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园艺学报 ›› 2019, Vol. 46 ›› Issue (12): 2429-2436.doi: 10.16420/j.issn.0513-353x.2019-0407

• 研究报告 • 上一篇    下一篇

马铃薯短花药基因sa1的定位与候选基因预测

王 培1,唐 蝶2,李玉梅3,杨中敏4,祝光涛1,黄三文2,张春芝2,*   

  1. 1云南师范大学马铃薯科学研究院,昆明 650500;2中国农业科学院深圳农业基因组研究所,广东深圳 518120; 3昆明理工大学信息工程与自动化学院,昆明 650500;4西北农林科技大学园艺学院,陕西杨凌 712100
  • 出版日期:2019-12-25 发布日期:2019-12-25
  • 基金资助:
    云南省科技厅高端人才引进计划项目(2013HA025);中国农业科学院科技创新工程项目

Genetic Mapping and Candidate Prediction of the sa1 Gene Controlling Anther Development in Diploid Potatoes

WANG Pei1,TANG Die2,LI Yumei3,YANG Zhongmin4,ZHU Guangtao1,HUANG Sanwen2,and ZHANG Chunzhi2,*   

  1. 1The CAAS-YNNU Joint Academy of Potato Sciences,Yunnan Normal University,Kunming 650500,China;2Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,Guangdong 518120,China;3Faculty of Information Engineering and Automation Kunming University of Science and Technology,Kunming 650500,China;4College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Online:2019-12-25 Published:2019-12-25

摘要: 在前期研究中,从二倍体马铃薯中鉴定了1个短花药突变体sa1,是培育不育系的良好材料。在本研究中,构建了sa1的BC1F2分离群体,通过混池测序的方法发现该性状由1个单基因控制,位于6号染色体0 ~ 33.9 Mb的区间。为了精细定位sa1,进一步扩大遗传群体,通过对重组单株进行基因型和表型分析,最终将sa1定位到1.4 Mb的区间。根据马铃薯参考基因组的注释信息,该区域包含40个基因,并通过转录组测序对候选基因进行了预测。

关键词: 马铃薯, 花药发育, BSA-seq

Abstract: In a previous study,we identified a short anther mutant sa1,which is a potential material for developing male sterile lines. In this study,we constructed a BC1F2 segregating population of sa1. By bulked segregant analysis(BSA-seq),we found this trait was controlled by a single gene which located in a 0–33.9 Mb interval on chromosome No. 6. To further map the sa1 gene,we used more BC1F2 progeny to screen the recombinants. Based on the genotype and phenotype of recombinants,the sa1 gene was narrowed to a 1.4 Mb interval. According to the annotation information of the potato reference genome,this region contains 40 genes. In addition,we predicted the candidate genes using the transcriptome data from normal and short anthers.

Key words: potato, anther development, BSA-seq

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