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ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (7): 1320-1332.doi: 10.16420/j.issn.0513-353x.2018-0932

• Research Papers • Previous Articles     Next Articles

Pathogenicity Differentiation and ISSR Genetic Variation Analysis of Verticillium dahliae Isolates from Eggplant in Northeast China

BEN Haiyan1,3,QU Hongyun1,GAO Wei3,ZHANG Junmin1,ZHANG Xueyan1,LI Baoju2,*,and CHAI Ali2,*   

  1. 1Gardening Branch,Heilongjiang Academy of Agricultural Sciences,Harbin 150069,China;2Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China;3Institute of Plant Protection,Tianjin Academy of Agricultural Sciences,Tianjin 300381,China
  • Online:2019-07-25 Published:2019-07-25

Abstract: This study revealed the genetic variation of eggplant Verticilium dahlia in Northeast China by studying the characteristics,pathogenicity and ISSR(Inter-simple sequence repeat)genetic variation of 80 representative eggplant V. dahliae strains collected from 49 major eggplant-growing counties in four northeastern provinces. The results showed that 80 tested strains were divided into 4 different culture types (A to D)according to cultural characteristics on PDA medium. Among them,sclerotia type isolates were the most(56.25% of the tested strains)and mycelium type isolates were the least(7.5% of the tested strains). The pathogenicity test showed that 80 isolates had obvious pathogenicity differentiation and could be classified into three pathogenic types,i.e. strong(Ⅰ),moderate(Ⅱ)and weak(Ⅲ),which occupied for 26.25%,31.25% and 42.50% of the total tested strains,respectively. According to the results of PCR,the deciduous isolates which accounted for 31.25% were mainly moderate and strong pathogenic strains;the non-deciduous strains which accounted for 68.75% were mostly weak pathogenic strains. ISSR analysis indicated that the genetic variation of 80 isolates was small and related to geographic region.

Key words: eggplant, Verticillium dahliae, culturing characteristics, pathogenicity, pathogenicity type, ISSR

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