https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
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ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (8): 1429-1436.doi: 10.16420/j.issn.0513-353x.2016-0242

    Next Articles

Molecular Cloning and Functional Characterization of a Cytokinin O-glycosyltransferase Gene MdZOG1 in Apple

AN Jian-ping1,*,SONG Lai-qing2,*,ZHAO Ling-ling2,YOU Chun-xiang1,WANG Xiao-fei1,**,and HAO Yu-jin1,**   

  1. 1College of Horticulture Science and EngineeringShandong Agricultural UniversityState Key Laboratory of Crop BiologyTai’anShandong 271018China2Yantai Academy of Agricultural SciencesYantaiShandong 265599China
  • Online:2016-08-25 Published:2016-08-25

Abstract:

The cytokinin O-glycosyltransferase gene MdZOG1 was cloned by combination of the homologous clone and PCR technology from apple(Malus × domestica‘Gala’). The Open Reading Frame (ORF)of MdZOG1 contained 762 bp,encoding a protein of 253 amino acid residues. Phylogenetic analysis showed that MdZOG1 had the highest evolutionary relationship with PbZOG1. The transcriptional analysis results showed that expression of MdZOG1 was mainly in root and stem,the expression of MdZOG1 in flower and fruit tissues was lower than other tissues. Transgenic Arabidopsis and tobacco were generated by Agrobacterium-mediated genetic transformation. Ectopic expression MdZOG1 in Arabidopsis and tobacco significantly enhanced resistance to water stress,indicating that cytokinin O-glycosyltransferase played an important role in plant drought stress.

Key words: apple, cytokinin O-glycosyltransferase, MdZOG1, drought stress

CLC Number: