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园艺学报 ›› 2020, Vol. 47 ›› Issue (3): 562-570.doi: 10.16420/j.issn.0513-353x.2019-0411

• 研究报告 • 上一篇    下一篇

利用远缘杂交创制抗虫长豇豆新种质

邵景杰,潘 磊,万何平,郭 瑞,陈 高,谢 倩,任三国,卢 爽,严 牧,陈禅友*   

  1. 江汉大学生命科学学院,湖北省豆类(蔬菜)植物工程技术研究中心,武汉 430056
  • 出版日期:2020-03-25 发布日期:2020-03-25
  • 基金资助:
    湖北省技术创新专项重大项目(2017ABA147);湖北省自然科学基金项目(2017CFB442);湖北省食用豆类自然科技资源中心主任基金项目(2017-ZR01)

Germplasm Innovation of Pest-resistant Asparagus Bean by Interspecific Crosses

SHAO Jingjie,PAN Lei,WAN Heping,GUO Rui,CHEN Gao,XIE Qian,REN Sanguo,LU Shuang,YAN Mu,and CHEN Chanyou*   

  1. School of Life Sciences,Jianghan University,Hubei Province Engineering Research Center for Legume Plants,Wuhan 430056,China
  • Online:2020-03-25 Published:2020-03-25

摘要: 开展了长豇豆(Vigna unguiculata L. ssp. sesquipedalis)与其近缘种饭豆[V. umbellata(Thunb.)Ohwi & Ohashi]的远缘杂交,对亲本和杂交高世代(F7)进行了田间抗虫水平和荚果性状观测鉴定。结果表明:(1)饭豆品种的田间抗虫水平普遍高于长豇豆品种;(2)在长豇豆和饭豆远缘杂交的全分离谱世代(F6)363 个株系中,初选了 41 个虫害发生较轻的单株,组成 F7 代供后续鉴定;(3)田间试验观测到 F7 代株系抗虫水平有较大分离,复选出 7 个高抗水平的重组株系;结合单荚质量、荚长和籽粒密度等荚果相关性状数据特征,决选出在营养生长期对斜纹夜蛾、甜菜夜蛾等主要害虫综合抗性达到高抗(HR)水平且荚果性状近似于长豇豆的株系 2 个,实现了长豇豆抗虫的种质创新。

关键词: 长豇豆, 虫害指数, 种间杂交, 种质创新

Abstract: The interspecific crosses of asparagus bean(Vigna unguiculata L. ssp. sesquipedalis)and its related species of rice bean[V. umbellata(Thunb.)Ohwi & Ohashi]were carried out. The pest-resistance level and pod traits of the parents and their 7th generations were identified and evaluated. The results showed that the pest-resistance levels of rice bean varieties were generally higher than that of asparagus bean varieties in the field. Meanwhile,41 individual plants showing resistance to pest were selected from 363 lines containing all kinds of segregation in F6 generation of interspecific crosses between asparagus bean and rice bean. They constituted F7 generation. The field experiment results revealed that the pest-resistance levels of F7 offspring were separated significantly and seven recombinant lines with high resistance(HR)level were re-selected. Finally,according to the characteristics of pod-related traits such as pod length and grain density,two within the seven lines with HR level to Prodenia litura Fabricius and Spodoptera exigua Hübner at vegetative growth stage,and similar pod traits to asparagus bean were selected. Therefore,the germplasm innovation of asparagus bean pest-resistance was realized.

Key words: asparagus bean, pest index, interspecific cross, germplasm innovation

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