino acid sequence size，molecular weight and isoelectric point were 451–776，50.99–87.33 kD，and 5.95–8.21，respectively. They were mainly located in plasma membrane，and four of them were tandem repeat. There were different expression patterns of MdGly-RLK in different apple tissues and varieties. After inoculation with Valsa mali，the expression of six MdGly-RLKs in apples was different，and MdGly-RLK6（MD15G1156900）could be increased to 16.33 times of the control. To sum up，Gly-RLK only exist in some dicotyledons，and the expansion speed of family members is faster. MdGly-RLK can respond to the signals of apple growth and development and black rot fungus. MdGly-RLK6 can be used as a candidate gene for the study of apple anti rot.

Key words: apple, Glyco_18-RLK（Gly-RLK）, evolutionary characteristics, expressional patterns, Valsa canker