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园艺学报 ›› 2006, Vol. 33 ›› Issue (1): 125-127.

• 研究简报 • 上一篇    下一篇

PGIP基因的克隆及全序列分析

李广平1;房经贵1;蔡斌华1;章 镇1;张长青2   

  1. (1 南京农业大学园艺学院, 南京210095; 2 金陵科技学院园艺系, 南京210038)
  • 收稿日期:2004-12-08 修回日期:2005-05-12 出版日期:2006-02-25 发布日期:2006-02-25

Cloning and Sequencing of PGIP Gene from Prunus mume Sieb

Li Guangping1; Fang Jinggui1; Cai Binhua1; Zhang Zhen1;Zhang Changqing2   

  1. (1College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China; 2Department of Horticulture, Jinling Institute of Technology, Nanjing 210038, China)
  • Received:2004-12-08 Revised:2005-05-12 Online:2006-02-25 Published:2006-02-25

摘要: 通过PCR扩增, 从梅基因组中得到1条全长1 192 bp的多聚半乳糖醛酸酶抑制蛋白( PGIP)基因序列。该序列包含有1个完整的开放阅读框和1个内元。比对结果表明, 克隆到的序列与桃、马哈利樱桃中相应序列一致度分别为96%和95% , 其蛋白质序列与桃、马哈利樱桃的蛋白质序列一致度分别为97%和94%。该蛋白质序列中包含着一段亮氨酸重复序列。

关键词: 梅, PGIP基因, 克隆, 序列分析

Abstract: A 1 192 bp sequence of polygalacturonase-inhibiting p rotein ( PGIP) gene(Genbank locus:AY764131) was isolated by polymerase chain reaction ( PCR) from Prunus m um e. This sequence had a intron and a full open reading frame encoding the polygalacturonase-inhibiting protein. Its gene and derived protein were both highly homologous to those from Prunus persica and Prunus mahaleb. A conserved leucinerich fragment had existed in the derived protein sequence.

Key words: Prunus mume, PGIP gene, Cloning, Sequencing