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园艺学报 ›› 2006, Vol. 33 ›› Issue (2): 405-407.

• 研究简报 • 上一篇    下一篇

莴苣高效瞬时表达体系的建立

李 静1, 2;陈 敏2*;刘现伟2;沈法富1;王 鹏2*   

  1. (1 山东农业大学农学院, 作物生物技术重点实验室, 泰安271018; 2 山东大学生命科学学院, 微生物技术国家重点实验室, 济南250100)
  • 收稿日期:2005-07-27 修回日期:2005-12-09 出版日期:2006-04-25 发布日期:2006-04-25

A Highly Efficient System Establishment of Transient Expression in Lettuce

Li Jing1;Chen Min2*;Liu Xianwei2; Shen Fafu1;Wang Peng2*   

  1. (1 The Key Laboratory of Crop Biology of Shandong, School of Agronomy, Shandong Agricultural University, Tai'an 271018,China; 2 The State Key Laboratory of Microbial Technology, School of Life Science, Shandong University, Ji'nan 250100, China)
  • Received:2005-07-27 Revised:2005-12-09 Online:2006-04-25 Published:2006-04-25

摘要: GUS基因作为报告基因, 利用农杆菌真空渗透法, 采取正交试验设计对莴苣(Lactuca sativa L. sp) 的瞬时表达条件进行研究, 建立了高表达水平的瞬时转化体系(乙酰丁香酮200 μmol /L、菌液密度OD600值0.8、真空处理30 min、共培养6 d) 。应用结果表明: 优势组合的表达水平比对照的表达水平提高16.3倍。

关键词: 莴苣, 农杆菌, 真空渗透, GUS, 瞬时表达

Abstract: Using Agrobacterium vacuum infiltration method the plant bivalent expression vector pBI121 containing GUS as a report gene was transformed into lettuce (Lactuca sativa L. sp). Through orthogonal experiment, a highly efficient system of transient expression was observed in plant intact leaves when they were
treated with 200 μmol/L acetosyringone, 0.8 OD600 bacterium density, vacuum 30 min and co-culture 6 days. Transgenic expression level of the optimal condition is improved 16.3 times than that of the control.

Key words: Lactuca sativa, Agrobacteriumtum efaciens, Vacuum infiltration, GUS, Transient expression