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园艺学报 ›› 2007, Vol. 34 ›› Issue (2): 366-370.

• 蔬菜 • 上一篇    下一篇

钙处理对乙烯诱导的番茄离体花柄脱落的抑制作用

许 涛;李天来*;齐明芳   

  1. (沈阳农业大学园艺学院, 辽宁省设施园艺重点实验室, 沈阳110161)
  • 收稿日期:2006-11-30 修回日期:2007-01-19 出版日期:2007-04-25 发布日期:2007-04-25
  • 通讯作者: 李天来

Physiological Regulation of Calcium in Tomato Pedicel in Vitro During Abscission Induced by Ethylene

XU Tao, LI Tian-lai*, and QI Ming-fang   

  1. (College of Horticulture, Shenyang Agricultural University, Liaoning Province Key Laboratory of Protected Horticulture, Shenyang 110161, China)
  • Received:2006-11-30 Revised:2007-01-19 Online:2007-04-25 Published:2007-04-25
  • Contact: LI Tian-lai

摘要: 以番茄‘辽园多丽’的小花为材料, 研究外源乙烯( 20μL ·L-1 ) 处理下, 花柄脱落过程
中, 离区微粒体膜Ca2+ -ATPase、羧甲基纤维素钠酶(CMC-Na) 、内切多聚半乳糖醛酸酶( Endo-PG) 和果胶脂酶( PE) 等活性的变化。结果表明: 外源乙烯加速了花柄的脱落, 刺激了其离区的Ca2+ -ATPase活性先升高后下降, 随后离区CMC-Na、Endo-PG、PE活性升高; 钙离子专一性螯合剂EGTA和钙调素拮抗剂TFP均在一定程度上抑制了乙烯诱导的上述反应, 表明细胞内Ca2+与CaM参与了乙烯反应。

关键词: 番茄, 脱落, 乙烯, 水解酶,

Abstract: In an experiment, pedicels of Lycopersicon esculenturm Mill. ‘Liaoyuanduoli’were used to study the changes of Ca2+ -ATPase activity, abscission rate and the activity of Endo-PG, PE, CMC-Na enzymes in abscission zone after the pedicelswere incubated in exogenous ethylene (20μL·L-1 ). The results
showed that ethylene could accelerate the abscission during incubation, Ca2+ -ATPase activity first rose sharply and then declined quickly. Subsequently, there was a increase in the activity of Endo-PG, PE, CMC-Na enzyme in abscission zone. Calmodulin antagonist trifluoperizine (TFP) and Ca2+ chelator EGTA both inhibited the effects induced by ethylene to a certain extent. It is concluded that Ca2+ and CaM participate in the reaction of exogenous ethylene.

Key words: Tomato, Abscission, Ethylene, Hydrolysis enzyme, Calcium

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