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园艺学报 ›› 2003, Vol. 30 ›› Issue (1): 113-114.

• 研究报告 • 上一篇    下一篇

袋鼠花的组织培养

刘春;穆鼎   

  1. (中国农业科学院蔬菜花卉研究所, 北京100081)
  • 收稿日期:2002-01-24 修回日期:2002-05-15 出版日期:2003-02-25 发布日期:2003-02-25

The Studies of the Tissue Culture of Anigozanthos ( Kangaroo paws)

Liu Chun;Mu Ding   

  1. (Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences, Bejing 100081 , China)
  • Received:2002-01-24 Revised:2002-05-15 Online:2003-02-25 Published:2003-02-25

摘要: 对袋鼠花( Anigozanthos) 组织培养研究表明: 外植体消毒以0. 1 %升汞溶液浸泡, 7~8 min 为宜; 不同浓度激素对其愈伤组织形成、分化以及根的形成有不同的影响, 随着62BA 浓度的增加, 愈伤组织的诱导率有增加的趋势; 生长素与分裂素的比例决定着愈伤组织的分化; 小苗在1/ 2 MS 培养基上生根最好, 6-BA 对其生根有抑制作用。各培养阶段最适培养基: (1) 愈伤, MS + 6-BA 1 mg/L + NAA 0. 1 mg/L +3 %活性炭; (2) 芽诱导, MS + 6-BA 0. 5 mg/L + NAA 0. 5 mg/L ; (3) 生根, 1/ 2 MS + IBA 0. 2 mg/L。

关键词: 袋鼠花, 组织培养

Abstract: The results of current study show that the optimum sterilization of explants is 7 -8 min with 0. 1 %HgCl2. The concentration of NAA , 6-BA affects the formation of callus , shoot induction and rooting of plantlets.A increase in 6-BA concentration resulted in increased callus formation. The BA/ NAA ratio determined the shoot induction. Half strength MS mediumproved best for the rooting plantlets and the addition of 6-BA inhibited it . The best media for different cultural stages are : (1) callus : MS + 6-BA 1 mg/ L + NAA 0. 1 mg/ L + charcoal 3 %;(2) shoot induction : MS + 6-BA 0. 5 mg/ L + NAA 0. 5 mg/ L ; (3) rooting : 1/ 2 MS + IBA 0. 2 mg/ L.

Key words: Anigozanthos (Kangaroo paws), Tissue culture

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