https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 493-496.

• 研究简报 • 上一篇    下一篇

番茄抗病基因Tm-22及其等位基因氨基酸序列的比较

姜国勇1*;王林华1;黄婷婷2   

  1. 1莱阳农学院植物基因工程研究所 山东青岛266109;2青岛农业科学院蔬菜研究所 山东青岛266101)
  • 收稿日期:2006-12-28 修回日期:2007-03-23 出版日期:2007-04-25 发布日期:2007-04-25
  • 通讯作者: 姜国勇

Comparison of Amino Acid Sequences on Resistant Gene Tm-22and its Alleles

Jiang Guo-yong1*,Wang Lin-hua1,Huang Ting-ting2   

  1. 1Institute of Plant Gene Engineering,Laiyang Agricultural College,Qingdao, Shandong,266109, China;2 Vegetables Institute, Qingdao Agricultural Academy 266101, Qingdao, Shandong ,China)
  • Received:2006-12-28 Revised:2007-03-23 Online:2007-04-25 Published:2007-04-25
  • Contact: Jiang Guo-yong

摘要:

从4个番茄栽培品种(Lycopersicon esculentum Miller)中获得了Tm-22等位基因的同源克隆,进行序列分析,结果表明:'沙龙'、'渝抗2号'和'97-4'分别与'GCR-267'(Tm-22)、'GCR-236'(tm-2)、以及'GCR-26'(tm-2)有100%的同源性, 而樱桃番茄(Lycopersicon peruvianum. var.cerasiforme)品种'樱红1号'与'GCR-26'(tm-2)仅有98%的同源性;在C-端的LRR结构域中,抗病基因 Tm-22Tm-2 编码蛋白的氨基酸序列与感病基因tm-2存在624和704两个位点的氨基酸差异,即624位点的脯氨酸(P)对应亮氨酸(L)、704位点的甲硫氨酸(M)对应异亮氨酸(I); 在601-790个氨基酸的LRR结构域中,抗病基因Tm-22Tm-2及其秘鲁种(Lycopersicon peruvianum var. dentatumlptm-2三者均与感病基因tm-2的编码蛋白存在23个氨基酸的差异;(4)樱桃番茄品种'樱红1号'与含有tm-2基因的栽培种之间具有同源性关系。

关键词: 番茄, 抗病基因, Tm-22等位基因, 氨基酸序列

Abstract:

Alleles of Tm-22 from 4 different cultivars were cloned. Sequence analysis of them showed that: 1) 'Salong', 'Yukang-2' and '97-4' had 100% identity to reported sequences from 'GCR267'(Tm-22),'GCR236'(Tm-2)and'GCR26'(tm-2), respectively; Cultivar 'yinghong1' had 98% identity to 'GCR26'(tm-2);2) in LRR domain of the encoded proteins, the resistant alleles Tm-22 and tm-2have two amino acid at sites of 624 and 704 different from that of the susceptible alleles tm-2, lptm-2, in which proline (P) changed to leucine (L) and methionine (M) to isoleucine (I); 3) in LRR domain of the encoded proteins between the sites of 601 and 790, 23 amino acid differences were found among Tm-22, Tm-2, lptm-2 and tm-2; 4) tm-2 of Yinghong1 (Lycopersicon esculentum.var.cerasiforme) might be an orthologous gene evolved from Lycopersicon esculentum cultivars.

Key words: Tomato, Resistant gene, Tm-22alleles, Amino acid sequence

中图分类号: