短暂高温对百合植株抗氧化酶系统的影响

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 509-512.

短暂高温对百合植株抗氧化酶系统的影响

尹慧;陈秋明;何秀丽;义鸣放^*

（中国农业大学观赏园艺与园林系，北京 100094 ）

收稿日期:2006-10-23 修回日期:2007-01-23 出版日期:2007-04-25 发布日期:2007-04-25
通讯作者: 义鸣放

Effects of High Temperature on the Activities of Antioxidant Enzymes in Lily Plants

Yin Hui,Chen Qiu-ming,He Xiu-li, and Yi Ming-fang^*

(Department of Ornamental Horticulture and Landscape Architecture，China Agricultural University, Beijing 100094, China)

Received:2006-10-23 Revised:2007-01-23 Online:2007-04-25 Published:2007-04-25
Contact: Yi Ming-fang

摘要/Abstract

摘要：

以百合栽培品种‘White Heaven'和‘Tiber’为材料，研究了短暂高温胁迫对抗氧化酶（SOD、POD、CAT）活性、相对电导率、丙二醛（MDA）及可溶性蛋白含量的影响，同时对SOD、POD同工酶电泳进行了分析。结果表明：37℃和42℃短暂高温胁迫对两品种形态、相对电解质渗透率没有明显影响，47℃处理下‘Tiber’相对电解质渗透率及MDA含量明显提高。高温胁迫提高了两品种SOD、POD、CAT活性。高温胁迫下两品种SOD同工酶带无增减，只是活性改变。‘White Heaven'存在5条POD同工酶带，‘Tiber’存在2条，高温胁迫后‘White Heaven’POD同工酶表达量高于‘Tiber’。以上结果说明：百合植株可通过提高抗氧化酶活性来抵御一定的高温胁迫（37℃、42℃），不同品种SOD、POD、CAT活性对高温响应方式不同。

关键词: 百合, 高温胁迫, 抗氧化酶, 同工酶, 丙二醛, 可溶性蛋白

Abstract: The effects of short time high temperature on the antioxidant enzymes, content of MDA, soluble protein and relative electric conductivity were investigated in Lilium cultivars ('White Heaven'and'Tiber')．The results showed that the changes of plant morphology, relative electric conductivity and MDA content in two cultivars were slightly affected at 37℃ and 42℃ whereas the level of electric conductivity and MDA content increased obviously under 47℃ specially to 'Tiber'. Heat shock could promote the activities of SOD，POD and CAT compared to control and analysis of SOD and POD isoenzymes revealed that the number of SOD isoenzyme was unchanged in two cultivars while the expression of SOD was affected by heat shock.．There were five bands in 'White Heaven' and two bands of POD isoenzyme in'Tiber' were identified．The POD activity for 'White Heaven' was obvious higher than 'Tiber' after heat shock．These results demonstrated that the lily plants might enhance the activities of antioxidant system to alleviate the damage caused by heat stress however the patterns of antioxidant enzymes to response to high temperature of the two cultivars were different.

Key words: Lily, High temperature stress, Antioxidant enzyme, Isoenzyme, MDA, protein

中图分类号:

S 68

尹慧;陈秋明;何秀丽;义鸣放. 短暂高温对百合植株抗氧化酶系统的影响[J]. 园艺学报, 2007, 34(2): 509-512.

Yin Hui;Chen Qiu-ming;He Xiu-li;and Yi Ming-fang. Effects of High Temperature on the Activities of Antioxidant Enzymes in Lily Plants[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 509-512.

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