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园艺学报 ›› 2019, Vol. 46 ›› Issue (3): 577-589.doi: 10.16420/j.issn.0513-353x.2018-0430

• 研究报告 • 上一篇    下一篇

香蕉与B基因组相关的SCAR标记研究

王 芳1,牛玉清2,黄秉智3,崔广娟1,张珂恒1,曾莉莎1,刘文清1,吕 顺1,*,郭权香1,何建齐1   

  1. (1东莞市香蕉蔬菜研究所,广东东莞 523061;2华南农业大学园艺学院,广州 510642;3广东省农业科学院果树研究所,广州 510640)
  • 出版日期:2019-03-25 发布日期:2019-03-25

WANG Fang1,NIU Yuqing2,HUANG Bingzhi3,CUI Guangjuan1,ZHANG Keheng1,ZENG Lisha1,LIU Wenqing1,Lü Shun1,*,GUO Quanxiang1,and HE Jianqi1

WANG Fang1,NIU Yuqing2,HUANG Bingzhi3,CUI Guangjuan1,ZHANG Keheng1,ZENG Lisha1,LIU Wenqing1,Lü Shun1,*,GUO Quanxiang1,and HE Jianqi1   

  1. (1Dongguan Banana and Vegetable Institute,Dongguan,Guangdong 523061,China;2College of Horticulture,South China Agricultural University,Guangzhou 510642,China;3Institute of Fruit Tree Research,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China)
  • Online:2019-03-25 Published:2019-03-25

摘要:

香蕉(Musa L.)是由2个二倍体野生种Musa acuminata Colla(AA基因型)和Musa balbisiana Colla(BB基因型)种内或种间杂交进化而来,其B基因组中带有重要的优良基因。利用与香蕉B基因组相关的gypsy-IRAP分子标记,成功开发了一对SCAR引物,适用于鉴定尖叶蕉(AAw)、长梗蕉(BB)、香牙蕉(AAA)、贡蕉(AAcv)、大蕉、粉蕉(ABB)、粉大蕉(ABB)、龙牙蕉(AAB)以及四倍体香蕉(AAAB)等是否含有B基因组。

关键词: 香蕉, B基因组, SCAR分子标记, IRAP标记

Abstract:

The banana cultivars are originated from intra- and interspecific hybridization of two wild diploid species:Musa acuminate Colla and Musa balbisiana Colla,contributing the A and B genomes,respectively. The B genome contains important and good genes. A sequence characterized amplified region(SCAR)marker was successfully developed from the inter-retrotransposon amplified polymorphism (IRAP)marker named gypsy-IRAP related to banana B genome,and it was found to be able to identificate B genome of banana cultivars with different genotypes such as AAw,BB,AAcv,ABB,AAB,AAAB.

Key words: Musa, B genome, SCAR marker, IRAP marker

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