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园艺学报 ›› 2019, Vol. 46 ›› Issue (2): 356-364.doi: 10.16420/j.issn.0513-353x.2018-0605

• 研究报告 • 上一篇    下一篇

双孢蘑菇担子与担孢子形态发育显微观测

赵建霞1,2,冯伟林2,金群力2,沈颖越2,宋婷婷2,蔡为明2,*   

  1. 1浙江师范大学化学与生命科学学院,浙江金华 321004;2浙江省农业科学院园艺研究所,杭州 310021
  • 出版日期:2019-02-25 发布日期:2019-02-25
  • 基金资助:
    浙江省农业新品种选育重大科技专项(2016C02057)

Microscopic Observation of the Morphological Development of Basidia and Basidiospores in Agaricus bisporus

ZHAO Jianxia1,2,FENG Weilin2,JIN Qunli2,SHEN Yingyue2,SONG Tingting2,and CAI Weiming2,*   

  1. 1College of Chemistry and Life Science,Zhejiang Normal University,Jinhua,Zhejiang 321004,China;2Institute of Horticulture,Zhejiang Academy of Agricultural Sciences,Hangzhou 310021,China
  • Online:2019-02-25 Published:2019-02-25

摘要: 利用光学显微镜、共聚焦显微镜和扫描电子显微镜观察双孢蘑菇(Agaricus bisporus)担子(basidia)、担孢子(basidiospores)在不同发育阶段的微观形态特征。担子呈近圆柱体紧密簇状生长,不同发育时期菌褶中不同类型担子的比例并非持续不变,且发育早期(孢子梗形成前)的担子顶部有膜状物(即担子顶膜,Apical Membrance of Basidia,AMB)。担子发育过程中两个细胞核在担子中部融合,融合的单细胞核向担子顶部迁移并发生减数分裂,最终在担子顶部形成4个单倍体核,第二次分裂有时存在不同步性,使担子头部出现3核现象,减数分裂后的4个单倍体核最终平均地移入担孢子中。担孢子壁为两层,担孢子内呈现大小不均的脂滴状物质。

关键词: 双孢蘑菇, 形态观察, 核相变化

Abstract: Optical microscopy,confocal microscopy and scanning electron microscopy were used to observe the micromorphological characteristics of basidia and basidiospores at different stages of development in Agaricus bisporus. The basidia grows closely together and the proportion of different types of basidia in the gill at different developmental stages is not sustained. In the early stage of development,wich is the top of the basidium was covered with membrane(Apical Membrance of Basidium,AMB). In basidium development,the two nuclei fused in the middle of the basidium,and the nuclear phase of the basidium changed during its development. The formation of mononuclear cells migrated back to the top of the basidium and meiosis took place. Finally,four nuclei were formed at the top of the basidium. Sometimes,the second meiosis was different and the phenomenon of three nuclei appeared in the head of the basidia due to the non-synchrony of the secondary mitosis. After meiosis,an average of four nuclei migrated to the sporozoites. Basidiospores wall have two layers. The basidiospores contain lipid droplets of uneven sizes.

Key words: Agaricus bisporus, morphological observation, nuclear phase change

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