辣椒属5个栽培种部分种质亲缘关系的RAPD分析

园艺学报 ›› 2006, Vol. 33 ›› Issue (4): 751-756.

辣椒属5个栽培种部分种质亲缘关系的RAPD分析

陈学军^{1, 2};陈劲枫^1*;耿红¹;娄群峰¹

(¹南京农业大学园艺学院, 作物遗传与种质创新国家重点实验室, 江苏南京210095; ² 江西省农业科学院蔬菜花卉研究所, 江西南昌330200)

收稿日期:2005-10-08 修回日期:2006-02-27 出版日期:2006-08-25 发布日期:2006-08-25

RAPD Analysis of Phenogenic Relationship in Five Cultivated Capsicum Species

Chen Xuejun^{1, 2};Chen J infeng^1*; Geng Hong¹;Lou Qunfeng¹

(¹National Key Laboratory of Crop Genetics and Germplasm Enhancement, Department of Horticulture, Nanjing Agricultural University, Nanjing, Jiangsu 210095, China; ²Vegetable and Flower Institute, Jiangxi Academy of Agricultural Sciences,Nanchang, Jiangxi 330200, China)

Received:2005-10-08 Revised:2006-02-27 Online:2006-08-25 Published:2006-08-25

摘要/Abstract

摘要： 采用31个10 bp随机RAPD引物对辣椒属(Capsicum ) 5个栽培种31份材料进行PCR扩增,
共扩增出276条带, 其中多态性带244条, 占88.41%。C. annuum 多态性位点比例( PPB) 和Shannon多样性指数( I) 分别为32.97%和0.1599, 表明其遗传多态性较低。31份材料两两不同种质间Jaccard相似系数在0.349～0.952之间, 平均为0.729。聚类分析结果显示: C. annuum 与其它4个栽培种的亲缘关系由近至远分别是C. chinense、C. frutescens、C. pubescens和C. baccatum。对C. annuum 24份不同类型材料聚类分析的结果与形态分类不能完全对应, 说明我国现行主要基于果实形态的变种分类体系不能准确反映C. annuum种质的遗传差异。本研究还发现中国云南西双版纳C. frutescens种质与美洲C. frutescens种质具
有较大的扩增片段差异, 为进一步考证我国云南西双版纳地区也是辣椒起源地之一提供了新的证据。

关键词: 辣椒属, 遗传差异, RAPD, 聚类分析

Abstract: RAPD analysis was conducted in 31 accessions from 5 cultivated species in Capsicum. Thirtyone primers generated 276 scorable RAPD bands ofwhich 244 were polymorphic. The percentage of polymorphic loci ( PPB) and Shannonps information index ( I) in C. annuum were 32.97% and 0.1599 respectively, indicating the genetic diversitywithin C. annuum was low. Jaccard's similarity between pairs of accessionsranged from 0.349 to 0.952 with a mean of 0.729. The results from cluster analysis showed that the genetic distances of C. annuum with other four species beginningwith nearerwere C. chinense, C. frutescens, C. pubescens and C. baccatum. The cluster formed by the 24 accessions of C. annuum did not fully correspond to the current morphologic classification, suggesting that the genetic differences among those accessions of C. annuum could not be precisely reflected by the current taxonomic system. Great diversity of amp lification bands was observed between C. baccatum accession from Xishuangbanna, China and that from America, which provided us with new evidence to the argument that if the Xishuangbanna area in China was also a place for pepper origin.

Key words: Capsicum, Genetic diversity, RAPD marker, Cluster analysis

陈学军;;陈劲枫;耿红;娄群峰. 辣椒属5个栽培种部分种质亲缘关系的RAPD分析[J]. 园艺学报, 2006, 33(4): 751-756.

Chen Xuejun;;Chen J infeng;Geng Hong;Lou Qunfeng. RAPD Analysis of Phenogenic Relationship in Five Cultivated Capsicum Species[J]. ACTA HORTICULTURAE SINICA, 2006, 33(4): 751-756.

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