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园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 1059-1062.

• 新技术与方法 • 上一篇    下一篇

马铃薯总RNA提取和鉴定方法的改进

白云凤; 郭志华; 白冬梅;王小琦; 张维锋*
  

  1. (山西省农业科学院作物遗传研究所, 太原030031)
  • 收稿日期:2007-01-15 修回日期:2007-04-28 出版日期:2007-08-25 发布日期:2007-08-25

An Improved Method for Extraction and Identification of Potato RNAs

BAI Yun-feng; GUO Zhi-hua; BAI Dong-mei; WANG Xiao-qi; ZHANGWei-feng*   

  1. (Crop Genetics Institute, Shanx i Academ y of Agricultural Sciences, Taiyuan 030031,China)
  • Received:2007-01-15 Revised:2007-04-28 Online:2007-08-25 Published:2007-08-25

摘要: 对植物总RNA的提取和鉴定方法作了改进。以马铃薯叶片和茎为材料,分别用常规苯酚法和改进法提取总RNA,通过甲醛变性胶和非变性胶及紫外光谱分析,表明用改进法提取的总RNA与常规苯酚法无明显差异,RNA完整性良好,可以用普通琼脂糖凝胶代替甲醛变性胶电泳分析RNA的完整性。RT-PCR结果表明,以改进法提取的总RNA可以用于分子克隆和基因表达分析等分子生物学实验。利用改进法提取植物总RNA,能降低成本,节约时间,也避免了使用DEPC和甲醛等试剂。

关键词: 马铃薯, 总RNA提取

Abstract: An improved method of extracting and identifying total RNAs from potato leaf and stem was put forward. The electrophoretic patterns and absorbance at 260 nm and 280 nm in a UV-Vis spectrophotometer revealed that the total RNAs of potato extracted by this method has good completeness and high purity. Furthermore, the result of RT-PCR indicated that the RNAs prepared with this improved method could meet the needs of most molecular biological experiment including gene cloning and expression analysis.

Key words: Potato, Total RNAs extraction