五唇兰菌根化育苗技术

园艺学报 ›› 2008, Vol. 35 ›› Issue (4): 571-576.

五唇兰菌根化育苗技术

柯海丽¹ ;宋希强^1,2 ^*;罗毅波²;朱国鹏¹;凌绪柏³

（1 海南大学园艺园林学院，海南儋州 571737； 2 中国科学院植物研究所，系统与进化植物学国家重点实验室，北京 100093；3 海南博大兰花科技有限公司，海口 571101）

收稿日期:2007-12-17 修回日期:2008-02-22 出版日期:2008-04-25 发布日期:2008-04-25
通讯作者: 宋希强

Seedling Cultivation of Doritis pulcherrima Lindl. with Mycorrhizal Fungi

KE Hai-li ¹,SONG Xi-qiang ^1,2 ^*,LUO Yi-bo ², ZHU Guo-peng¹,and LING Xu-bo 3

(¹College of Horticulture and Landscape Architecture,Hainan University, Danzhou, Hainan 571737,China; ² State Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093; ³Boda Orchid Technology Company Ltd., Haikou 571101 )

Received:2007-12-17 Revised:2008-02-22 Online:2008-04-25 Published:2008-04-25
Contact: SONG Xi-qiang

摘要/Abstract

摘要：

为探索菌根技术在兰科植物保育中的应用，以野生五唇兰的内生菌根真菌和五唇兰组培苗为材料，进行组培瓶苗和盆栽苗的菌根化研究。利用固体培养的菌丝体与五唇兰无菌组培苗进行共生培养，15 d后，根染色法检测结果表明，真菌能够成功侵染根，菌根化苗的移栽成活率均达到100 %（对照为63.33 %）。种植90 d后各菌株处理苗的鲜样质量增长率均高于对照，其中F29菌株处理苗的鲜样质量增长率高于对照31.3 %。在大棚栽培条件下，利用液体菌剂对盆栽苗进行菌根化培养。90 d后各菌株处理苗的成活率和鲜样质量增长率均高于对照，其中F29菌株处理比对照高出35 %，鲜样质量增长率高于对照高33.24 %。

关键词: 兰花, 五唇兰, 内生菌根真菌, 育苗

Abstract:

It aims at the application of mycorrhization technique in the orchid conservation. The endogenetic fungi from the root of the wild plant of Doritis pulcherrima Lindl were inoculated with seedlings of D.pulcherrima in flask and in pot. Firstly, the symbiotic cultivation of aseptic seedlings with the mycelium of solid culture was carried out in flask. According to the method of root coloration, the result showed that the fungi can successfully infect the root of D. pulcherrima seeding after 15d, with 100﹪ survival rate, while that of the control without mycorrhizal fungi was 63.33﹪. After 90 d, the fresh weigh of these seedlings treated with fungi were all higher than the control, especially as for the treatment with fungi F29, there were even 31.3﹪ higher than the control. Secondly, Seedlings were also cultivated with the liquid strain in pot. After 90 d, both the survival rate and the fresh biomass of the mycorrhization seedlings were higher than those of the control. For the mycorrhization seedlings with F29, they have 35﹪ survival rate and 33.24﹪the fresh weigh higher than those of the control, respectively. The results suggested that there was remarkable or significant difference between the inoculated plants and the control.

Key words: orchid, Doritis pulcherrima Lindl., endomycorrhizal fungi, seedling cultivation

中图分类号:

S 682.31

柯海丽;宋希强;;罗毅波;朱国鹏;凌绪柏. 五唇兰菌根化育苗技术[J]. 园艺学报, 2008, 35(4): 571-576.

KE Hai-li;SONG Xi-qiang;;LUO Yi-bo;ZHU Guo-peng;and LING Xu-bo . Seedling Cultivation of Doritis pulcherrima Lindl. with Mycorrhizal Fungi[J]. ACTA HORTICULTURAE SINICA, 2008, 35(4): 571-576.

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