https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2018, Vol. 45 ›› Issue (5): 977-987.doi: 10.16420/j.issn.0513-353x.2017-0441

• 研究报告 • 上一篇    下一篇

菊花抗白色锈病相关基因CmDREBa-2的克隆及表达分析

熊超明1,赵兴华2,贾红梅1,延 昕1,毛洪玉1,*   

  1. (1沈阳农业大学林学院,沈阳 110866;2辽宁省农业科学院花卉研究所,沈阳 110161)
  • 出版日期:2018-05-25 发布日期:2018-05-25

Cloning and Expression Analysis of CmDREBa-2,A Resistance-related Gene to Chrysanthemum White Rust

XIONG Chaoming1,ZHAO Xinghua2,JIA Hongmei1,YAN Xin1,and MAO Hongyu1,*   

  1. (1College of Forestry,Shenyang Agricultural University,Shenyang 110866,China;2Institute of Flowers,Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)
  • Online:2018-05-25 Published:2018-05-25

摘要: 以菊花(Chrysanthemum morifolium)免疫白色锈病品种‘C029’为试验材料,基于白色锈病病原菌诱导的菊花转录组数据库,采用RACE(rapid-amplification of cDNA ends)方法克隆得到菊花DREB(Dehydration responsive element binding protein)基因,命名为CmDREBa-2。对其进行生物信息学分析和表达分析,结果表明:CmDREBa-2开放阅读框(ORF)全长291 bp,编码96个氨基酸;预测CmDREBa-2蛋白相对分子质量为11 159.04,pI为11.34,是一个定位于细胞核、亲水的不稳定蛋白;氨基酸序列和结构分析显示该蛋白含有1个由49个氨基酸残基组成的AP2保守结构域,所以该蛋白属于AP2/EREBP家族;进化树分析表明CmDREBa-2属于DREB家族的A-1组,CmDREBa-2蛋白与菊花的其他两个DREB类蛋白CmDREBa和CmDREBb的同源性较高;菊花‘C029’接种白色锈病病原菌6 h后,CmDREBa-2表达量达到最高,约为未接菌对照的34倍;用水杨酸(SA)、茉莉酸甲酯(MeJA)和乙烯利(ETH)处理‘C029’菊花叶片,结果表明CmDREBa-2受3种激素的诱导表达。

关键词: 菊花, CmDREBa-2, 基因克隆, 生物信息学分析, 表达分析

Abstract: Based on the information of transcriptome database derived from‘C029’,a Puccinia horiana Henn. immune Chrysanthemum morifolium cultivar,the cDNA sequence of DREB(Dehydration responsive element binding protein)was cloned. This DREB gene was named CmDREBa-2. Sequence analysis showed that the open reading frame(ORF)of CmDREBa-2 was 291 bp,encoding 96 amino acids. The molecular weight of the predicted protein was 11 159.04 and the pI value was 11.34. The protein was located in the nucleus,and it was a hydrophilic and unstable protein. The transcription factor of CmDREBa-2 contained the AP2 DNA binding domain which was composed by 49 amino acids. The results of phylogenetic analysis showed that the CmDREBa-2 belonged to the DREB family of A-1 and it had a close relationship with CmDREBa and CmDREBb from C. morifolium. The expression level of CmDREBa-2 reached a maximum at 6 h after Puccinia horiana Henn. infection,with 34 times as much as the control. We also found that the expression of CmDREBa-2 was induced by SA,MeJA and ETH.

Key words: chrysanthemum, CmDREBa-2, cloning, bioinformatics analysis, expression analysis

中图分类号: