银条根茎离体诱导

园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 873-875.

银条根茎离体诱导

郭东伟^{1, 2};马有志²;周一龙³;闵东红¹;陈耀锋^1*

(¹西北农林科技大学农学院细胞工程实验室, 陕西杨凌712100; ² 中国农业科学院作物科学研究所, 北京100081;³ 中国科学院植物研究所, 北京100093)

收稿日期:2005-09-15 修回日期:2005-12-07 出版日期:2006-08-25 发布日期:2006-08-25

Inducement in Vitro of Rhizome of Stachys floridana Schuttl. ex Benth.

Guo Dongwei^{1, 2}; Ma Youzhi²;Zhou Yilong³;Min Donghong¹;Chen Yaofeng^1*

(¹Cell Engineering Lab, Agronomy College, Northwest A & F University,Yangling, Shaanxi 712100, China; ² Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China; ³ Institute of Botany, the Chinese Academy of Sciences, Beijing 100093, China)

Received:2005-09-15 Revised:2005-12-07 Online:2006-08-25 Published:2006-08-25

摘要/Abstract

摘要： 在无诱导剂的培养基上, 低于4%的蔗糖不能刺激银条(Stachys floridana Schuttl. ex Benth. )产生根茎; 而10%蔗糖的诱导效果在MB和MS培养基间无显著差异。葡萄糖(10% ) 比同浓度蔗糖能显著提高试管根茎数, 但不引起总鲜样质量的增加。每个银条植株在含4%蔗糖的MS培养基附加8.0 mg·L^{- 1} 6-BA和10 mg·L ^{- 1} SA中可诱导产生质量0.45 g的根茎3.33个, 显著优于其它组合。根茎经流式细胞仪检测没有发生倍性变化。

关键词: 银条, 根茎, 诱导剂, 离体诱导, 流式细胞仪

Abstract: Sucrose less than 4% in medium could not stimulate formation of rhizome for Stachys floridana Schuttl. ex Benth. on the medium without revulsant. There was no significant revulsive difference between MS medium and MB medium with 10% sucrose. Glucose could induce significantly more rhizome than sucrose at the same concentration level, but could not make the increasing of gross fresh weight of rhizome. Every plant of Stachys floridana Schuttl. ex Benth. would be induced to produce 3.33 pieces and 0.45 g of rhizomes on
MS medium containing 4% sucrose, 8.0 mg·L ^{- 1} 6-BA and 10 mg·L ^{- 1} SA, which was significantly better than other combinations. The change of ploidy level was not observed in the induced rhizomes according to the result of determination by flow cytometry.

Key words: Stachys floridana Schuttl. ex Benth., Rhizome, Revalsant, Inducement in vitro, Flow cytometry

郭东伟;;马有志;周一龙;闵东红;陈耀锋. 银条根茎离体诱导[J]. 园艺学报, 2006, 33(4): 873-875.

Guo Dongwei;;Ma Youzhi;Zhou Yilong;Min Donghong;Chen Yaofeng. Inducement in Vitro of Rhizome of Stachys floridana Schuttl. ex Benth.[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 873-875.

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