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园艺学报 ›› 2019, Vol. 46 ›› Issue (12): 2397-2405.doi: 10.16420/j.issn.0513-353x.2018-1033

• 研究报告 • 上一篇    下一篇

东北冷寒产区苹果褪绿叶斑病毒检测及其分子多样性分析

陈雅寒1,2,孙平平1,马 强1,*,赵明敏1,武占敏3,李正男1,*   

  1. 1内蒙古农业大学园艺与植物保护学院,呼和浩特 010018;2西北农林科技大学植物保护学院,陕西杨凌 712100;3鄂尔多斯市植保检疫站,内蒙古鄂尔多斯 017000
  • 出版日期:2019-12-25 发布日期:2019-12-25
  • 基金资助:
    国家自然科学基金地区科学基金项目(31860540);内蒙古农业大学高层次人才科研启动金项目(NDYB2018-3)

Detection and Diversity Analysis of Apple chlorotic leaf spot virus in Northeast China

CHEN Yahan1,2,SUN Pingping1,MA Qiang1,*,ZHAO Mingmin1,WU Zhanmin3,and LI Zhengnan1,*   

  1. 1College of Horticulture and Plant Protection,Inner Mongolia Agricultural University,Huhhot 010018,China;2College of Plant Protection and Shaanxi Key Laboratory of Molecular Biology for Agriculture,Northwest A & F University,Yangling,Shaanxi 712100,China;3Plant Protective Station of Ordos,Ordos,Inner Mongolia 017000,China
  • Online:2019-12-25 Published:2019-12-25

摘要: 采用RT-PCR对从东北地区和内蒙古自治区8个采样点采集的165份小苹果枝条样品进行了苹果褪绿叶斑病毒(Apple chlorotic leaf spot virus,ACLSV)的检测,ACLSV的平均检出率为60.6%,说明ACLSV在中国东北和内蒙古自治区普遍发生。对其中25份样品中ACLSV的外壳蛋白(coat protein,CP)基因进行了克隆和测序,结果表明25个ACLSV分离物CP基因核苷酸序列一致性为84.0% ~ 99.7%,编码蛋白氨基酸序列一致性为90.7% ~ 99.5%;与其他ACLSV分离物CP基因核苷酸一致性为67.7% ~ 99.7%,氨基酸一致性为71.0% ~ 99.5%。世界范围内已报道的ACLSV分离物分成B6、P205、SHZ 和Ta Tao 5等4个组,本研究中的25个ACLSV分离物分别属于B6、P205和SHZ组;在25个ACLSV分离物间无重组事件。

关键词: 苹果, 苹果褪绿叶斑病毒, CP, 一致性, 系统发育进化分析, 重组

Abstract: In order to clarify the incidence and diversity of Apple chlorotic leaf spot virus(ACLSV)in northeast China and Inner Mongolia,a total of 165 apple leaf samples were collected from 8 sampling points in these areas and tested by reverse transcription-polymerase chain reaction. The average incidence of ACLSV among the collected samples was 60.6%,suggesting its wide spread in the surveyed regions. The fragments of the complete coat protein(CP)genes generated from 25 samples were cloned and sequenced. Pairwise comparison analysis showed that the nucleotide and amino acid sequence identities of CP genes of the 25 isolates ranged from 84.0%–99.7% and 90.7%–99.5%,respectively,while they shared the nucleotide and amino acid sequence identities with another 13 ACLSV isolates of 67.7%–99.7% and 71.0%–99.5%,respectively. The ACLSV isolates worldwide were grouped into four groups(B6,P205,SHZ and Ta Tao 5),and the 25 ACLSV isolates were located in the groups B6,P205 and SHZ. No significant evidence of recombination was found among the 25 complete CP sequences.

Key words: apple, ACLSV, CP, identity, phylogenetic analysis, recombination

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