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园艺学报 ›› 2008, Vol. 35 ›› Issue (5): 687-692.

• 蔬菜 • 上一篇    下一篇

番茄LeEIL1基因表达工程菌的构建及表达分析

李 季;李正国*;罗安才;杨迎伍;邓 伟   

  1. (重庆大学生物工程学院基因工程研究中心,重庆市高校功能基因与调控新技术重点实验室,重庆400044)
  • 收稿日期:2007-11-05 修回日期:2008-02-28 出版日期:2008-05-25 发布日期:2008-05-25
  • 通讯作者: 李正国

Construction of the Expression Vectors of Tomato LeEIL1 and Their Expression in Engineering Strains

LI Ji, LI Zheng-Guo*, LUO An-Cai, YANG Ying-Wu, and DENG Wei
  

  1. (Genetic Engineering Research Center, College of Bioengineering, Chongqing University, Key Laboratory of Functional Gene and Regulation Technologies under Chongqing Municipal Education Commission, Chongqing 400044, China)
  • Received:2007-11-05 Revised:2008-02-28 Online:2008-05-25 Published:2008-05-25
  • Contact: LI Zheng-Guo

摘要:

以番茄果实为材料,通过RT-PCR扩增出番茄leEIL1基因,并构建了3种表达载体。表达结果比较分析表明,在以pPIC9k为表达载体,KM71毕赤酵母为宿主细胞的真核表达体系中,LeEIL1蛋白质的表达结果明显优于在以pET30a和pET15b为载体,BL21(DE3)plyss大肠杆菌为宿主细胞的原核表达体系中的表达结果。

关键词: 番茄, 乙烯不敏感因子, 原核表达体系, 真核表达体系

Abstract:

EIN3 plays an important role in the ethylene signaling pathway of plant. In the study, the tomato LeEIL1 gene was cloned by RT-PCR from tomato fruit, and three expression vectors of tomato LeEIL1 were constructed. After comparision between the two host strains, it was found that the expression of LeEIL1 was more effective when LeEIL1 was cloned in pPIC9k vector and expressed in Pichia pastoris than cloned in pET30a/pET15b vectors while expressed in E.coli BL21(DE3).

Key words: tomato, ethylene-insensitive factor, prokaryotic expression system, eukaryotic expression system

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