关键词: 高羊茅, 草坪草, 农杆菌, 转化, 真菌

Abstract: Lawn is playing a more and more important role in humanps life, but its fungi disease is a important problem which should be solved urgently in the maintenance of lawn. In the paper, the binary vector harboring Glu and Chi gene was transformed into calli of tall fescue mediated byAg robacterium tum efacien, and the suitable transformation protocol of Agrobacterium of tall fescue was established. In the transformation, we confirmed that, the optimum concentration of selective agent kanamycin was 200 - 300 mg/L, the combination of carbercillin and cefotaxine as inhibitive agents had good inhibitive effect to Agrobacterium; The results of several cocultivation methods of Agrobacterium showed that, putting some filter paper on cocultivation medium, and dripping infection solution of Ag robacterium around the calli, and usingmannitol and antibiotic solution to washing calli 1 week after resuming cultivation, was the best way of transformation. The results of molecular
detection of PCR and Southern-blotting and challenge inoculation experiment with F. graminearum Schwabe showed that, transformed p lants had higher positive frequency and resistance to fungi disease than the control respectively.

Key words: Tall fescue, Turfgrass, Agrobacterium tumefacien, Transformation, Fungi