https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 995-998.

• 研究简报 • 上一篇    下一篇

不同叶龄苹果叶片抗坏血酸含量与其代谢相关酶活性的比较

马春花1; 马锋旺1, 2*;李明军1;韩明玉1;束怀瑞1, 2
  

  1. (1 西北农林科技大学园艺学院, 陕西杨凌712100; 2 山东农业大学园艺科学与工程学院, 山东泰安271018)
  • 收稿日期:2006-11-09 修回日期:2007-05-25 出版日期:2007-08-25 发布日期:2007-08-25

Compar isons of Ascorbic Ac id Contents and Activ ities of Metabolism Relative Enzymes in Apple Leaves of Various Ages

MA Chun-hua1 ;MA Feng-wang1, 2*;Li Ming-jun1 ;HAN Ming-yu1 ;SHU Huai-rui1, 2
  

  1. (1College of Horticulture, N orthwest A & F University, Yangling, Shaanxi 712100, China; 2College of Horticulture Science andEngineering, Shandong Agricultural University, Taipan, Shandong 271018, China)
  • Received:2006-11-09 Revised:2007-05-25 Online:2007-08-25 Published:2007-08-25

摘要: 以‘嘎拉’苹果(Malus domestica Borkh. ‘Gala’) 叶片为试材, 研究了幼叶、展开叶、成熟
叶、衰老叶4个不同时期叶片的抗坏血酸(AsA) 含量、合成和代谢相关酶活性及其相互之间的关系。结
果表明, 不同叶龄苹果叶片总抗坏血酸和AsA含量的变化趋势一致, 均在成熟期最高, 衰老期开始下降,
AsA关键合成酶GalLDH 活性表现出相应的变化。同时, 有助于AsA 再生的脱氢抗坏血酸还原酶
(DHAR) 、单脱氢抗坏血酸还原酶(MDHAR) 、谷胱甘肽还原酶(GR) 活性均在叶片衰老期最低, 并伴有
抗坏血酸过氧化物酶(APX) 活性的下降和H2O2的大量积累, 使抗坏血酸氧化程度在衰老期也达到最大。

关键词: 苹果, 叶片, 叶龄, 抗坏血酸

Abstract: Ascorbic acid (AsA) metabolism, including AsA content, metabolism relative enzymes ac
tivities and relationship s among them, was investigated in leaves of app le (Malus domestica Borkh. ‘Gala’)
of various ages, i.e. young leaves, expanding leaves, mature leaves, aged leaves. The results showed that to
talAsA content and AsA content changed accordingly, reaching maximum in mature leaves and minimum in
aged leaves, and changes of GalLDH activitywere accordantwith AsA content. W ith the decline ofAPX ac
tivities and accumulation of H2O2 , the activities ofMDHAR, DHAR and GR which conduce to regeneration
of AsA were lowest at aged leaves, so as to the extent of AsA oxidation reached maximum in aged leaves.

Key words: Apple, Leaf, Leaf age, Ascorbic acid