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ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (6): 1194-1202.doi: 10.16420/j.issn.0513-353x.2019-0716

• Review • Previous Articles     Next Articles

A Review of Reduced-representation Genome Sequencing Technique and Its Applications in Ornamental Plants

XIONG Yan1,2,ZHANG Jinzhu1,*,DONG Jie1,WANG Li1,GAO Peng1,JIANG Wan1,and CHE Daidi1,*   

  1. 1College of Horticulture,Northeast Agricultural University,Harbin 150030,China;2Institute of Natural Resources and Ecology,Heilongjiang Academy of Sciences,Harbin 150040,China
  • Online:2020-06-25 Published:2020-06-25

Abstract: Reduced-representation Genome Sequencing(RRGS)was based on the second-generation high-throughput sequencing technology in which the genome was interrupted by restriction endonuclease and a large number of genetic polymorphism tag sequences were obtained by high-throughput sequencing of specific regions of the genome,and then revealing the characteristics of the entire Genome sequence. This method has the advantages of simple process of library building,cost-effective,and can effectively reduce the complexity of genome. At present,RRGS has been widely used in the development of molecular markers,population genetics and phylogeny,construction of high-density genetic map and quantitative characteristics localization of ornamental plants. In this paper,the technical principle of RRGS and its research progress in ornamental plants were reviewed,the problems and development prospects were also discussed.

Key words: reduced-representation genome sequencing, ornamental plant, high-throughput sequencing, SNP

CLC Number: