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园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 485-488.

• 研究简报 • 上一篇    下一篇

顶空固相微萃取气—质联用分析香蕉的香气成分

朱 虹1; 陈玉芬2;李雪萍1;李 军1;韩冬芳1; 陈维信1*   

  1. (1 广东省/广州市果蔬保鲜重点实验室, 华南农业大学园艺学院, 广州510642; 2华南农业大学测试中心, 广州510642)
  • 收稿日期:2006-11-16 修回日期:2007-03-06 出版日期:2007-04-25 发布日期:2007-04-25
  • 通讯作者: 陈维信

Determ ina tion of Volatiles in Harvested Banana Fruit by HS-SPME andGC-MS

ZHU Hong1, CHEN Yu-fen2,LI Xue-ping1, LI Jun1, HAN Dong-fang1,and CHEN Wei-xin1*   

  1. (1Guangdong Key Laboratory for Postharvest Science and Technology, College of Horticulture, South China AgriculturalUniversity, Guangzhou 510642, China; 2 Instrum ental Analysis & Research Center, South China Agricultural University, Guangzhou 510642, China)
  • Received:2006-11-16 Revised:2007-03-06 Online:2007-04-25 Published:2007-04-25
  • Contact: CHEN Wei-xin

摘要: 采用顶空固相微萃取技术提取不同成熟阶段香蕉的香气成分, 再经气质联用进行分析鉴定。
着重研究3种极性不同的萃取头对香蕉香气成分萃取的影响, 优化了分析条件, 建立了稳定的萃取与气质联用分析测定的方法。研究结果表明, 香蕉处于不同成熟阶段香气成分的种类和相对含量具有明显差异,在香蕉绿熟阶段分析测定适宜采用DVB /CAR /PDMS型萃取头, 黄熟阶段采用PDMS型萃取头, 过熟阶段采用CW /DVB型萃取头。

关键词: 香蕉, 香气成分, 顶空—固相微萃取, 气质联用

Abstract:

The headspace solid-phase microextraction coup led with capillary gas chromatography-mass spectrometry (GC-MS) was used for the determination of volatiles in harvested banana fruit (Musa spp.‘Baxi’) during ripening. The fiber coating, as one of the main parameters that influence the extraction effect,was extensively studied and op timized. A steadymethod of HS-SPME coup led with GC - MS for banana volatiles determination was established. The results showed that the constituent and amount of volatiles in banana fruitwere shown to be of great difference during ripening. Therefore, it was very necessary and important to select appropriate fibers and op timize GC-MS condition for the extraction and determination of volatiles in banana at different stages of ripening.

Key words: Banana, Volatile, HS-SPME, GC-MS

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