Abstract: The distribution of orf687（Rfo），a fertility restorer gene of Ogura CMS，was investigated in 129 radish cultivars，including 5 cultivated species and 4 wild species. Our results showed that orf687 was widely distributed in East Asian big radish（89.16%），European small radish（85.71%），black radish （100%），pod radish（100%）and wild radish（70%）. Among East Asian big radish，95.45% of the red-skinned radish contained orf687，which was higher than that of white radish and green radish. In addition，genotype identification of orf687 by PCR-RFLP showed that the ratio of double recessive genotype（rfrf）without restoring ability was the highest in wild radish（57.14%），followed by East Asia big radish（47.13%）and European small radish（37.50%）. Moreover，in East Asian big radish，the ratio of red-skinned varieties with rfrf genotype was 76.19%，higher than that of white radish（41.67%）and green radish（23.53%）. The results clarified the Rfo genotypes of different radish varieties，and will provide guidance for the breeding and utilization of male sterile lines.

Key words: radish, Ogrua CMS, Rfo gene, distribution