‘白核’龙眼种子败育不同时期差异蛋白分析

园艺学报 ›› 2009, Vol. 36 ›› Issue (12): 1733-1740.

‘白核’龙眼种子败育不同时期差异蛋白分析

刘浩^{1, 2} ; 柳燕贞^{1, 2} ; 王静^{1, 2} ; 郑少泉³; 蒋际谋³ ; 陈伟^{1, 2*}

(¹福建农林大学生命科学学院, 福州350002; ² 福建农林大学蛋白质组学研究中心, 福州350002; ³福建省农业科学院果树研究所, 福州350013)

收稿日期:2009-08-23 修回日期:2009-11-26 出版日期:2009-12-25 发布日期:2009-12-25
通讯作者: 陈伟

Analysis of Differentially Expressed Proteins During the Different Stages of Longan Seed Abortion

LIU Hao^{1, 2} ; L IU Yan-zhen^{1, 2 ;} WANG J ing^{1, 2} ; ZHENG Shao-quan³ , JIANG J i-mou³ ; CHEN Wei^{1, 2*}

(¹College of L ife Sciences, Fujian Agriculture and ForestUniversity, Fuzhou 350002, China; ² Proteom ics Research Center, Fujian Agriculture and Forestry University, Fuzhou 350002, China; ³ Fruit Research Institute, Fujian Academy of Agriculture Sciences,Fuzhou 350013, China)

Received:2009-08-23 Revised:2009-11-26 Online:2009-12-25 Published:2009-12-25

摘要/Abstract

摘要： 以‘白核’龙眼种子为试验材料, 比较两种蛋白质的提取方法, 确定了酚抽法提取龙眼种子蛋白。应用蛋白质组学研究技术, 分析了龙眼种子败育4个不同时期的蛋白质组变化, 共发现21个差异蛋白, 其中9个上调表达, 12个下调表达。通过MALD I/TOF /TOF2MS/MS质谱分析和蛋白质数据库检索, 共鉴定出12个差异蛋白, 分别为胞质苹果酸脱氢酶、丙酮酸脱氢酶、磷酸甘油酸变位酶、RuBisCO大亚基结合蛋白α、β亚基、17 kD HSP、抗坏血酸过氧化物酶、胞质抗坏血酸过氧化物酶、半胱氨酸蛋白酶、Desication-related protein以及两个未知蛋白。这些鉴定出的差异蛋白质与能量和物质代谢、分子伴侣功能、自由基清除和抗氧化作用、细胞凋亡等生理过程密切相关, 可能参与了龙眼种子败育过程。

关键词: 龙眼, 种子, 败育, 差异蛋白, 双向电泳(22DE), 质谱分析

Abstract: By comparing with two protein extraction methods, ensured the optimal method for longan seed proteins extraction. Proteomic approaches were used to analyze the changes of differentially expressed proteins during four different stages of longan seed abortion. Twenty-one differentially expressed proteins were detected on 2-DE gels, 9 were up-regulated and 12 were down-regulated. Among these proteins, 12 proteins were identified by MALDI/TOF /TOF-MS/MS and protein database searching. They were cytosolic malate dehydrogenase, Dihydrolipoamide adetyltransferase, 2, 3-bisphosphoglycerate-independent phosphoglycerate mutase, RuB isCO large subunit2binding p rotein subunit beta chlorop last p recursor, RuBisCO large subunit-binding protein subunit beta chloroplast precursor, 17 kD heat shock p rotein, ascorbate peroxidase, cytosolic ascorbate peroxidase, cysteine protease, dessication-related protein, and two unknown protein. These proteins were involved in energy and metabolism, chaperon, antioxidation, apoptosis, which might relate to seed abortion of longan.

Key words: seed, abortion, differentially expressed protein, two-dimensional electrophoresis (2-DE), mass spectrometry analysis

刘浩;;柳燕贞;;王静;;郑少泉;蒋际谋;陈伟;. ‘白核’龙眼种子败育不同时期差异蛋白分析[J]. 园艺学报, 2009, 36(12): 1733-1740.

LIU Hao;;L IU Yan-zhen;;WANG J ing;;ZHENG Shao-quan;JIANG J i-mou;CHEN Wei;. Analysis of Differentially Expressed Proteins During the Different Stages of Longan Seed Abortion[J]. ACTA HORTICULTURAE SINICA, 2009, 36(12): 1733-1740.

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