GhMADS3基因组成型表达对矮牵牛花形和花色的影响

园艺学报 ›› 2007, Vol. 34 ›› Issue (4): 985-990.

GhMADS3基因组成型表达对矮牵牛花形和花色的影响

郑亚东¹^*;郭余龙^1*;陈　旭²;李艳冬¹；欧建龙³；李名扬^1**

(¹西南大学园艺园林学院, 重庆400715; ²重庆市农作物生物工程中心, 重庆400055; ³重庆市农业技术推广总站,重庆400020)

收稿日期:2007-02-13 修回日期:2007-05-28 出版日期:2007-08-25 发布日期:2007-08-25

Effects of GhMADS3 Gene Constitutive Expression on Flower Shape and Color of Petunia

ZHENG Ya-dong¹^*； GUO Yu-long¹^*； CHEN Xu²； LI Yan-dong¹； OU Jian-long³； and LI Ming-yang^1**

(¹College of Horticulture and Landscape, Southwest University, Chongqing 400715, China; ²Chongqing Center of Bio-engineering for Crops, Chongqing 400055, China; ³Chongqing Academy of Agricultural Technology Promotion, Chongqing 400020, China)

Received:2007-02-13 Revised:2007-05-28 Online:2007-08-25 Published:2007-08-25

摘要/Abstract

摘要：

利用农杆菌介导法将GhMADS3基因转入矮牵牛。对14株转基因植株进行分析鉴定, GUS染

色表明外源基因在转基因植株中表达, PCR检测显示GhMADS3整合到矮牵牛的基因组中, RT-PCR分析表

明GhMADS3在转基因植株中表达。转基因植株花器官普遍较野生型小, 花萼膨大、顶尖向内弯翘, 花冠深

裂且边缘伴有不规则褶皱, 柱头裸露在外, 花器官颜色较野生型变淡甚至完全白化。测定花瓣、花萼中花

色素苷和花萼中叶绿素含量, 结果表明转基因植株均较对照偏低。说明GhMADS3的导入使得矮牵牛花形、

花色发生改变。

关键词: 矮牵牛, 花色, 花形, MADS-box, GhMADS3

Abstract:

An AGAMOUS homologue (GhMADS3) was introduced into petunia via Agrobacterium tume

faciensmediated method. Forteen independent kanamycin-resistant petunia plants were alive. GUS staining revealed that ectopic gene was expressed in transgenic plants. PCR detection showed that the GhMADS3 was integrated into their genomes. The expression of the GhMADS3 was detected by RT-PCR. Constitutive expression

of GhMADS3 resulted in visible phenotypic alterations in floral organ. In transgenic petunia plants, GhMADS3

resulted in the sepals swelling, corolla smaller and corolla deep spliting. It was noteworthy that the color of

transgenic floral organ became weaker or entirely pale. The measuration of anthocyanin and chlorophyll showed

that the content in transgenic plants were greatly lower than wild-type. It also indicated that GhMADS3 in

plants has some relationship with the formation of flower color.

Key words: Petunia, Flower color, Flower shape, MADS-box, GhMADS3

郑亚东;郭余龙;陈　旭;李艳冬;欧建龙;李名扬.

GhMADS3基因组成型表达对矮牵牛花形和花色的影响

[J]. 园艺学报, 2007, 34(4): 985-990.

ZHENG Ya-dong;GUO Yu-long;CHEN Xu;LI Yan-dong;OU Jian-long;and LI Ming-yang.

Effects of GhMADS3 Gene Constitutive Expression on Flower Shape and Color of Petunia

[J]. ACTA HORTICULTURAE SINICA, 2007, 34(4): 985-990.

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