关键词: 茄子斑驳紫花病, siRNA高通量测序, 分子鉴定, 烟草轻型绿花叶病毒, 番茄斑驳花叶病毒

Abstract: To identify the virus causing eggplant purple mottle flower disease，high-throughput sequencing of small interfering RNA（siRNA）was performed with eggplant samples，and then the RNA virus was screened with bioinformatics methods. Results showed that genomic information of Tobacco mild green mosaic virus（TMGMV）and Tomato mottle mosaic virus（ToMMV）were detected in eggplant samples. To confirm the results，total RNAs were analyzed by reverse transcription（RT）-PCR to amplify the full-length coat protein（CP）gene of 500 bp TMGMV and 482 bp ToMMV. Blast analysis confirmed the identity of the virus，showing 98%–100% and 100% similarity to TMGMV-CP and ToMMV-CP sequence，respectively. Twenty-three suspected mottled eggplant samples from Shandong，Henan，Liaoning，and Hebei provinces were tested with specific primers 524-F/R and ToMMV-F/R. The expected 500 bp TMGMV-CP fragment was amplified from all 23 samples，and 289 bp ToMMV-CP fragment was amplified from 20 samples. In pathogenicity test，all leaves of inoculated eggplant，tomato and pepper，and flowers of eggplant showed the same symptoms as the field. The results showed that TMGMV and ToMMV were identified in the eggplant mottled purple flower disease.

Key words: eggplant purple mottle virus disease, siRNA high-throughput sequencing, molecular identification, Tobacco mild green mosaic virus, Tomato mottle mosaic virus